Biochemical and molecular evidence of a δ9 fatty acid desaturase from Ensifer meliloti 1021

Microbiological Research

Volumn 169, Issue 5-6, 2014, Pages 463-468

  Paulucci, Natalia S ^a   Dardanelli, Marta S ^a   García de Lema, Mirta ^a  

^a DEPARTAMENTO DE QUÍMICA   (Argentina)

Author keywords

Aerobic mechanism; Desaturase gene; Fatty acid composition; Unsaturated fatty acids; 9 Fatty acid desaturase

Indexed keywords

ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE EXPRESSION; MONOUNSATURATED FATTY ACIDS; STEARIC ACID; UNSATURATED FATTY ACIDS;

DESATURASE; DESATURATION; E. COLI; ENSIFER; FATTY ACID COMPOSITION; HIGH GROWTH TEMPERATURES; RHIZOBIAL STRAINS;

GENE ENCODING;

ENZYME; FATTY ACID; GENE; GROWTH RATE; PROTEIN; TEMPERATURE EFFECT;

ACYL COENZYME A DESATURASE; DELTA-9 FATTY ACID DESATURASE; FATTY ACID;

ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; SINORHIZOBIUM MELILOTI;

CLONING, MOLECULAR; ESCHERICHIA COLI; FATTY ACID DESATURASES; FATTY ACIDS; GENE EXPRESSION; SINORHIZOBIUM MELILOTI;

EID: 84896513311     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2013.08.003     Document Type: Article

References (28)

1. Aguilar P., de Mendoza D. Control of fatty acid desaturation: a mechanism conserved from bacteria to humans. Mol Microbiol 2006, 62:1507-1514.
2. Beringer J. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 1974, 84:188-198.
3. Bligh E., Dyer W. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959, 37:911-918.
4. Cao Y., Xian M., Yang J., Xu X., Liu W., Li L. Heterologous expression of stearoyl-acyl carrier protein desaturase (S-ACP-DES) from Arabidopsis thaliana in Escherichia coli. Protein Expr Purif 2010, 69:209-214.
5. Chung C., Niemela S., Miller R. One-step preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci U S A 1989, 86:2172-2175.
6. Cronan J., Rock C. Biosynthesis of membrane lipids. Escherichia coli and Salmonella: Cellular and Molecular Biology 1996, 612-636. ASM Press, Washington, DC. F.C. Neidhardt, R. Curtis, J.L. Ingraham, E.C. Lin, K.B. Low, B. Magasanik, W.S. Reznikoff, M. Riley, M. Schaechter, H.E. Umbarger (Eds.).
7. Davanloo P., Rosenberg A., Dunn J., Studier F. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A 1984, 81:2035-2039.
8. Henderson R., Tocher D. Thin layer chromatography. Lipid Analysis a Practical Approach 1992, 65-111. Oxford University Press, Oxford/New York/Tokyo. R. Hamilton, S. Hamilton (Eds.).
9. Kates M. Radioisotopic techniques in lipidology. Techniques in Lipidology 1972, 498-500. North Holland/Elsevier, Amsterdam/New York. T.S. Work, E. Work (Eds.).
10. Li Y., Xu X., Dietrich M., Urlacher V., Schmid R., Ouyang P., He B. Identification and functional expression of a δ9 fatty acid desaturase from the marine bacterium Pseudoalteromonas sp. MLY15. J Mol Catal B Enzym 2009, 56:96-101.
11. López-Alonso D., García-Maroto F., Rodríguez-Ruiz J., Garrido J., Vilches M. Evolution of the membrane-bound fatty acid desaturases. Biochem Syst Ecol 2003, 31:1111-1124.
12. Lopez-Lara I., Geiger O. Expression and purification of four different rhizobial acyl carrier proteins. Microbiology 2000, 146:839-849.
13. Los D., Murata N. Structure and expression of fatty acid desaturase. Biochim Biophys Acta 1998, 1394:3-15.
14. Macartney A., Maresca B., Cossins A. Acyl-CoA desaturases and the adaptive regulation of membrane lipid composition. Temperature Adaptation of Biological Membranes 1994, 129-139. Portland Press, London. A.R. Cossins (Ed.).
15. Magnuson K., Jackowski S., Rock C., Cronan J. Regulation of fatty acid biosynthesis in escherichia coli. Microbiol Rev 1993, 57:522-542.
16. Mansilla M., Cybulski L., Albanesi D., de Mendoza D. Control of membrane lipid fluidity by molecular thermosensors. J Bacteriol 2004, 186:6681-6688.
17. Meade H., Long S., Ruvkun G., Brown S., Ausubel F. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol 1982, 149:114-122.
18. Morrison W., Smith L. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride. J Lipid Res 1964, 5:600-608.
19. Nishida I., Murata N. Chilling sensitivity in plants and cyanobacteria: the crucial contribution of membrane lipids. Annu Rev Plant Physiol Plant Mol Biol 1996, 47:541-568.
20. Paulucci N., Medeot D., Dardanelli M., García de Lema M. Growth temperature and salinity impact fatty acid composition and degree of unsaturation in peanut-nodulating rhizobia. Lipids 2011, 46:435-441.
21. Paulucci N., Medeot D., Woelke M., Dardanelli M., García de Lema M. Monounsaturated fatty acid aerobic synthesis in Bradyrhizobium TAL1000 peanut-nodulating is affected by temperature. J Appl Microbiol 2013, 114:1457-1467.
22. Phetsuksiri B., Jackson M., Scherman H., McNeil M., Besra G., Baulard A.R., et al. Unique mechanism of action of the thiourea drug isoxyl on Mycobacterium tuberculosis. J Biol Chem 2003, 278:53123-53130.
23. Robertsen B.K., Aiman P., Darwill A.G., McNeil M.y., Albersheim P. The structure of acidic extracellular polysaccharides secreted by Rhizobium leguminosarum and Rhizobium trifoli. Plant Physiol 1981, 67:389-400.
24. Shanklin J., Cahoon E. Desaturation and related modifications of fatty acids. Plant Physiol 1998, 49:611-641.
25. Shivaji S., Prakash S. How do bacteria sense and respond to low temperature?. Arch Microbiol 2010, 192:85-95.
26. Tighe S., de Lajudie P., Dipietro K., Lindström K., Nick G., Jarvis B. Analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the Sherlock microbial identification system. Int J Syst Evol Microbiol 2000, 50:787-801.
27. Wada M., Fukunaga N., Sasaki S. Mechanism of biosynthesis of unsaturated fatty acids in Pseudomonas sp. strain E-3, a psychrotrophic bacterium. J Bacteriol 1989, 171:4267-4271.
28. Zhu K., Kyoung-Hee C., Schweizer H., Rock C., Zhang Y. Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa. Mol Microbiol 2006, 60:260-273.