Transcriptional analysis of hnRNPA0, A1, A2, B1, and A3 in lung cancer cell lines in response to acidosis, hypoxia, and serum deprivation conditions

Experimental Lung Research

Volumn 40, Issue 1, 2014, Pages 12-21

  Romero Garcia, Susana ^a   Prado Garcia, Heriberto ^a   Lopez Gonzalez, Jose Sullivan ^a  

^a INSTITUTO NACIONAL DE ENFERMEDADES RESPIRATORIAS   (Mexico)

Author keywords

Alternative splicing; QRT PCR; Ribonucleoprotein; Tumor microenvironment

Indexed keywords

HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A2; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A3; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN B1; UNCLASSIFIED DRUG;

ACIDOSIS; ALTERNATIVE RNA SPLICING; ARTICLE; CANCER CELL; CELL SURVIVAL; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYPOXIA; LUNG CANCER; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SERUM; STRESS; TUMOR MICROENVIRONMENT;

ACIDOSIS; ANOXIA; CELL LINE, TUMOR; DOWN-REGULATION; HUMANS; LUNG; LUNG NEOPLASMS; RIBONUCLEOPROTEINS; TRANSCRIPTION, GENETIC; UP-REGULATION;

EID: 84892693065     PISSN: 01902148     EISSN: 15210499     Source Type: Journal    
DOI: 10.3109/01902148.2013.856049     Document Type: Article

References (35)

