Directing rnase p-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells

Methods in Molecular Biology

Volumn 1103, Issue , 2014, Pages 45-56

  Jiang, Xiaohong ^a   Sunkara, Naresh ^a   Lu, Sangwei ^a   Liu, Fenyong ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Engineered external guide sequence; Gene targeting; Human cytomegalovirus (HCMV); Human RNase P; In vitro evaluation

Indexed keywords

MESSENGER RNA; RIBONUCLEASE P; RNA; RNA POLYMERASE; PROTEINASE; TRANSFER RNA;

ARTICLE; CELL CULTURE; CELL GROWTH; DISSOCIATION CONSTANT; DNA SEQUENCE; EXTERNAL GUIDE SEQUENCE; GENE EXPRESSION; GENE SEQUENCE; HUMAN; HUMAN CYTOMEGALOVIRUS; IN VITRO SELECTION; IN VITRO STUDY; MICHAELIS CONSTANT; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA CLEAVAGE; STABLE EXPRESSION; VIRUS CULTURE; VIRUS EXPRESSION; VIRUS GENE; ANIMAL CELL; ANTICODON; CODING; GENETIC ENGINEERING; HUMAN CELL; RNA INTERFERENCE; VIROGENESIS; VIRUS GENOME; WESTERN BLOTTING; CONFORMATION; CYTOMEGALOVIRUS; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HELA CELL LINE; MOLECULAR BIOLOGY; PATHOGENICITY; PROCEDURES; VIRUS REPLICATION;

CYTOMEGALOVIRUS; GENE EXPRESSION REGULATION, VIRAL; HELA CELLS; HUMANS; MOLECULAR BIOLOGY; NUCLEIC ACID CONFORMATION; RIBONUCLEASE P; RNA, MESSENGER; VIRUS REPLICATION;

MLCS; MLOWN;

EID: 84892854355     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-730-3_4     Document Type: Article

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