NADH changes during hypoxia, ischemia, and increased work differ between isolated heart preparations

American Journal of Physiology - Heart and Circulatory Physiology

Volumn 306, Issue 4, 2014, Pages

  Wengrowski, Anastasia M ^a   Kuzmiak Glancy, Sarah ^a   Jaimes III, Rafael ^a   Kay, Matthew W ^a,b  

^a The George Washington University   (United States)

^b GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

Biventricular working; Blebbistatin; Langendorff; Nadh imaging; Workload

Indexed keywords

BIVENTRICULAR WORKING; BLEBBISTATIN; LANGENDORFF; NADH IMAGING; WORKLOAD;

ANIMALS; ANOXIA; HEART; HEART RATE; MYOCARDIAL ISCHEMIA; MYOCARDIUM; NAD; OXYGEN; RABBITS;

EID: 84894104874     PISSN: 03636135     EISSN: 15221539     Source Type: Journal    
DOI: 10.1152/ajpheart.00696.2013     Document Type: Article

References (30)

1. Asfour H, Wengrowski AM, Jaimes III R, Swift LM, Kay MW. NADH fluorescence imaging of isolated biventricular working rabbit hearts. J Vis Exp. In print.
2. Ashruf JF, Coremans JM, Bruining HA, Ince C. Increase of cardiac work is associated with decrease of mitochondrial NADH. Am J Physiol Heart Circ Physiol 269: H856-H862, 1995.
3. Blinova K, Levine RL, Boja ES, Griffiths GL, Shi ZD, Ruddy B, Balaban RS. Mitochondrial NADH fluorescence is enhanced by complex I binding. Biochemistry 47: 9636-9645, 2008.
4. Bose S, French S, Evans FJ, Joubert F, Balaban RS. Metabolic network control of oxidative phosphorylation: multiple roles of inorganic phosphate. J Biol Chem 278: 39155-39165, 2003.
5. 2+ increases the mitochondrial NADH concentration during elevated work in intact cardiac muscle. Circ Res 80: 82-87, 1997.
6. 2+ during increased work in intact rat heart trabeculae. Biophys J 83: 587-604, 2002.
7. Chen V, Chen YH, Downing SE. An improved isolated working rabbit heart preparation using red cell enhanced perfusate. Yale J Biol Med 60: 209-219, 1987.
8. 2+ in the function and dysfunction of heart mitochondria. In: Calcium and the Heart, edited by Langer G. New York: Raven Press, 1990, p. 167-198.
9. Demmy TL, Magovern GJ, Kao RL. Isolated biventricular working rat heart preparation. Ann Thorac Surg 54: 915-920, 1992.
10. Farman GP, Tachampa K, Mateja R, Cazorla O, Lacampagne A, de Tombe PP. Blebbistatin: use as inhibitor of muscle contraction. Pflügers Arch 455: 995-1005, 2008.
11. Fedorov VV, Lozinsky IT, Sosunov EA, Anyukhovsky EP, Rosen MR, Balke CW, Efimov IR. Application of blebbistatin as an excitationcontraction uncoupler for electrophysiologic study of rat and rabbit hearts. Hear Rhythm 4: 619-626, 2007.
12. Gibbs C. The cytoplasmic phosphorylation potential. Its possible role in the control of myocardial respiration and cardiac contractility. J Mol Cell Cardiol 17: 727-731, 1985.
13. Gillis AM, Kulisz E, Mathison HJ. Cardiac electrophysiological variables in blood-perfused and buffer-perfused, isolated, working rabbit heart. Am J Physiol Heart Circ Physiol 271: H784-H789, 1996.
14. Heineman FW, Balaban RS. Effects of afterload and heart rate on NAD(P)H redox state in the isolated rabbit heart. Am J Physiol Heart Circ Physiol 264: H433-H440, 1993.
15. Ince C, Ashruf JF, Avontuur JA, Wieringa PA, Spaan JA, Bruining HA. Heterogeneity of the hypoxic state in rat heart is determined at capillary level. Am J Physiol Heart Circ Physiol 264: H294-H301, 1993.
16. Ingwall JS. Energy metabolism in heart failure and remodelling. Cardiovasc Res 81: 412-419, 2009.
17. Katz a L, Koretsky AP, Balaban RS. Respiratory control in the glucose perfused heart. A 31P NMR and NADH fluorescence study. FEBS Lett 221: 270-276, 1987.
18. Kay MW, Swift LM, Sangave A, Zderic V. High resolution contrast ultrasound and NADH fluorescence imaging of myocardial perfusion in excised rat hearts. Conf Proc IEEE Eng Med Biol Soc 2008: 969-972, 2008.
19. Kedem J, Breuer J, Acad BA, Sonn J. Coronary vasodilation produced by tachycardia under various basal flow conditions. Arch Int Physiol Biochim 89: 287-294, 1981.
20. Koretsky AP, Katz LA, Balaban RS. Determination of pyridine nucleotide fluorescence from the perfused heart using an internal standard. Am J Physiol Heart Circ Physiol 253: H856-H862, 1987.
21. Kour J, Ahmed JA, Aarif O, Kour J, Ahmed JA, Aarif O. Impact of heat stress on electrocardiographic changes in New Zealand white rabbits impact of heat stress on electrocardiographic changes in New Zealand white rabbits. 9: 242-252, 2013.
22. Langendorff O. [Investigations on the surviving mammalian heart.] Arch Gesante Physiol 61: 291-332, 1895.
23. Masuyama T, Uematsu M, Doi Y, Yamamoto K, Mano T, Naito J, Kondo H, Nagano R, Hori M, Kamada T. Abnormal coronary flow dynamics at rest and during tachycardia associated with impaired left ventricular relaxation in humans: implication for tachycardia-induced myocardial ischemia. J Am Coll Cardiol 24: 1625-1632, 1994.
24. McCormack JG, Halestrap AP, Denton RM. Role of calcium ions in regulation of mammalian intramitochondrial metabolism. Physiol Rev 70: 391-425, 1990.
25. Opie LH. Metabolism of the heart in health and disease. Am Heart J 76: 685-698, 1968.
26. Rothstein EC, Carroll S, Combs CA, Jobsis PD, Balaban RS. Skeletal muscle NAD(P)H two-photon fluorescence microscopy in vivo: topology and optical inner filters. Biophys J 88: 2165-2176, 2005.
27. +-ATPase in guinea-pig cardiac ventricular muscle. J Physiol 481. 3: 647-662, 1994.
28. Swift LM, Asfour H, Posnack NG, Arutunyan A, Kay MW, Sarvazyan N. Properties of blebbistatin for cardiac optical mapping and other imaging applications. Pflügers Arch Eur J Physiol 464: 503-512, 2012.
29. Tune JD. Matching coronary blood flow to myocardial oxygen consumption. J Appl Physiol 97: 404-415, 2004.
30. Wégria R, Frank CW, Wang H. The Effect of Atrial and Ventricular Tachycardia on Cardiac Output, Coronary Blood Flow and Mean Arterial Pressure. Circ Res 6: 624-632, 1958.