Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors

Biotechnology Letters

Volumn 36, Issue 4, 2014, Pages 843-853

  Jaremicz, Zbigniew ^a   Luczkiewicz, Maria ^a   Kokotkiewicz, Adam ^a   Krolicka, Aleksandra ^a   Sowinski, Pawel ^b  

^a MEDICAL UNIVERSITY OF GDANSK   (Poland)

^b GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Black henbane; Bubble bioreactors; Hairy roots; Hyoscyamus niger; Methyl jasmonate (elicitor); Scopolamine; Spray bioreactor; Tropane alkaloids

Indexed keywords

ALKALOIDS; BIOREACTORS; BUBBLE COLUMNS; PRODUCTIVITY;

BLACK HENBANE; BUBBLE BIOREACTORS; HAIRY ROOTS; HYOSCYAMUS NIGER; METHYL JASMONATE; SCOPOLAMINE; TROPANE ALKALOIDS;

BIOCONVERSION;

HYOSCYAMUS; HYOSCYAMUS NIGER;

ALKALOID; CYCLOPENTANE DERIVATIVE; JASMONIC ACID; OXYLIPIN; TROPANE DERIVATIVE;

ARTICLE; BIOREACTOR; HYOSCYAMUS; ISOLATION AND PURIFICATION; METABOLISM; PLANT ROOT;

ALKALOIDS; BIOREACTORS; CYCLOPENTANES; HYOSCYAMUS; OXYLIPINS; PLANT ROOTS; TROPANES;

EID: 84896392945     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1426-9     Document Type: Article

References (18)

1. Baranski R, Klocke E, Schumann G (2006) Green fluorescent protein as an efficient selection marker for Agrobacterium rhizogenes mediated carrot transformation. Plant Cell Rep 25: 190-197.
2. Cusido RM, Palazón J, Piñol MT, Bonfill M, Morales C (1999) Datura metel: in vitro production of tropane alkaloids. Planta Med 65: 144-148.
3. Georgiev MI, Agostini E, Ludwig-Müller J, Xu J (2012) Genetically transformed roots: from plant disease to biotechnological resource. Trends Biotechnol 30: 528-537.
4. Grynkiewicz G, Gadzikowska M (2008) Tropane alkaloids as medicinally useful natural products and their synthetic derivatives as new drugs. Pharmacol Rep 60: 439-463.
5. Jaremicz Z, Luczkiewicz M, Kisiel M, Zárate R, El Jaber-Vazdekis N, Migas P (2013) Multi-development-HPTLC method for quantitation of hyoscyamine, scopolamine and their biosynthetic precursors in selected Solanaceae plants grown in natural conditions and as in vitro cultures. Phytochem Anal. doi: 10. 1002/pca. 2455.
6. Kim Y, Wyslouzil BE, Weathers PJ (2002) Secondary metabolism of hairy root cultures in bioreactors. In Vitro Cell Dev Biol Plant 38: 1-10.
7. Krolicka A, Szpitter A, Stawujak K, Baranski R, Gwizdek-Wisniewska A, Skrzypczak A, Kaminski M, Lojkowska E (2010) Teratomas of Drosera capensis var. alba as a source of naphthoquinone: ramentaceone. Plant Cell Tissue Organ Cult 103: 285-292.
8. Kuźma Ł, Bruchajzer E, Wysokińska H (2009) Methyl jasmonate effect on diterpenoid accumulation in Salvia sclarea hairy root culture in shake flasks and sprinkle bioreactor. Enzyme Microb Technol 44: 406-410.
9. Li J, van Belkum MJ, Vederas JC (2012) Functional characterization of recombinant hyoscyamine 6β-hydroxylase from Atropa belladonna. Bioorg Med Chem 20: 4356-4363.
10. Luczkiewicz M, Kokotkiewicz A (2012) Elicitation and permeabilisation affect the accumulation and storage profile of phytoestrogens in high productive suspension cultures of Genista tinctoria. Acta Physiol Plant 34: 1-16.
11. Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT, Choi MS (2007) Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresour Technol 98: 1748-1753.
12. Palazón J, Navarro-Ocaña A, Hernandez-Vazquez L, Mirjalili MH (2008) Application of metabolic engineering to the production of scopolamine. Molecules 13: 1722-1742.
13. Srivastava S, Srivastava AK (2007) Hairy root culture for mass-production of high-value secondary metabolites. Crit Rev Biotechnol 27: 29-43.
14. Uranbey S (2005) Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger. Biol Plant 49: 427-430.
15. Wilhelmson A, Häkkinen ST, Kallio PT, Oksman-Caldentey K-M, Nuutila AM (2006) Heterologous expression of Vitreoscilla hemoglobin (VHb) and cultivation conditions affect the alkaloid profile of Hyoscyamus muticus hairy roots. Biotechnol Prog 22: 350-358.
16. Williams GRC, Doran PM (2000) Hairy root culture in a liquid-dispersed bioreactor: characterization of spatial heterogeneity. Biotechnol Prog 16: 391-401.
17. Woo SH, Park JM, Yang J-W (1996) Root culture using a mist culture system and estimation of scale-up feasibility. J Chem Technol Biotechnol 66: 355-362.
18. Zhang L, Yang B, Lu B, Kai G, Wang Z, Xia Y, Ding R, Zhang H, Sun X, Chen W, Tang K (2007) Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent. Planta 225: 887-896.