Cryoloading: Introducing large molecules into live synaptosomes

Frontiers in Cellular Neuroscience

Volumn 8, Issue JAN, 2014, Pages

  Nath, Arup R ^a   Chen, Robert H C ^a   Stanley, Elise F ^a  

^a UNIVERSITY HEALTH NETWORK   (Canada)

Author keywords

Intracellular; Peptide loading; Presynaptic; Recycling; Styryl dye; Synaptic vesicle; Synaptosome

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; BOTULINUM TOXIN A; CALCIUM CHANNEL; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; SNARE PROTEIN;

ANIMAL TISSUE; ARTICLE; BIOLOGICAL ACTIVITY; CALCIUM SIGNALING; CHICK; CONTROLLED STUDY; DEPOLARIZATION; ENDOCYTOSIS; EXOCYTOSIS; FLUORESCENCE MICROSCOPY; FREEZING; NONHUMAN; POSTSYNAPTIC MEMBRANE; SYNAPSE VESICLE; SYNAPTOSOME; TRANSMISSION ELECTRON MICROSCOPY;

EID: 84893009221     PISSN: 16625102     EISSN: None     Source Type: Journal    
DOI: 10.3389/fncel.2014.00004     Document Type: Article

References (26)

1. Adler, E. M., Augustine, G. J., Duffy, S. N., and Charlton, M. P. (1991). Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse. J. Neurosci. 11, 1496-1507.
2. Begley, J. G., Butterfield, D. A., Keller, J. N., Koppal, T., Drake, J., and Mattson, M. P. (1998). Cryopreservation of rat cortical synaptosomes and analysis of glucose and glutamate transporter activities and mitochondrial function. Brain Res. Brain Res. Protoc. 3, 76-82. doi: 10.1016/s1385-299x(98)00024-5
3. Bouvier, J. A., Charvin, N., Boudier, J. L., Fathallah, M., Tagaya, M., Takahashi, M., et al. (1996). Distribution of components of the SNARE complex in relation to transmitter release sites at the frog neuromuscular junction. Eur. J. Neurosci. 8, 545-552. doi: 10.1111/j.1460-9568.1996.tb01239.x
4. Chen, S., Zheng, X., Schulze, K. L., Morris, T., Bellen, H., and Stanley, E. F. (2002). Enhancement of presynaptic calcium current by cysteine string protein. J. Physiol. 538, 383-389. doi: 10.1113/jphysiol.2001.013397
5. Daniel, J. A., Malladi, C. S., Kettle, E., McCluskey, A., and Robinson, P. J. (2012). Analysis of synaptic vesicle endocytosis in synaptosomes by high-content screening. Nat. Protoc. 7, 1439-1455. doi: 10.1038/nprot.2012.070
6. Forsythe, I. D. (1994). Direct patch recording from identified presynaptic terminals mediating glutamatergic EPSCs in the rat CNS, in vitro. J. Physiol. 479, 381-387.
7. Gleitz, J., Beile, A., Wilffert, B., and Tegtmeier, F. (1993). Cryopreservation of freshly isolated synaptosomes prepared from the cerebral cortex of rats. J. Neurosci. Methods 47, 191-197. doi: 10.1016/0165-0270(93)90081-2
8. Juhaszova, M., Church, P., Blaustein, M. P., and Stanley, E. F. (2000). Location of calcium transporters at presynaptic terminals. Eur. J. Neurosci. 12, 839-846. doi: 10.1046/j.1460-9568.2000.00974.x
9. Khanna, R., Li, Q., Bewersdorf, J., and Stanley, E. F. (2007). The presynaptic CaV2.2 channel-transmitter release site core complex. Eur. J. Neurosci. 26, 547-559. doi: 10.1111/j.1460-9568.2007.05680.x
10. Li, Q., Lau, A., Morris, T. J., Guo, L., Fordyce, C. B., and Stanley, E. F. (2004). A syntaxin 1, Galpha(o) and N-type calcium channel complex at a presynaptic nerve terminal: analysis by quantitative immunocolocalization. J. Neurosci. 24, 4070-4081. doi: 10.1523/jneurosci.0346-04.2004
