Detection of intestinal protozoa in the clinical laboratory

Journal of Clinical Microbiology

Volumn 52, Issue 3, 2014, Pages 712-720

  McHardy, Ian H ^a   Wu, Max ^a   Shimizu Cohen, Robyn ^a   Roger Couturier, Marc ^b,c   Humphries, Romney M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b ARUP INSTITUTE FOR CLINICAL AND EXPERIMENTAL PATHOLOGY   (United States)

^c UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COPPER DERIVATIVE; FIXATIVE; MERCURIC CHLORIDE; MERCURY DERIVATIVE; POLYVINYL ALCOHOL; PRESERVATIVE; RNA 18S; ZINC DERIVATIVE;

AMEBIASIS; BALANTIDIASIS; BALANTIDIUM; BALANTIDIUM COLI; BLASTOCYSTIS HOMINIS; CLINICAL LABORATORY; CRYPTOSPORIDIUM; CYCLOSPORA CAYETANENSIS; DEVELOPED COUNTRY; DIAGNOSTIC EQUIPMENT; DIAGNOSTIC PROCEDURE; DIAGNOSTIC TEST APPROVAL; DIENTAMOEBA; DIENTAMOEBIASIS; ENDEMIC SPECIES; ENDOLIMAX NANA; ENTAMOEBA HISTOLYTICA; ENZYME IMMUNOASSAY; FECES ANALYSIS; FLUORESCENCE MICROSCOPY; FOOD AND DRUG ADMINISTRATION; GIARDIA LAMBLIA; GIARDIASIS; HISTOLOGY; HUMAN; IMMUNOAFFINITY CHROMATOGRAPHY; INTESTINE PARASITE; ISOSPORA BELLI; ISOSPORIASIS; MEDICAL TECHNOLOGIST; MEDICAL TECHNOLOGY; MICROSCOPY; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PARASITE IDENTIFICATION; PARASITE VIRULENCE; PATHOGENESIS; PRESERVATION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTOZOON; REVIEW; RNA GENE; SENSITIVITY AND SPECIFICITY; SEROLOGY; SPECIMEN COLLECTION KITS; TROPHOZOITE;

CLINICAL LABORATORY TECHNIQUES; CRYPTOSPORIDIUM; CYCLOSPORA; DIAGNOSTIC TESTS, ROUTINE; DIENTAMOEBA; ENTAMOEBA HISTOLYTICA; GIARDIA LAMBLIA; HUMANS; INTESTINAL DISEASES, PARASITIC; PARASITOLOGY; PROTOZOAN INFECTIONS;

EID: 84894552763     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02877-13     Document Type: Review

References (36)

