Western Blot: Protein transfer overview

North American Journal of Medical Sciences

Volumn 6, Issue 3, 2014, Pages 158-159

  Gibbons, Janay ^a  

^a ANGLIA RUSKIN UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

POLYVINYLIDENE FLUORIDE; PYROXYLIN;

DIFFUSION; ELECTRIC SHOCK; ELUTION; HAZARD; LETTER; MEMBRANE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BLOTTING; WESTERN BLOTTING;

EID: 84896029977     PISSN: 22501541     EISSN: 19472714     Source Type: Journal    
DOI: 10.4103/1947-2714.128481     Document Type: Letter

References (9)

1. Mahmood T, Yang PC. Western Blot: Technique, theory, and trouble shooting. N Am J Med Sci 2012;4:429-34.
2. Kurien BT, Scofield RH. Western blotting. Methods 2006;38:283-93.
3. MacPhee DJ. Methodological considerations for improving Western blot analysis. J Pharmacol Toxicol Methods 2010;61:171-7.
4. Kurien BT, Scofield RH. Protein blotting: A review. J Immunol Methods 2003;274:1-15.
5. Kurien BT, Scofield RH. Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens. J Immunol Methods 1997;205:91-4.
6. The iBlot® Dry Blotting System vs. Conventional Semi-Dry and Wet Transfer Systems. Paisley, UK. Life Technologies, 2013. (Accessed December 17, 2013, at http://www. lifetechnologies.com/uk/en/home/life-science/proteinexpression-and-analysis/western-blotting/western-blottransfer/ iblot-dry-blottingsystem/iblot-dry-blottingcomparison-to-semi-dry-and-wet.html).
7. Kurien B, Scofield R. Introduction to Protein Blotting. In: Kurien B, Scofi eld R (Eds.), Protein blotting and detection. New York: Humana Press 2009;3:9-21.
8. Wisdom GB. Protein blotting. Methods Mol Biol 1994;32:207-13.
9. Kurien BT, Scofield RH. Heat-mediated, ultra-rapid electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes. J Immunol Methods 2002;266:127-33.