11. Llinas, R. R., Steinberg, I. Z., and Walton, K. (1981). Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse. Biophys. J. 33, 323-351. doi: 10.1016/s0006-3495(81)84899-0
12. Low, P., Norlin, T., Risinger, C., Larhammar, D., Pieribone, V. A., Shupliakov, O., et al. (1999). Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function. Eur. J. Cell Biol. 78, 787-793. doi: 10.1016/s0171-9335(99)80029-6
13. Martin, A. R., and Ringham, G. L. (1975). Synaptic transfer at a vertebrate central nervous system synapse. J. Physiol. 251, 409-426.
14. Miledi, R. (1969). Transmitter release induced by injection of calcium ions into nerve terminals. Proc. R. Soc. Lond. B Biol. Sci. 183, 421-425. doi: 10.1098/rspb.1973.0026
15. Nichols, R. A., Wu, W. C., Haycock, J. W., and Greengard, P. (1989). Introduction of impermeant molecules into synaptosomes using freeze/thaw permeabilization. J. Neurochem. 52, 521-529. doi: 10.1111/j.1471-4159.1989.tb09151.x
16. Picconi, B., Piccoli, G., and Calabresi, P. (2012). Synaptic dysfunction in Parkinson's disease. Adv. Exp. Med. Biol. 970, 553-572. doi: 10.1007/978-3-7091-0932-8_24
17. Schiavo, G., Rossetto, O., Tonello, F., and Montecucco, C. (1995). Intracellular targets and metalloprotease activity of tetanus and botulism neurotoxins. Curr. Top. Microbiol. Immunol. 195, 257-274. doi: 10.1007/978-3-642-85173-5_12
18. Stanley, E. F. (1989). Calcium currents in a vertebrate presynaptic nerve terminal: the chick ciliary ganglion calyx. Brain Res. 505, 341-345. doi: 10.1016/0006-8993(89)91465-0
19. Stanley, E. F. (1993). Single calcium channels and acetylcholine release at a presynaptic nerve terminal. Neuron 11, 1007-1011. doi: 10.1016/0896-6273(93)90214-c
20. Stanley, E. F., and Goping, G. (1991). Characterization of a calcium current in a vertebrate cholinergic presynaptic nerve terminal. J. Neurosci. 11, 985-993. doi: 10.1016/0896-6273(91)90371-6
21. Tamura, Y., Ozkan, E. D., Bole, D. G., and Ueda, T. (2001). IPF, a vesicular uptake inhibitory protein factor, can reduce the Ca(2+).-dependent, evoked release of glutamate, GABA and serotonin. J. Neurochem. 76, 1153-1164. doi: 10.1046/j.1471-4159.2001.00120.x
22. Tsien, R. Y. (1981). A non-disruptive technique for loading calcium buffers and indicators into cells. Nature 290, 527-528. doi: 10.1038/290527a0
23. Watanabe, H., Yamashita, T., Saitoh, N., Kiyonaka, S., Iwamatsu, A., Campbell, K. P., et al. (2010). Involvement of Ca2+ channel synprint site in synaptic vesicle endocytosis. J. Neurosci. 30, 655-660. doi: 10.1523/jneurosci.3214-09.2010
24. Whittaker, V. P. (1993). Thirty years of synaptosome research. J. Neurocytol. 22, 735-742. doi: 10.1007/bf01181319
25. Wong, F. K., Li, Q., and Stanley, E. F. (2013). Synaptic vesicle capture by CaV2.2 calcium channels. Front. Cell. Neurosci. 7:101. doi: 10.3389/fncel.2013.00101
26. Yao, P. J., Zhu, M., Pyun, E. I., Brooks, A. I., Therianos, S., Meyers, V. E., et al. (2003). Defects in expression of genes related to synaptic vesicle trafficking in frontal cortex of Alzheimer's disease. Neurobiol. Dis. 12, 97-109. doi: 10.1016/s0969-9961(02)00009-8