Comparative analysis of Edwardsiella isolates from fish in the eastern United States identifies two distinct genetic taxa amongst organisms phenotypically classified as E. tarda

Veterinary Microbiology

Volumn 165, Issue 3-4, 2013, Pages 358-372

  Griffin, Matt J ^a   Quiniou, Sylvie M ^b   Cody, Theresa ^c   Tabuchi, Maki ^c   Ware, Cynthia ^a   Cipriano, Rocco C ^d   Mauel, Michael J ^e   Soto, Esteban ^f  

^a MISSISSIPPI STATE UNIVERSITY   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c FISH AND WILDLIFE RESEARCH INSTITUTE   (United States)

^d LEETOWN SCIENCE CENTER   (United States)

^e MISSISSIPPI STATE UNIVERSITY   (United States)

^f ROSS UNIVERSITY SCHOOL OF MEDICINE   (Dominica)

Author keywords

Blue catfish; Channel catfish; Edwardsiella tarda; Hybrid striped bass; Multilocus sequencing; PCR; Rep PCR; Tilapia

Indexed keywords

AMIKACIN; AMPICILLIN; AZTREONAM; BACTERIAL DNA; CEFAZOLIN; CEFEPIME; CEFOTETAN; CEFOXITIN; CEFTAZIDIME; CEFTRIAXONE; CEFUROXIME; CHAPERONIN 60; COTRIMOXAZOLE; CYTOCHROME C OXIDASE; DNA 16S; DNA DIRECTED DNA POLYMERASE ALPHA; DNA TOPOISOMERASE (ATP HYDROLYSING) A; DNA TOPOISOMERASE (ATP HYDROLYSING) B; FATTY ACID; FLORFENICOL; GATIFLOXACIN; GENOMIC DNA; GLUCOSE 6 PHOSPHATE ISOMERASE; IMIPENEM; MEROPENEM; PHOSPHATE TRANSPORTER; PHOSPHOGLUCOMUTASE; RIBOSOME DNA; SULTAMICILLIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENETICS; BACTERIAL GROWTH; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CARBON SOURCE; CARBON UTILIZATION; CATFISH; CHANNEL CATFISH; CONTROLLED STUDY; DNA BASE COMPOSITION; DNA EXTRACTION; DNA SEQUENCE; DNA TEMPLATE; EDWARDSIELLA ICTALURI; EDWARDSIELLA TARDA; ESCHERICHIA COLI; FLUOROMETRY; GENE CLUSTER; GENETIC IDENTIFICATION; GENETIC VARIABILITY; GENOTYPE; LIPID COMPOSITION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; STRIPED BASS; TILAPIA; UNITED STATES;

EDWARDSIELLA (ENTEROBACTERIA); EDWARDSIELLA ICTALURI; EDWARDSIELLA TARDA; ENTEROBACTERIACEAE; ICTALURUS FURCATUS; ICTALURUS PUNCTATUS; MORONE; NEGIBACTERIA; TILAPIA;

ANIMALS; ANTI-INFECTIVE AGENTS; BASE COMPOSITION; EDWARDSIELLA TARDA; ENTEROBACTERIACEAE INFECTIONS; FISH DISEASES; FISHES; GENES, BACTERIAL; GENETIC VARIATION; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; UNITED STATES;

EID: 84892999937     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2013.03.027     Document Type: Article

References (36)