1. Fletcher SM, Stark D, Harkness J, Ellis J. 2012. Enteric protozoa in the developed world: a public health perspective. Clin. Microbiol. Rev. 25: 420-449. http://dx.doi.org/10.1128/CMR.05038-11.
2. Centers for Disease Control and Prevention. 2013. Notes from the field: use of electronic messaging and the news media to increase case finding during a Cyclospora outbreak-Iowa, July 2013. MMWR Morb. Mortal. Wkly. Rep. 62:613-614. http://www.cdc.gov/mmwr/preview/mmwrhtml.mm6230a4.htm.
3. Polage CR, Stoddard GJ, Rolfs RT, Petti CA. 2011. Physician use of parasite tests in the United States from 1997 to 2006 and in a Utah Cryptosporidium outbreak in 2007. J. Clin. Microbiol. 49:591-596. http://dx.doi.org/10.1128/JCM.01806-10.
4. Branda JA, Lin TY, Rosenberg ES, Halpern EF, Ferraro MJ. 2006. A rational approach to the stool ova and parasite examination. Clin. Infect. Dis. 42:972-978. http://dx.doi.org/10.1086/500937.
5. Bruijnesteijn van Coppenraet LE, Wallinga JA, Ruijs GJ, Bruins MJ, Verweij JJ. 2009. Parasitological diagnosis combining an internally controlled real-time PCR assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy. Clin. Microbiol. Infect. 15:869-874. http://dx.doi.org/10.1111/j.1469-0691.2009.02894.x.
6. Garcia LS. 2009. Practical guide to diagnostic parasitology. ASM Press, Washington, DC.
7. Nazer H, Greer W, Donnelly K, Mohamed AE, Yaish H, Kagalwalla A, Pavillard R. 1993. The need for three stool specimens in routine laboratory examinations for intestinal parasites. Br. J. Clin. Pract. 47:76-78. (Pubitemid 23137768)
8. Hiatt RA, Markell EK, Ng E. 1995. How many stool examinations are necessary to detect pathogenic intestinal protozoa? Am. J. Trop. Med. Hyg. 53:36-39.
9. Pietrzak-Johnston SM, Bishop H, Wahlquist S, Moura H, Da Silva ND, Nguyen-Dihn P. 2000. Evaluation of commercially available preservatives for laboratory detection of helminths and protozoa in human fecal specimens. J. Clin. Microbiol. 38:1959-1964. http://dx.doi.org/10.1128/JCM.38.5.1959-1964. 2000. (Pubitemid 30253944)
10. Garcia LS, Shimizu RY, Brewer TC, Bruckner DA. 1983. Evaluation of intestinal parasite morphology in polyvinyl alcohol preservative: comparison of copper sulfate and mercuric chloride bases for use in Schaudinn fixative. J. Clin. Microbiol. 17:1092-1095. (Pubitemid 13098873)
11. Garcia LS, Shimizu RY, Shum A, Bruckner DA. 1993. Evaluation of intestinal protozoan morphology in polyvinyl alcohol preservative: comparison of zinc sulfate-and mercuric chloride-based compounds for use in Schaudinn's fixative. J. Clin. Microbiol. 31:307-310. (Pubitemid 23028630)
12. Garcia LS, Shimizu RY. 1998. Evaluation of intestinal protozoan morphology in human fecal specimens preserved in EcoFix: comparison of Wheatley's trichrome stain and EcoStain. J. Clin. Microbiol. 36:1974-1976. (Pubitemid 28277787)
13. Feng Y, Xiao L. 2011. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin. Microbiol. Rev. 24:110-140. http://dx.doi.org/10.1128/CMR.00033-10.
14. Clinical and Laboratories Standards Institute. 1997. Procedures for the recovery and identification of parasites from the intestinal tract; approved guideline, 2nd ed. CLSI document M28-A2. Clinical and Laboratories Standards Institute, Wayne, PA.
15. Alles AJ, Waldron MA, Sierra LS, Mattia AR. 1995. Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens. J. Clin. Microbiol. 33:1632-1634.
16. Maraha B, Buiting AG. 2000. Evaluation of four enzyme immunoassays for the detection of Giardia lamblia antigen in stool specimens. Eur. J. Clin. Microbiol. Infect. Dis. 19:485-487. http://dx.doi.org/10.1007/s1009600000286. (Pubitemid 30458663)
17. Garcia LS, Shimizu RY. 1997. Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J. Clin. Microbiol. 35:1526-1529. (Pubitemid 27216825)
18. Johnson EH, Windsor JJ, Clark CG. 2004. Emerging from obscurity: biological, clinical, and diagnostic aspects of Dientamoeba fragilis. Clin. Microbiol. Rev. 17:553-570. http://dx.doi.org/10.1128/CMR.17.3.55-570.2004. (Pubitemid 38970682)
