Fluorescent fatty acid analogs as a tool to study development of the picornavirus replication organelles

Journal of Virological Methods

Volumn 200, Issue , 2014, Pages 15-21

  Viktorova, Ekaterina G ^a   Ford Siltz, Lauren A ^a   Nchoutmboube, Jules ^a   Belov, George A ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

Lipid metabolism; Long chain fatty acid import; Phosphatidylcholine synthesis; Picornaviruses; Replication organelles

Indexed keywords

LIPID METABOLISM; LONG CHAIN FATTY ACID IMPORT; PHOSPHATIDYLCHOLINE SYNTHESIS; PICORNAVIRUSES; REPLICATION ORGANELLES;

FATTY ACIDS; FLUORESCENT DYES; HELA CELLS; HUMANS; OPTICAL IMAGING; ORGANELLES; PICORNAVIRIDAE; STAINING AND LABELING; VIROLOGY; VIRUS REPLICATION;

EID: 84894234200     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.01.020     Document Type: Article

References (21)

1. Belov G.A., Nair V., Hansen B.T., Hoyt F.H., Fischer E.R., Ehrenfeld E. Complex dynamic development of poliovirus membranous replication complexes. J. Virol. 2012, 86:302-312.
2. Belov G.A., van Kuppeveld F.J. (+)RNA viruses rewire cellular pathways to build replication organelles. Curr. Opin. Virol. 2012, 2:740-747.
3. Caffaro C.E., Boothroyd J.C. Evidence for host cells as the major contributor of lipids in the intravacuolar network of Toxoplasma-infected cells. Eukaryot. Cell 2011, 10:1095-1099.
4. Caliguiri L.A., Tamm I. The role of cytoplasmic membranes in poliovirus biosynthesis. Virology 1970, 42:100-111.
5. Egger D., Pasamontes L., Bolten R., Boyko V., Bienz K. Reversible dissociation of the poliovirus replication complex: functions and interactions of its components in viral RNA synthesis. J. Virol. 1996, 70:8675-8683.
6. Ehrenfeld E., Domingo E., Roos R.P., American Society for Microbiology The Picornaviruses 2010, ASM Press, Washington, DC.
7. Folch J., Lees M., Sloane Stanley G.H. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 1957, 226:497-509.
8. Hsu N.Y., Ilnytska O., Belov G., Santiana M., Chen Y.H., Takvorian P.M., Pau C., van der Schaar H., Kaushik-Basu N., Balla T., Cameron C.E., Ehrenfeld E., van Kuppeveld F.J., Altan-Bonnet N. Viral reorganization of the secretory pathway generates distinct organelles for RNA replication. Cell 2010, 141:799-811.
9. Kasurinen J. A novel fluorescent fatty-acid, 5-methyl-BDY-3-dodecanoic acid, is a potential probe in lipid transport studies by incorporating selectively to lipid classes of BHK cells. Biochem. Biophys. Res. Commun. 1992, 187:1594-1601.
10. Li Z., Vance D.E. Phosphatidylcholine and choline homeostasis. J. Lipid Res. 2008, 49:1187-1194.
11. Liao J.F., Sportsman R., Harris J., Stahl A. Real-time quantification of fatty acid uptake using a novel fluorescence assay. J. Lipid Res. 2005, 46:597-602.
12. Mashek D.G., Li L.O., Coleman R.A. Long-chain acyl-CoA synthetases and fatty acid channeling. Future Lipidol. 2007, 2:465-476.
13. Nchoutmboube J.A., Viktorova E.G., Scott A.J., Ford L.A., Pei Z., Watkins P.A., Ernst R.K., Belov G.A. Increased long chain acyl-Coa synthetase activity and fatty acid import is linked to membrane synthesis for development of picornavirus replication organelles. PLoS Pathog. 2013, 9:e1003401.
14. Rogers R.A., Jack R.M., Furlong S.T. Lipid and membrane-protein transfer from human neutrophils to schistosomes is mediated by ligand-binding (Vol 106, Pg 485, 1993). J. Cell Sci. 1994, 107:U2.
15. Rust R.C., Landmann L., Gosert R., Tang B.L., Hong W., Hauri H.P., Egger D., Bienz K. Cellular COPII proteins are involved in production of the vesicles that form the poliovirus replication complex. J. Virol. 2001, 75:9808-9818.
16. Spence M.T.Z., Johnson I.D. The Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies 2010, Live Technologies Corporation, Carlsbad, CA. 11th ed.
17. Suhy D.A., Giddings T.H., Kirkegaard K. Remodeling the endoplasmic reticulum by poliovirus infection and by individual viral proteins: an autophagy-like origin for virus-induced vesicles. J. Virol. 2000, 74:8953-8965.
18. van Meer G., Voelker D.R., Feigenson G.W. Membrane lipids: where they are and how they behave. Nat. Rev. Mol. Cell Biol. 2008, 9:112-124.
19. Vance D.E., Trip E.M., Paddon H.B. Poliovirus increases phosphatidylcholine biosynthesis in HeLa cells by stimulation of the rate-limiting reaction catalyzed by CTP: phosphocholine cytidylyltransferase. J. Biol. Chem. 1980, 255:1064-1069.
20. Vance J.E., Vance D.E. Phospholipid biosynthesis in mammalian cells. Biochem. Cell Biol. 2004, 82:113-128.
21. Wendel A.A., Lewin T.M., Coleman R.A. Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis. Biochim. Biophys. Acta 2009, 1791:501-506.