The depletion of F1 subunit ε in yeast leads to an uncoupled respiratory phenotype that is rescued by mutations in the proton-translocating subunits of F0

Molecular Biology of the Cell

Volumn 25, Issue 6, 2014, Pages 791-799

  Tetaud, Emmanuel ^a,b   Godard, François ^a,b   Giraud, Marie France ^a,b   Ackerman, Sharon H ^c   Di Rago, Jean Paul ^a,b  

^a CNRS   (France)

^b UNIVERSITÉ DE BORDEAUX   (France)

^c WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; FUNGAL PROTEIN; MITOCHONDRIAL DNA; MITOCHONDRIAL ENZYME; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE;

ARTICLE; ATP15 GENE; CELL DENSITY; DNA SEQUENCE; DOWN REGULATION; FUNGUS MUTATION; GENE; GENE EXPRESSION; GENETIC STABILITY; MISSENSE MUTATION; MITOCHONDRIAL RESPIRATION; NONHUMAN; NULL ALLELE; OXIDATIVE PHOSPHORYLATION; OXIDATIVE PHOSPHORYLATION UNCOUPLING; OXYGEN CONSUMPTION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN SUBUNIT; PROTON TRANSPORT; RESPIRATORY CHAIN; WESTERN BLOTTING;

CELL ENGINEERING; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; GENE EXPRESSION; GENOTYPE; MITOCHONDRIA; MUTATION; OXIDATIVE PHOSPHORYLATION; PHENOTYPE; PROTEIN SUBUNITS; PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; THERMODYNAMICS;

EID: 84896297546     PISSN: 10591524     EISSN: 19394586     Source Type: Journal    
DOI: 10.1091/mbc.E13-02-0112     Document Type: Article

References (23)

1. Arselin G, Vaillier J, Graves PV, Velours J (1996). ATP synthase of yeast mitochondria. Isolation of the subunit h and disruption of the ATP14 gene. J Biol Chem 271, 20284-20290. (Pubitemid 26281793)
2. Dautant A, Velours J, Giraud MF (2010). Crystal structure of the Mg.ADP-inhibited state of the yeast F1c10 ATP synthase. J Biol Chem 285, 29502-29510.
3. Devenish RJ, Prescott M, Rodgers AJ (2008). The structure and function of mitochondrial F1F0-ATP synthases. Int Rev Cell Mol Biol 267, 1-58.
4. Duvezin-Caubet S, Caron M, Giraud MF, Velours J, di Rago JP (2003). The two rotor components of yeast mitochondrial ATP synthase are mechanically coupled by subunit delta. Proc Natl Acad Sci USA 100, 13235-13240. (Pubitemid 37444725)
5. Duvezin-Caubet S, Rak M, Lefebvre-Legendre L, Tetaud E, Bonnefoy N, di Rago JP (2006). A "petite obligate" mutant of Saccharomyces cerevisiae: functional mtDNA is lethal in cells lacking the delta subunit of mitochondrial F1-ATPase. J Biol Chem 281, 16305-16313.
6. Emaus RK, Grunwald R, Lemasters JJ (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochim Biophys Acta 850, 436-448. (Pubitemid 16077550)
7. Giraud MF, Velours J (1997). The absence of the mitochondrial ATP synthase delta subunit promotes a slow growth phenotype of rho-yeast cells by a lack of assembly of the catalytic sector F1. Eur J Biochem 245, 813-818.
8. Godard F, Tetaud E, Duvezin-Caubet S, di Rago JP (2011). A genetic screen targeted on the FO component of mitochondrial ATP synthase in Saccharomyces cerevisiae. J Biol Chem 286, 18181-18189.
9. Grandier-Vazeille X, Guerin M (1996). Separation by blue native and colorless native polyacrylamide gel electrophoresis of the oxidative phosphorylation complexes of yeast mitochondria solubilized by different detergents: specific staining of the different complexes. Anal Biochem 242, 248-254. (Pubitemid 26386821)
10. Guelin E, Chevallier J, Rigoulet M, Guerin B, Velours J (1993). ATP synthase of yeast mitochondria. Isolation and disruption of the ATP epsilon gene. J Biol Chem 268, 161-167. (Pubitemid 23021299)
11. Guerin B, Labbe P, Somlo M (1979). Preparation of yeast mitochondria (Saccharomyces cerevisiae) with good P/O and respiratory control ratios. Methods Enzymol 55, 149-159.
12. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
13. Lai-Zhang J, Xiao Y, Mueller DM (1999). Epistatic interactions of deletion mutants in the genes encoding the F1-ATPase in yeast Saccharomyces cerevisiae. EMBO J 18, 58-64. (Pubitemid 29005023)
14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-275.
15. Paul MF, Ackerman S, Yue J, Arselin G, Velours J, Tzagolof A, Ackermann S (1994). Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants. J Biol Chem 269, 26158-26164. (Pubitemid 24328426)
16. Rigoulet M, Guerin B (1979). Phosphate transport and ATP synthesis in yeast mitochondria: effect of a new inhibitor: the tribenzylphosphate. FEBS Lett 102, 18-22.
17. Schagger H, von Jagow G (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166, 368-379. (Pubitemid 18004907)
18. Schagger H, von Jagow G (1991). Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199, 223-231.
19. Somlo M (1968). Induction and repression of mitochondrial ATPase in yeast. Eur J Biochem 52, 276-284.
20. Stock D, Leslie AG, Walker JE (1999). Molecular architecture of the rotary motor in ATP synthase. Science 286, 1700-1705. (Pubitemid 129515869)
21. Wach A, Brachat A, Pohlmann R, Philippsen P (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
22. Walker JE (2013). The ATP synthase: the understood, the uncertain and the unknown. Biochem Soc Trans 41, 1-16.
23. Xiao Y, Metzl M, Mueller DM (2000). Partial uncoupling of the mitochondrial membrane by a heterozygous null mutation in the gene encoding the gamma- or delta-subunit of the yeast mitochondrial ATPase. J Biol Chem 275, 6963-6968. (Pubitemid 30146239)