Utilizing mAb adsorption isotherms to direct process development of a strong cation-exchange chromatography step

ACS Symposium Series

Volumn 1125, Issue , 2013, Pages 51-65

  Gillespie, Christopher ^a  

^a EMD Millipore   (United States)

Author keywords

Adsorption isotherms; Dynamic binding capacity; Langmuir isotherm; Linear gradient elution; MAb purification; Monoclonal antibodies; Static binding capacity

Indexed keywords

ADSORPTION ISOTHERMS; LOADING; MONOCLONAL ANTIBODIES; PH; PURIFICATION; SURFACE PLASMON RESONANCE;

BINDING CAPACITIES; DYNAMIC BINDING CAPACITY; LANGMUIR ISOTHERM; LINEAR GRADIENT ELUTION; MAB PURIFICATION;

CHROMATOGRAPHY;

EID: 84892744650     PISSN: 00976156     EISSN: 19475918     Source Type: Book Series    
DOI: 10.1021/bk-2013-1125.ch004     Document Type: Article

References (17)

1. For a review see: Oldham, R. K. Monoclonal antibodies in cancer therapy: 25 years of progress. J. Clinical Oncol. 2008, 26, 1774-1777.
2. Textbook reference: Rang, H. P.; Dale, M. M.; Ritter, J. M. Phamacology, 5th ed.; Churchill Livingstone: New York, 2003; p 241.
3. Waldmann, T. A. Monoclonal antibodies in diagnosis and therapy. Science 1991, 21, 1657-1662.
4. Bruno, V.; Battaglia, G.; Nicoletti, F. The advent of monoclonal antibodies in the treament of chronic autoimmune diseases. Neurol. Sci. 2011, 31, S283-S288.
5. Kieseir, B. C.; Lehmann, H. C.; zu Horste, G. M. Autoimmune diseases of the peripheral nervous system. Autoimmun. Rev. 2012, 11, 191-5.
6. Pescovitz, M. D. Rituximab, an anti-CD20 monoclonal antibody: history and mechanism of action. Am. J. Transplant. 2006, 6, 859-866.
7. Breedveld, F. C. Therapeutic monoclonal antibodies. Lancet, 2001, 355, 735-740.
8. Farid, S. S. Process economics of industrial monoclonal antibody manufacture. J. Chromatogr., B 2007, 848, 8-18.
9. Low, D.; O'Leary, R.; Pujar, N. S. Future of antibody purification. J. Chromatogr., B 2007, 848, 48-63.
10. Hacker, D. L.; Nallet, S.; Wurm, F. M. Recombinant protein production yields from mammalian cells: past, present, and future. Biopharm. Int. Suppl. June 2008, 21.
11. Terman, D. S.; Bertram, J. H. Antitumor effects of immobilized protein A and staphylococcal products: linkage between toxicity and efficacy, and identification of potential tumoricidal reagents. Eur. J. Cancer Clin. Oncol. 1985, 21, 1115-1122.
12. For additional information see: (b) Pabst, T. M.; Suda, E. J.; Thomas, K. E.; Mensah, P.; Ramasubramanyan, N.; Gustafson, M. E.; Hunter, A. K. Binding and elution behavior of proteins on strong cation exchangers. J. Chromatogr., A 2009, 1216, 7950-7956.
13. Ishihara, T.; Yamamoto, S. Optimization of monoclonal antibody purification by ion-exchange chromatography: Applications of simple methods with linear gradient elution experimental data. J. Chromatogr., A 2005, 1069, 99-106.
14. Ishihara, T.; Kadoya, T.; Endo, N.; Yamamoto, S. Optimization of elution salt concentration in stepwise elution of protein chromatography using linear gradient elution data: Reducing residual protein A by cation-exchange chromatography in monoclonal antibody purification. J. Chromatogr., A 2006, 1114, 97-101.
15. Urmann, M.; Graalfs, H.; Joehnck, M.; Jacob, L. R.; Frech, C. Cationexchange chromatography of monoclonal antibodies: characterization of a novel stationary phase designed for production-scale purification. MAbs 2010, 4, 395-404.
16. Cygnus Technologies. CHO Host Cell Proteins 3rd Generation, Cat # : F550, http://www.cygnustechnologies.com//images/file/Product-Inserts/f550-0-pi.pdf.
17. Xu, X.; Lenhoff, A. M. A predictive approach to correlating protein adsorption isotherms on ion-exchange media. J. Chem. Phys. B 2008, 112, 1028-1040.