Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation

Analytical Biochemistry

Volumn 451, Issue 1, 2014, Pages 25-27

  Ebersole, Brittany ^a   Petko, Jessica ^a   Levenson, Robert ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

Click chemistry; Immunoprecipitation; Mu opioid receptor; Palmitoylation

Indexed keywords

PROTEINS; SYNTHESIS (CHEMICAL);

CELLULAR PROTEINS; CHEMICAL PROBES; CLICK CHEMISTRY; G-PROTEIN COUPLED RECEPTORS; IMMUNOPRECIPITATIONS; MAGNETIC BEADS; OPIOID RECEPTORS; PALMITOYLATION;

ACYLATION;

15 HEXADECYNOIC ACID; MU OPIATE RECEPTOR; UNCLASSIFIED DRUG; UNSATURATED FATTY ACID;

ARTICLE; CELL LYSATE; CELL STRAIN HEK293; CLICK CHEMISTRY; EMBRYO; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOPRECIPITATION; PALMITOYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS;

CLICK CHEMISTRY; IMMUNOPRECIPITATION; MU-OPIOID RECEPTOR; PALMITOYLATION;

ANTIBODIES; CLICK CHEMISTRY; FATTY ACIDS, UNSATURATED; HEK293 CELLS; HUMANS; IMMUNOBLOTTING; IMMUNOMAGNETIC SEPARATION; IMMUNOPRECIPITATION; LIPOYLATION; RECEPTORS, OPIOID, MU; RECOMBINANT FUSION PROTEINS;

EID: 84895547242     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.01.008     Document Type: Article

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