1. Acharya M., Maiti N.K., Mohanty S., Mishra P., Samanta M. Genotyping of Edwardsiella tarda isolated from freshwater culture system. Comp. Immunol. Microbiol. Infect. Dis. 2007, 30:33-40.
2. Castro N., Toranzo A.E., Barja J.L., Núñez S., Magariños B. Characterization of Edwardsiella tarda strains isolated from turbot, Psetta maxima (L.). J. Fish Dis. 2006, 29:541-547.
3. Castro N., Toranzo A.E., Núñez S., Osorio C.R., Magariños B. Evaluation of four polymerase chain reaction primer pairs for the detection of Edwardsiella tarda in turbot. Dis. Aquat. Organ. 2010, 18:55-61.
4. Castro N., Toranzo A.E., Bastardo A., Barja J.L., Magariños B. Intraspecific genetic variability of Edwardsiella tarda strains from cultured turbot. Dis. Aquat. Organ. 2011, 95:253-258.
5. Chen J.D., Lai S.Y. PCR for direct detection of Edwardsiella tarda from infected fish and environmental water by application of the hemolysin gene. Zool. Stud. 1998, 37:169-176.
6. Clarridge J.E. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev. 2004, 17:840-862.
7. Cooper, J.E., Feil, E.J., 2004. Multilocus sequence typing-what is resolvedα Trends Microbiol. 12, 373-377.
8. Ewing W.H., Mcwhorter A.C., Escobar M.R., Lubin A.H. Edwardsiella, a new genus of Enterobacteriaceae based on a new species, E. tarda. Int. J. Syst. Evol. Microbiol. 1965, 15:33-38.
9. Felsentein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985, 39:783-791.
10. Fox G.E., Wisotzkey J.D., P.Jurtshuk P.J.R. How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int. J. Syst. Bacteriol. 1992, 42:166-170.
11. Gonzalez J.M., Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 2002, 4:770-773.
12. Griffin M.J., Mauel M.J., Greenway T.E., Khoo L.H., Wise D.J. A real-time polymerase chain reaction assay for quantification of Edwardsiella ictaluri in catfish pond water and genetic homogeneity of diagnostic case isolates from Mississippi. J. Aquat. Anim. Health. 2011, 23:178-188.
13. Hawke J.P., Khoo L.H. Infectious diseases. Biology and Culture of the Channel Catfish 2004, 387-443. Elsevier B.V., Amsterdam, The Netherlands. C.S. Tucker, J.A. Hargreaves (Eds.).
14. Janda J.M., Abbott S.L. Bacterial identification for publication: when is enough enoughα. J. Clin. Microbiol. 2002, 40:1887-1891.
15. Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 1980, 16:111-120.
16. Lan J., Zhang X.H., Wang Y., Chen J., Han Y. Isolation of an unusual strain of Edwardsiella tarda from turbot and establish a PCR detection technique with the gyrB gene. J. Appl. Microbiol. 2008, 105:644-651.
17. Lennette E.H., Ballows A., Hauser W.J., Shadomy H.J. Manual of Clinical Microbiology 1985, American Society for Microbiology, Washington, DC, 1149 pp. 4th edition.
18. MacFaddin J.F. Biochemical Tests for Identification of Medical Bacteria 1981, Williams and Wilkins, Baltimore/London, 527 pp. 2nd edition.
19. Maiti N.K., Mandal A., Mohanty S., Samanta M. Comparative analysis of genome of Edwardsiella tarda by BOX-PCR and PCR-ribotyping. Aquaculture 2008, 280:60-63.
20. Maiti N.K., Mandal A., Mohanty S., Mandal R.N. Phenotypic and genetic characterization of Edwardsiella tarda isolated from pond sediments. Comp. Immunol. Microb. 2009, 32:1-8.
21. Meyer F.P., Bullock G.L. Edwardsiella tarda, a new pathogen of channel catfish (Ictalurus punctatus). Appl. Microbiol. 1973, 25:155-156.
22. Mohanty B.R., Sahoo P.K. Edwardsiellosis in fish: a brief review. J. Biosci. 2007, 32:1331-1344.
23. Panangala V.S., Shoemaker C.A., McNulty S.T., Arias C.R., Klesius P.H. Intra- and interspecific phenotypic characteristics of fish-pathogenic Edwardsiella ictaluri and E. tarda. Aquacult. Res. 2006, 37:49-60.
24. T contains multiple 16S rRNA genes with heterogenous intervening sequences. Microbiology 1996, 142:2087-2095.
25. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987, 4:406-425.
26. Sakai T., Iida T., Osatomi K., Kanai K. Detection of Type 1 fimbrial genes in fish pathogenic and non-pathogenic Edwardsiella tarda strains by PCR. Fish Pathol. 2007, 42:115-117.
27. Sakai T., Yuasa K., Sano M., Iida T. Identification of Edwardsiella ictaluri and E. tarda by species-specific polymerase chain reaction targeted to the upstream region of the fimbrial gene. J. Aquat. Anim. Health. 2009, 21:124-132.
28. Sasser, M., 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc., Newark, Delaware.
29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 2011, 28:2731-2739.
30. van Soest J., Stockhammer O.W., Ordas A., Bloemberg G.V., Spaink H.P., Meijer A.H. Comparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda. BMC Immunobiol. 2011, 12:58. 10.1186/1471-2172-12-58.
31. Versalovic J., Koeuth T., Lupski J.R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991, 19:6823-6831.
32. Versalovic J., Schneider M., de Bruijn F.J., Lupski J.R. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Method Mol. Cell. Biol. 1994, 5:25-40.
33. Wang Q., Yang M., Xiao J., Wu H., Wang X., Lv Y., Xu L., Zheng H., Wang S., Zhao G., Liu Q., Zhang Y. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS ONE 2009, 4:e7646. 10.1371/journal.pone.0007646.
34. Wang Y.M., Wang Q.Y., Xiao J.F., Liu Q., Wu H.Z., Zhang Y.X. Genetic relationships of Edwardsiella strains isolated in China aquaculture revealed by rep-PCR genomic fingerprinting and investigation of Edwardsiella virulence genes. J. Appl. Microbiol. 2011, 111:1337-1348.
35. Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Banes M.M., Lawrence M.L. Genome sequence of Edwardsiella ictaluri 93-146, a strain associated with a natural channel catfish outbreak of enteric septicemia of catfish. J. Bacteriol. 2012, 194:740-741.
36. Yang M., Lv Y., Xiao J., Wu H., Zheng H., Liu Q., Zhang Y., Wang Q. Edwardsiella comparative phylogenomics reveal the new intra/interspecies taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLoS ONE 2012, 7(5):e36897.