Effects of continuous passaging on mineralization of MC3T3-E1 cells with improved osteogenic culture protocol

Tissue Engineering - Part C: Methods

Volumn 20, Issue 3, 2014, Pages 198-204

  Yan, Xiang Zhen ^a,b   Yang, Wanxun ^a,c   Yang, Fang ^a   Kersten Niessen, Monique ^a   Jansen, John A ^a   Both, Sanne K ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b SHANDONG UNIVERSITY   (China)

^c Wuhan University   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CELL CULTURE; CELLS; DEPOSITION; GENE EXPRESSION; PHOSPHATASES;

ALKALINE PHOSPHATASE ACTIVITY; AMERICAN TYPE CULTURE COLLECTIONS; BONE SIALOPROTEINS; CALCIUM DEPOSITION; OSTEOGENIC CAPACITY; OSTEOGENIC CULTURE; OSTEOGENIC DIFFERENTIATION; OSTEOGENIC SUPPLEMENTS;

MINERALOGY;

ALKALINE PHOSPHATASE; ASCORBIC ACID; BONE SIALOPROTEIN; CALCIUM; COMPLEMENTARY DNA; DEXAMETHASONE; DOUBLE STRANDED DNA; OSTEOCALCIN; OSTEOPONTIN; RNA;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; BONE MINERALIZATION; CELL LINE; CELL STRAIN MC3T3 E1; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; DNA CONTENT; ENZYME ACTIVITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION;

ALKALINE PHOSPHATASE; ANIMALS; CALCIFICATION, PHYSIOLOGIC; CALCIUM; CELL CULTURE TECHNIQUES; CELL LINE; CELL SHAPE; CELL SURVIVAL; GENE EXPRESSION REGULATION; MICE; OSTEOBLASTS; OSTEOGENESIS;

EID: 84896895470     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2012.0412     Document Type: Article

References (23)

1. Sudo, H., Kodama, H.A., Amagai, Y., Yamamoto, S., and Kasai, S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 96, 191, 1983.
2. Owen, T.A., Aronow, M., Shalhoub, V., Barone, L.M., Wilming, L., Tassinari, M.S., et al. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143, 420, 1990.
3. Quarles, L.D., Yohay, D.A., Lever, L.W., Caton, R., and Wenstrup, R.J. Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res 7, 683, 1992.
4. Wang, D., Christensen, K., Chawla, K., Xiao, G., Krebsbach, P.H., and Franceschi, R.T. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 14, 893, 1999.
5. Kretlow, J.D., Jin, Y.Q., Liu, W., Zhang, W.J., Hong, T.H., Zhou, G., et al. Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol 9, 60, 2008.
6. Ter Brugge, P.J., and Jansen, J.A. In vitro osteogenic differentiation of rat bone marrow cells subcultured with and without dexamethasone. Tissue Eng 8, 321, 2002.
7. Wan Safwani, W.K., Makpol, S., Sathapan, S., and Chua, K.H. The changes of stemness biomarkers expression in human adipose-derived stem cells during long-term manipulation. Biotechnol Appl Biochem 58, 261, 2011.
8. Hughes, P., Marshall, D., Reid, Y., Parkes, H., and Gelber, C. The costs of using unauthenticated, over-passaged cell lines: how much more data do we need? Biotechniques 43, 575, 2007.
9. Chung, C.Y., Iida-Klein, A., Wyatt, L.E., Rudkin, G.H., Ishida, K., Yamaguchi, D.T., et al. Serial passage of MC3T3-E1 cells alters osteoblastic function and responsiveness to transforming growth factor-beta1 and bone morphogenetic protein-2. Biochem Biophys Res Commun 265, 246, 1999.
10. Peterson, W.J., Tachiki, K.H., and Yamaguchi, D.T. Serial passage of MC3T3-E1 cells down-regulates proliferation during osteogenesis in vitro. Cell Prolif 37, 325, 2004.
11. Song, I.H., Caplan, A.I., and Dennis, J.E. In vitro dexamethasone pretreatment enhances bone formation of human mesenchymal stem cells in vivo. J Orthop Res 27, 916, 2009.
12. Cheng, S.L., Yang, J.W., Rifas, L., Zhang, S.F., and Avioli, L.V. Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology 134, 277, 1994.
13. Mostafa, N.Z., Uludag, H., Varkey, M., Dederich, D.N., Doschak, M.R., and El-Bialy, T.H. In vitro osteogenic induction of human gingival fibroblasts for bone regeneration. Open Dent J 5, 139, 2011.
14. Roberts, S.J., Chen, Y., Moesen, M., Schrooten, J., and Luyten, F.P. Enhancement of osteogenic gene expression for the differentiation of human periosteal derived cells. Stem Cell Res 7, 137, 2011.
15. Jin, Z.L., Zhang, Y.K., Sun, H.Y., Lin, Z., Bi, Y.C., Duan, Y.Z., et al. Osteogenic-related gene expression profiles of human dental follicle cells induced by dexamethasone. Acta Pharmacol Sin 29, 1013, 2008.
16. Arriero, M.D., Ramis, J.M., Perello, J., and Monjo, M. Differential response of MC3T3-E1 and human mesenchymal stem cells to inositol hexakisphosphate. Cell Physiol Biochem 30, 974, 2012.
17. Livak, K.J., and Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402, 2001.
18. Kono, R., Okuno, Y., Inada, K., Tokuda, A., Hashizume, H., Yoshida, M., et al. A Prunus mume extract stimulated the proliferation and differentiation of osteoblastic MC3T3-E1 cells. Biosci Biotechnol Biochem 75, 1907, 2011.
19. Beck, G.R., Jr., Sullivan, E.C., Moran, E., and Zerler, B. Relationship between alkaline phosphatase levels, osteopontin expression, and mineralization in differentiating MC3T3-E1 osteoblasts. J Cell Biochem 68, 269, 1998.
20. van Steenbrugge, G.J., van Uffelen, C.J., Bolt, J., and Schroder, F.H. The human prostatic cancer cell line LNCaP and its derived sublines: an in vitro model for the study of androgen sensitivity. J Steroid Biochem Mol Biol 40, 207, 1991.
21. Pradel, W., Mai, R., Gedrange, T., and Lauer, G. Cell passage and composition of culture medium effects proliferation and differentiation of human osteoblast-like cells from facial bone. J Physiol Pharmacol 59 Suppl 5, 47, 2008.
22. Mostafa, N.Z., Fitzsimmons, R., Major, P.W., Adesida, A., Jomha, N., Jiang, H., et al. Osteogenic differentiation of human mesenchymal stem cells cultured with dexamethasone, vitamin d3, basic fibroblast growth factor, and bone morphogenetic protein-2. Connect Tissue Res 53, 117, 2012.
23. Aubin, J.E. Osteoprogenitor cell frequency in rat bone marrow stromal populations: role for heterotypic cell-cell interactions in osteoblast differentiation. J Cell Biochem 72, 396, 1999.