Enhanced detection of Legionnaires' disease by PCR testing of induced sputum and throat swabs

European Respiratory Journal

Volumn 43, Issue 2, 2014, Pages 644-646

  Maze, Michael J ^a   Slow, Sandy ^b   Cumins, Anne Marie ^a   Boon, Kenjin ^a   Goulter, Patricia ^a   Podmore, Roslyn G ^c   Anderson, Trevor P ^c   Barratt, Kevin ^c   Young, Sheryl A ^c   Pithie, Alan D ^a   Epton, Michael J ^a,d   Werno, Anja M ^c   Chambers, Stephen T ^a,b   Murdoch, David R ^b,c  

^a CHRISTCHURCH HOSPITAL   (New Zealand)

^b UNIVERSITY OF OTAGO   (New Zealand)

^c CANTERBURY HEALTH LABORATORIES   (New Zealand)

^d Canterbury Resp Research Group   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; FEMALE; HUMANS; LEGIONELLA; LEGIONNAIRES' DISEASE; MALE; MIDDLE AGED; NEW ZEALAND; PHARYNX; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPUTUM; YOUNG ADULT;

EID: 84893457582     PISSN: 09031936     EISSN: 13993003     Source Type: Journal    
DOI: 10.1183/09031936.00191913     Document Type: Letter

References (10)

1. Diederen BM. Legionella spp. and Legionnaires' disease. J Infect 2008; 56: 1-12.
2. Murdoch DR. Diagnosis of Legionella infection. Clin Infect Dis 2003; 36: 64-69.
3. Murdoch DR, Podmore RG, Anderson TP, et al. Impact of routine systematic PCR testing on case finding for Legionnaires' disease: a pre-post comparison study. Clin Infect 2013; 57: 1275-1281.
4. Reischl U, Linde H-J, Lehn N, et al. Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-colour real-time PCR and melting curve analysis. J Clin Microbiol 2002; 40: 3814-3817.
5. Yang G, Benson R, Pelish T, et al. Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region. Clin Microbiol Infect 2010; 16: 255-261.
6. Diederen BM, Kluytmans AJW, Vandenbroucke-Grauls CM, et al. Utility of real-time PCR for diagnosis of Legionnaires' disease in routine clinical practice. J Clin Microbiol 2008; 46: 671-677.
7. Pizzichini E, Pizzichini MMM, Leigh R, et al. Safety of sputum induction. Eur Respir J 2002; 20: Suppl. 37, 9s-18s.
8. Diederen BM, Peeters MF. Are oropharyngeal swabs suitable as samples for Legionella-specific PCR testing? J Clin Microbiol 2007; 45: 3482-3483.
9. McDonough EA, Metzgar D, Hansen CJ, et al. A cluster of Legionella-associated pneumonia cases in a population of military recruits. J Clin Microbiol 2007; 45: 2075-2077.
10. Ramirez JA, Ahkee S, Tolentino A, et al. Diagnosis of Legionella pneumophila, Mycoplasma pneumoniae, or Chlamydia pneumoniae lower respiratory infection using the polymerase chain reaction on a single throat swab specimen. Diagn Microbiol Infect Dis 1996; 24: 7-14.