Non-specific binding in solid phase immunoassays for autoantibodies correlates with inflammation markers

Journal of Immunological Methods

Volumn 403, Issue 1-2, 2014, Pages 26-36

  Güven, Esin ^a   Duus, Karen ^a   Lydolph, Magnus Christian ^a   Jørgensen, Charlotte Sværke ^a   Laursen, Inga ^a   Houen, Gunnar ^a  

^a STATENS SERUM INSTITUT   (Denmark)

Author keywords

Autoantibodies; Elevated immunoglobulin concentration; ELISA; Inflammation; Non specific binding

Indexed keywords

AUTOANTIBODIES; ELEVATED IMMUNOGLOBULIN CONCENTRATION; ELISA; INFLAMMATION; NON-SPECIFIC BINDING;

ANTIBODY SPECIFICITY; AUTOANTIBODIES; AUTOANTIGENS; BINDING SITES, ANTIBODY; BIOLOGICAL MARKERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FALSE POSITIVE REACTIONS; HUMANS; IMMUNOGLOBULIN G; INFLAMMATION MEDIATORS; PREDICTIVE VALUE OF TESTS; PROTEIN DENATURATION; PROTEIN STABILITY; TEMPERATURE;

EID: 84893762788     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.11.014     Document Type: Article

References (30)

1. Andersen D.C., Koch C., Jensen C.H., Skjødt K., Brandt J., Teisner B. High prevalence of human anti-bovine IgG antibodies as the major cause of false positive reactions in two-site immunoassays based on monoclonal antibodies. J. Immunoass. Immunochem. 2004, 25:17.
2. Andersson J., Ekdahl K.N., Larsson R., Nilsson U.R., Nilsson B. C3 adsorbed to a polymer surface can form an initiating alternative pathway convertase. J. Immunol. 2002, 168:5786.
3. Butler J.E. Enzyme-linked immunosorbent assay. J. Immunoassay 2000, 21:165.
4. Cohen Tervaert J.W., Damoiseaux J. Antineutrophil cytoplasmic autoantibodies: how are they detected and what is their use for diagnosis, classification and follow-up?. Clin. Rev. Allergy Immunol. 2012, 43:211.
5. Cole L.A., Rinne K.M., Shahabi S., Omrani A. False-positive hCG assay results leading to unnecessary surgery and chemotherapy and needless occurrences of diabetes and coma. Clin. Chem. 1999, 45:313.
6. Cowchock S., Fort J., Munoz S., Norberg R., Maddrey W. False positive ELISA tests for anticardiolipin antibodies in sera from patients with repeated abortion, rheumatologic disorders and primary biliary cirrhosis: correlation with elevated polyclonal IgM and implications for patients with repeated abortion. Clin. Exp. Immunol. 1988, 73:289.
7. Despres N., Grant A.M. Antibody interference in thyroid assays: a potential for clinical misinformation. Clin. Chem. 1998, 44:440.
8. Dispenzieri A., Gertz M.A., Therneau T.M., Kyle R.A. Retrospective cohort study of 148 patients with polyclonal gammopathy. Mayo Clin. Proc. 2001, 76:476.
9. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971, 8:871.
10. Hernandez-Molina G., Leal-Alegre G., Michel-Peregrina M. The meaning of anti-Ro and anti-La antibodies in primary Sjogren's syndrome. Autoimmun. Rev. 2011, 10:123.
11. Hu B., Even C., Plagemann P.G. Immune complexes that bind to ELISA plates not coated with antigen in mice infected with lactate dehydrogenase-elevating virus: relationship to IgG2a- and IgG2b-specific polyclonal activation of B cells. Viral Immunol. 1992, 5:27.
12. Jethwa H.S., Nachman P.H., Falk R.J., Jennette J.C. False-positive myeloperoxidase binding activity due to DNA/anti-DNA antibody complexes: a source for analytical error in serologic evaluation of anti-neutrophil cytoplasmic autoantibodies. Clin. Exp. Immunol. 2000, 121:544.
13. Jørgensen C.S., Hansen K.B., Jacobsen S., Halberg P., Ullman S., Hansen D., Mikkelsen T.L., Weile B., Madsen M.H., Wiik A., Houen G. Absence of high-affinity calreticulin autoantibodies in patients with systemic rheumatic diseases and coeliac disease. Scand. J. Clin. Lab. Invest. 2005, 65:403.
14. Kaplan I.V., Levinson S.S. When is a heterophile antibody not a heterophile antibody? When it is an antibody against a specific immunogen. Clin. Chem. 1999, 45:616.
15. Kricka L.J. Human anti-animal antibody interferences in immunological assays. Clin. Chem. 1999, 45:942.
16. Levinson S.S. Antibody multispecificity in immunoassay interference. Clin. Biochem. 1992, 25:77.
17. Levinson S.S., Miller J.J. Towards a better understanding of heterophile (and the like) antibody interference with modern immunoassays. Clin. Chim. Acta 2002, 325:1.
18. Lewis J.G., Florkowski C.M., Elder P.A., Hunt P.J. Rheumatoid factor and false positive sex-hormone binding globulin. Clin. Chim. Acta 2003, 332:139.
19. McFarlane I.G., Smith H.M., Johnson P.J., Bray G.P., Vergani D., Williams R. Hepatitis C virus antibodies in chronic active hepatitis: pathogenetic factor or false-positive result?. Lancet 1990, 335:754.
20. Meroni P.L., Borghi M.O., Raschi E., Tedesco F. Pathogenesis of antiphospholipid syndrome: understanding the antibodies. Nat. Rev. Rheumatol. 2011, 7:330.
21. Moutsopoulos H.M., Zerva L.V. Anti-Ro (SSA)/La (SSB) antibodies and Sjogren's syndrome. Clin. Rheumatol. 1990, 9(Suppl. 1):123.
22. Nilsson U.R., Storm K.E., Elwing H., Nilsson B. Conformational epitopes of C3 reflecting its mode of binding to an artificial polymer surface. Mol. Immunol. 1993, 30:211.
23. Peterfi Z., Kocsis B. Comparison of blocking agents for and ELISA for LPS. J. Immunoassay 2000, 21:341.
24. Reen D.J. Enzyme-linked immunosorbent assay (ELISA). Methods Mol. Biol. 1994, 32:461.
25. Schultz D.R., Tozman E.C. Antineutrophil cytoplasmic antibodies: major autoantigens, pathophysiology, and disease associations. Semin. Arthritis Rheum. 1995, 25:143.
26. Shovman O., Gilburd B., Barzilai O., Langevitz P., Shoenfeld Y. Novel insights into associations of antibodies against cardiolipin and beta2-glycoprotein I with clinical features of antiphospholipid syndrome. Clin. Rev. Allergy Immunol. 2007, 32:145.
27. van Weemen B.K., Schuurs A.H. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971, 15:232.
28. Voller A., Bidwell D.E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull. World Health Organ. 1976, 53:55.
29. Willman J.H., Martins J.H., Jaskowski T.D., Hill H.R., Litwin C.M. Heterophile antibodies to bovine and caprine proteins causing false-positive human immunodeficiency virus type 1 and other enzyme-linked immunosorbent assay results. Clin. Diagn. Lab. Immunol. 1999, 6:615.
30. Xiao Y., Isaacs S.N. Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA) - not all BSAs are alike. J. Immunol. Methods 2012, 384:148.