Western blotting using chemiluminescent substrates

Methods in Enzymology

Volumn 541, Issue , 2014, Pages 251-259

  Alegria Schaffer, Alice ^a  

^a Thermo Fisher Scientific   (United States)

Author keywords

Horseradish peroxidase (HRP); Nitrocellulose membrane; Polyvinylidene fluoride (PVDF) membrane; Protein transfer; SDS PAGE; Western blotting

Indexed keywords

POLYVINYLIDENE FLUORIDE; PYROXYLIN; ARTIFICIAL MEMBRANE; HORSERADISH PEROXIDASE; LUMINOL; POLYVINYL DERIVATIVE; PROTEIN;

ARTICLE; ARTIFICIAL MEMBRANE; BINDING SITE; CHEMOLUMINESCENCE; CONCENTRATION (PARAMETERS); ENZYME SUBSTRATE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN TRANSPORT; WESTERN BLOTTING; CHEMISTRY; DEVICES; LUMINESCENCE; METABOLISM; PROCEDURES;

BLOTTING, WESTERN; COLLODION; HORSERADISH PEROXIDASE; LUMINESCENT MEASUREMENTS; LUMINOL; MEMBRANES, ARTIFICIAL; POLYVINYLS; PROTEINS;

EID: 84896960307     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420119-4.00019-7     Document Type: Chapter

References (3)

1. H. Towbin, T. Staehelin, and J. Gordon Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications Proceedings of the National Academy of Sciences of the United States of America 76 1979 4350 4354
2. A. Alegria-Schaffer, A. Lodge, and K. Vattem Performing and optimizing Western blots with an emphasis on chemiluminescent detection Methods in Enzymology 463 2009 573 599
3. One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE).