Influence of parasite density and sample storage time on the reliability of entamoeba histolytica-specific PCR from formalin-fixed and paraffin-embedded tissues

Diagnostic Molecular Pathology

Volumn 22, Issue 4, 2013, Pages 236-244

  Frickmann, Hagen ^a,b   Tenner Racz, Klara ^c   Eggert, Petra ^c   Schwarz, Norbert G ^c   Poppert, Sven ^c   Tannich, Egbert ^c   Hagen, Ralf M ^a  

^a Bundeswehrkrankenhaus Hamburg   (Germany)

^b BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^c ROSTOCK UNIVERSITY MEDICAL CENTER   (Germany)

Author keywords

Diagnostic PCR; Entamoeba histolytica; Formalin fixed; Microscopy; Paraffin embedded tissue; PAS staining

Indexed keywords

DNA; FORMALDEHYDE; PARAFFIN; RNA 18S;

AGE; AMEBIASIS; ARTICLE; CONTROLLED STUDY; DNA DEGRADATION; EMBEDDING; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; FALSE NEGATIVE RESULT; FLUORESCENCE IN SITU HYBRIDIZATION; GENE TARGETING; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MICROBIOLOGICAL PARAMETERS; MICROORGANISM DETECTION; MICROSCOPY; PARASITE DENSITY; PREDICTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SHELF LIFE; TIME; TISSUE SECTION;

ENTAMOEBA HISTOLYTICA; ENTAMOEBIASIS; FALSE NEGATIVE REACTIONS; FORMALDEHYDE; HUMANS; MICROSCOPY; PARAFFIN; PARASITE LOAD; PARASITOLOGY; PATHOLOGY, MOLECULAR; REAL-TIME POLYMERASE CHAIN REACTION; SPECIMEN HANDLING; TIME FACTORS; TISSUE FIXATION;

EID: 84892719705     PISSN: 10529551     EISSN: 15334066     Source Type: Journal    
DOI: 10.1097/PDM.0b013e3182936936     Document Type: Article

References (40)

1. Ximé nez C, Morá n P, Rojas L, et al. Novelties on amoebiasis: a neglected tropical disease. J Glob Infect Dis. 2011;3:166-174.
2. Lotter H, TFGH Jackson, Tannich E. Evaluation of three serological tests for the detection of antiamebic antibodies applied to sera of patients from an area endemic for amebiasis. Trop Med Parasitol. 1995;71:401-407.
3. Blessmann J, Buss H, Nu PA, et al. Real-Time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J Clin Microbiol. 2002;40:4413-4417.
4. Tanyuksel M, Petri WA Jr. Laboratory diagnosis of amebiasis. Clin Microbiol Rev. 2003;16:713-729.
5. Lebbad M, Svä rd SG. PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy. Scand J Infect Dis. 2005;37:680-685.
6. Blessmann J, Binh HD, Hung DM, et al. Treatment of amoebic liver abscess with metronidazole alone or in combination with ultrasound-guided needle aspiration: a comparative, prospective and randomized study. Trop Med Int Health. 2003;8:1030-1034.
7. Gilman R, Islam M, Paschi S, et al. Comparison of conventional and immunofluorescent techniques for the detection of Entamoeba histolytica in rectal biopsies. Gastroenterology. 1980;78:435-439.
8. Marcus VA, Ward BJ, Jutras P. Intestinal amebiasis: a diagnosis not to be missed. Pathol Res Pract. 2001;197:271-274.
9. Kebede A, Verweij JJ, Petros B, et al. Short communication: Misleading microscopy in amoebiasis. Trop Med Int Health. 2004; 9:651-652.
10. Richter J, Heintges T, Thomassen D, et al. An unsuspected cause of chronic colitis. Gut. 2006;55:832-841.
11. Koo JS, Choi WS, Park DW. Fulminant amebic colitis mimicking pseudomembranous colitis. Gastrointest Endosc. 2010;71:400-401.
12. Verweij JJ, Blangé RA, Templeton K, et al. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-Time PCR. J Clin Microbiol. 2004;42:1220-1223.
13. Roy S, Kabir M, Mondal D, et al. Real-Time-PCR assay for diagnosis of Entamoeba histolytica infection. J Clin Microbiol. 2005; 43:2168-2172.
14. Haque R, Roy S, Siddique A, et al. Multiplex real-Time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. Am J Trop Med Hyg. 2007;76:713-717.
15. Bruijnesteijn van Coppenraet LE, Wallinga JA, Ruijs GJ, et al. Parasitological diagnosis combining an internally controlled real-Time PCR assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy. Clin Microbiol Infect. 2009;15:869-874.
16. Hamzah Z, Petmitr S, Mungthin M, et al. Development of multiplex real-Time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens. Am J Trop Med Hyg. 2010;83:909-913.
17. Liang SY, Hsia KT, Chan YH, et al. Evaluation of a new single-Tube multiprobe real-Time PCR for diagnosis of Entamoeba histolytica and Entamoeba dispar. J Parasitol. 2010;96:793-797.
18. Stark D, Al-Qassab SE, Barratt JL, et al. Evaluation of multiplex tandem real-Time PCR for detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in clinical stool samples. J Clin Microbiol. 2011;49: 257-262.
19. Taniuchi M, Verweij JJ, Noor Z, et al. High throughput multiplex PCR and probe-based detection with Luminex beads for seven intestinal parasites. Am J Trop Med Hyg. 2011;84: 332-337.
20. Hagen RM, Gauthier YP, Sprague LD, et al. Strategies for PCR based detection of Burkholderia pseudomallei DNA in paraffin wax embedded tissues. Mol Pathol. 2002;55:398-400.
21. Volpini AC, Marques MJ, Lopes dos Santos S, et al. Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years. Clin Microbiol Infect. 2006;12: 815-818.
22. Costa CAX, Brito KNO, Gomes MA, et al. Histopathological and immunohistochemical study of the hepatic lesions experimentally induced by Entamoeba dispar. Eur J Histochem. 2010;54: 170-174.
23. Costa CA, Fonseca TH, Oliveira FM, et al. Influence of inflammation on parasitism and area of experimental amoebic liver abscess: an immunohistochemical and morphometric study. Parasite Vectors. 2011;4:27.
24. Dolabella SS, Serrano-Luna J, Navarro-Garcí a F, et al. Amoebic liver abscess production by Entamoeba dispar. Ann Hepatol. 2012; 11:107-117.
25. Shibayama M, Dolabella SS, Serrano-Luna J, et al. Amoebic liver abscess production by Entamoeba dispar. Ann Hepatol. 2012;11: 107-117.
26. Shibayama M, Dolabella SS, Silva EF, et al. A Brazilian species of Entamoeba dispar (ADO) produces amoebic liver abscess in hamsters. Ann Hepatol. 2007;6:117-118.
27. ten Hove RJ, van Esbroeck M, Vervoort T, et al. Molecular diagnostics of intestinal parasites in returning travellers. Eur J Clin Microbiol Infect Dis. 2009;28:1045-1053.
28. Kebede A, Verweij JJ, Endeshaw T, et al. The use of real-Time PCR to identify Entamoeba histolytica and E. dispar infections in prisoners and primary-school children in Ethiopia. Ann Trop Med Parasitol. 2004;98:43-48.
29. Bhattacharya S, Som I, Bhattacharya A. The ribosomal DNA plasmids of Entamoeba. Parasitol Today. 1994;14:181-185.
30. Silberman JD, Clark CG, Diamond LS, et al. Phylogeny of the genera Entamoeba and Endolimax as deduced from small-subunit ribosomal RNA sequences. Mol Biol Evol. 1999;16: 1740-1751.
31. Niesters HGM. Quantitation of viral load using real-Time amplification techniques. Methods. 2001;25:419-429.
32. Khot PD, Ko DL, Hackman R, et al. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. BMC Infect Dis. 2008;8:73.
33. Hagen RM, Frickmann H, Elschner M, et al. Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples. Int J Med Microbiol. 2011;301: 585-590.
34. Frickmann H, Alnamar Y, Essig A, et al. Rapid identification of Leishmania spp. in formalin-fixed, paraffin-embedded tissue samples by fluorescence in situ hybridization. Trop Med Int Health. 1995; 17:1117-1126.
35. Amann RI, Ludwig W, Schleifer KH. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev. 1995;59:143-169.
36. Petri WA Jr. Therapy of intestinal protozoa. Trends Parasitol. 2003;19:523-526.
37. Knobloch J, Mannweiler E. Development and persistence of antibodies to Entamoeba histolytica in patients with amebic liver abscess. Am J Trop Med Hyg. 1983;32:727-732.
38. Wynants H, Van den Ende J, Randria J, et al. Diagnosis of amoebic infection of the liver: report of 36 cases. Ann Soc Belg Med Trop. 1995;75:297-303.
39. Okhuysen PC. Traveler's diarrhea due to intestinal protozoa. Clin Infect Dis. 2001;33:110-114.
40. Rickerts V, Khot PD, Myerson D, et al. Comparison of quantitative real time PCR with sequencing and ribosomal RNA-FISH for the identification of fungi in formalin fixed, paraffin-embedded tissue specimens. BMC Infect Dis. 2011;11:202.