19. Barratt JL, Harkness J, Marriott D, Ellis JT, Stard D. 2011. A review of Dientamoeba fragilis carriage in humasn: several reasons why this organism shoul be condired in the diagnosis of gastrointestinal illness. Gut Microbes 2:3-12. http://dx.doi.org/10.4161/gmic.2.1.14755.
20. Munasinghe VS, Vella NG, Ellis JT, Windsor PA, Stark D. 2013. Cyst formation and faecal-oral transmission of Dientamoeba fragilis: the missing link in the life cycle of an emerging pathogen. Int. J. Parasitol. 43:879-883. http://dx.doi.org/10.1016/j.ijpara.2013.06.003.
21. Stark D, Barratt J, Roberts T, Marriott D, Harkness J, Ellis J. 2010. Comparison of microscopy, two xenic culture techniques, conventional and real-time PCR for the detection of Dientamoeba fragilis in clinical stool samples. Eur. J. Clin. Microbiol. Infect. Dis. 29:411-416. http://dx.doi.org/10. 1007/s10096-010-0867-4.
22. Agnamey P, Sarfati C, Pinel C, Rabodoniriina M, Kapel N, Dutoit E, Garnaud C, Diouf M, Garin JF, Totet A, Derouin F, Cryptosporidium National Network ANOFEL. 2011. Evaluation of four commercial rapid immunochromatographic assays for detection of Cryptosporidium antigens in stool samples: a blind multicenter trial. J. Clin. Microbiol. 49: 1605-1607. http://dx.doi.org/10.1128/ JCM.02074-10.
23. Visvesvara GS, Moura H, Kovacs-Nace E, Wallace S, Eberhard ML. 1997. Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J. Clin. Microbiol. 35: 730-733. (Pubitemid 27083870)
24. Relman DA, Schmidt TM, Gajadhar A, Sogin M, Cross J, Yoder K, Sethabutr O, Echeverria P. 1996. Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species. J. Infect. Dis. 173:440-445. (Pubitemid 26042446)
25. Lindsay DS, Dubey JP, Blagburn BL. 1997. Biology of Isospora spp. from humans, nonhuman primates, and domestic animals. Clin. Microbiol. Rev. 10:19-34. (Pubitemid 27042830)
26. DPDx. 2009. Key points for laboratory diagnosis of cystoisosporiasis. Centers for Disease Control and Prevention, Atlanta, GA. http://dpd.cdc.gov/ dpdx/HTML/Cystoisosporiasis.htm.
27. Cuomo MJN, Lawrence B, White DB. 2012. Diagnosing medical parasites: a public health officers guide to assisting laboratory and medical officers. USAF Air Education and Training Command, Randolph, TX. http://www.phsource.us/PH/ PARA/.
28. WHO/PAHO/UNESCO. 1997. WHO/PAHO/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico, 28-29 January, 1997. Epidemiol. Bull. 18:13-14.
29. Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J. 2007. Laboratory diagnostic techniques for Entamoeba species. Clin. Microbiol. Rev. 20:511-532. http://dx.doi.org/10.1128/CMR.00004-07. (Pubitemid 47175017)
30. Blumencranz H, Kasen L, Romeu J, Waye JD, LeLeiko NS. 1983. The role of endoscopy in suspected amebiasis. Am. J. Gastroenterol. 78:15-18. (Pubitemid 13181044)
31. Abe T, Kawai N, Yasumaru M, Mizutani M, Akamatsu H, Fujita S, Nishida T, Iijima H, Tsujuii M, Tsujimoto M. 2009. Ameboma mimicking colon cancer. Gastrointest. Endosc. 69:757-758. http://dx.doi.org/10.1015/j.gie.2008.12.065.
32. Upadhyay R, Gupta N, Gogia P, Chandra S. 2012. Poached egg appearance in intestinal amebiasis. Gastrointest. Endosc. 76:189-190. http://dx.doi.org/10. 1016/j.gie.2012.03.171.
33. Tan KS. 2008. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin. Microbiol. Rev. 21:639-665. http://dx.doi.org/10.1128/CMR.00022-08.
34. Schuster FL, Ramirez-Avila L. 2008. Current world status of Balantidium coli. Clin. Microbiol. Rev. 21:626-638. http://dx.doi.org/10.1128/CMR.00021-08.
35. Stark D, Al-Qassab SE, Barratt JL, Stanley K, Roberts T, Marriott D, Harkness J, Ellis JT. 2011. Evaluation of multiplex tandem real-time PCR for detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in clinical stool samples. J. Clin. Microbiol. 49:257-262. http://dx.doi.org/10.1128/JCM.01796-10.
36. Navidad JF, Griswold DJ, Gradus MS, Bhattacharyya S. 2013. Evaluation of Luminex xTag gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. J. Clin. Microbiol. 51: 3018-3024. http://dx.doi.org/10.1128/JCM.00896-13.