1. Han SP, Tang YH, Smith R: Functional diversity of the hnRNPs: past, present and perspectives. Biochem J. 2010; 430(3):379-392.
2. Ma AS, Moran-Jones K, Shan J, Munro TP, Snee MJ, Hoek KS, Smith R: Heterogeneous nuclear ribonucleoprotein A3, a novel RNA trafficking response element-binding protein. J Biol Chem. 2002;277(20):18010-18020.
3. Myer VE, Steitz JA: Isolation and characterization of a novel, low abundance hnRNP protein: A0. RNA. 1995;1(2):171-182.
4. Kamma H, Horiguchi H, Wan L, Matsui M, Fujiwara M, Fujimoto M, Yazawa T, Dreyfuss G: Molecular characterization of the hnRNP A2/B1 proteins: tissue-specific expression and novel isoforms. Exp Cell Res. 1999;246(2):399-411.
5. Han SP, Kassahn KS, Skarshewski A, Ragan MA, Rothnagel JA, Smith R: Functional implications of the emergence of alternative splicing in hnRNP A/B transcripts. RNA. 2010;16(9):1760-1768.
6. He Y, Brown MA, Rothnagel JA, Saunders NA, Smith R: Roles of heterogeneous nuclear ribonucleoproteins A and B in cell proliferation. J Cell Sci. 2005;118(Pt 14):3173-3183.
7. He Y, Smith R: Nuclear functions of heterogeneous nuclear ribonucleoproteins A/B. Cell Mol Life Sci. 2009;66(7): 1239-1256.
8. McGlincy NJ, Tan LY, Paul N, Zavolan M, Lilley KS, Smith CW: Expression proteomics of UPF1 knockdown in HeLa cells reveals autoregulation of hnRNP A2/B1 mediated by alternative splicing resulting in nonsense-mediated mRNA decay. BMC Genom. 2010;11:565.
9. David CJ, Manley JL: Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged. Genes Dev. 2010;24(21):2343-2364.
10. Choi HS, Lee HM, Jang YJ, Kim CH, Ryu CJ: Heterogeneous nuclear ribonucleoprotein A2/B1 regulates the selfrenewal and pluripotency of human embryonic stem cells via the control of the G1/S transition. Stem Cells. 2013. doi: 10.1002/stem.1366.
11. Zhu D, Xu G, Ghandhi S, Hubbard K: Modulation of the expression of p16INK4a and p14ARF by hnRNP A1 and A2 RNA binding proteins: implications for cellular senescence. J Cell Physiol. 2002;193(1):19-25.
12. Oh HK, Lee E, Jang HN, Lee J, Moon H, Sheng Z, Jun Y, Loh TJ, Cho S, Zhou J, Green MR, Zheng X, Shen H: hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene. Apoptosis. 2013;18(7):825-835.
13. Choi YH, Lim JK, Jeong MW, Kim KT: HnRNP A1 phosphorylated by VRK1 stimulates telomerase and its binding to telomeric DNA sequence. Nucleic Acids Res. 2012;40(17):8499-8518.
14. Zhou ZJ, Dai Z, Zhou SL, Fu XT, Zhao YM, Shi YH, Zhou J, Fan J: Overexpression of HnRNP A1 promotes tumor invasion through regulating CD44v6 and indicates poor prognosis for hepatocellular carcinoma. Int J Cancer. 2012;132(5):1080-1089.
15. Ma YL, Peng JY, Zhang P, Huang L, Liu WJ, Shen TY, Chen HQ, Zhou YK, ZhangM, Chu ZX,Qin HL: Heterogeneous nuclear ribonucleoprotein A1 is identified as a potential biomarker for colorectal cancer based on differential proteomics technology. J Proteome Res. 2009;8(10):4525-4535.
16. Wu S, Sato M, Endo C, Sakurada A, Dong B, Aikawa H, Chen Y, Okada Y, Matsumura Y, Sueoka E, Kondo T: hnRNP B1 protein may be a possible prognostic factor in squamous cell carcinoma of the lung. Lung Cancer. 2003;41(2):179-186.
17. Cui H, Wu F, Sun Y, Fan G, Wang Q: Up-regulation and subcellular localization of hnRNP A2/B1 in the development of hepatocellular carcinoma. BMC Cancer. 2010;10:356.
18. Mizuno H, Honda M, Shirasaki T, Yamashita T, Yamashita T, Mizukoshi E, Kaneko S: Heterogeneous nuclear ribonucleoprotein A2/B1 in association with hTERT is a potential biomarker for hepatocellular carcinoma. Liver Int. 2012;32(7):1146-1155.
19. Jing GJ, Xu DH, Shi SL, Li QF, Wang SY, Wu FY, Kong HY: Aberrant expression and localization of hnRNP-A2/B1 is a common event in human gastric adenocarcinoma. J Gastroenterol Hepatol. 2011;26(1):108-115.
20. Sueoka E, Sueoka N, Iwanaga K, Sato A, Suga K, Hayashi S, Nagasawa K, Nakachi K: Detection of plasma hnRNP B1 mRNA, a new cancer biomarker, in lung cancer patients by quantitative real-time polymerase chain reaction. Lung Cancer. 2005;48(1):77-83.
21. Moran-Jones K, Grindlay J, Jones M, Smith R, Norman JC: hnRNP A2 regulates alternative mRNA splicing of TP53INP2 to control invasive cell migration. Cancer Res. 2009;69(24):9219-9227.
22. Clower CV, Chatterjee D, Wang Z, Cantley LC, Vander Heiden MG, Krainer AR: The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism. Proc Natl Acad Sci USA. 2010;107(5):1894-1899.
23. David CJ, Chen M, Assanah M, Canoll P, Manley JL: HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. 2010;463(7279):364-368.
24. Romero-Garcia S, Lopez-Gonzalez JS, Báez-Viveros JL, Aguilar-Cazares D, Prado-Garcia H: Tumor cell metabolism: an integral view. Cancer Biol Ther. 2011;12(11):939-948.
25. Garayoa M, Man YG, Mart́?nez A, Cuttitta F, Mulshine JL: Downregulation of hnRNP A2/B1 expression in tumor cells under prolonged hypoxia. Am J Respir Cell Mol Biol. 2003;28(1):80-85.
26. Lopez-Gonzalez JS, Prado-Garcia H, Aguilar-Cazares D, Molina-Guarneros JA, Morales-Fuentes J, Mandoki JJ: Apoptosis and cell cycle disturbances induced by coumarin and 7-hydroxycoumarin on human lung carcinoma cell lines. Lung Cancer. 2004;43(3):275-283.
27. Mane VP, Heuer MA, Hillyer P, Navarro MB, Rabin RL: Systematic method for determining an ideal housekeeping gene for real-time PCR analysis. J Biomol Tech. 2008;19(5): 342-347.
28. Soria S, de Anda R, Flores N, Romero-Garcia S, Gosset G, Boĺ?var F, Báez-Viveros JL: New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli. J Basic Microbiol. 2013;53(4):365-380.
29. Kozu T, Henrich B, Schäfer KP.: Structure and expression of the gene (HNRPA2B1) encoding the human hnRNP protein A2/B1. Genomics. 1995;25(2):365-371.
30. Pino I, Ṕ?o R, Toledo G, Zabalegui N, Vicent S, Rey N, Lozano MD, TorreW, Garć?a-Foncillas J,Montuenga LM: Altered patterns of expression of members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family in lung cancer. Lung Cancer. 2003;41(2):131-143.
31. Boukakis G, Patrinou-Georgoula M, Lekarakou M, Valavanis C, Guialis A: Deregulated expression of hnRNP A/B proteins in human non-small cell lung cancer: parallel assessment of protein and mRNA levels in paired tumour/non-tumour tissues. BMC Cancer. 2010;10:434.
32. Guha M, Pan H, Fang JK, Avadhani NG: Heterogeneous nuclear ribonucleoprotein A2 is a common transcriptional coactivator in the nuclear transcription response to mitochondrial respiratory stress. Mol Biol Cell. 2009;20(18): 4107-4119.
33. Golan-Gerstl R, CohenM, Shilo A, Suh SS, Bak̀acs A, Coppola L, Karni R: Splicing factor hnRNP A2/B1 regulates tumor suppressor gene splicing and is an oncogenic driver in glioblastoma. Cancer Res. 2011;71(13):4464-4472.
34. Tauler J, Zudaire E, Liu H, Shih J, Mulshine JL: hnRNP A2/B1 modulates epithelial-mesenchymal transition in lung cancer cell lines. Cancer Res. 2010;70(18):7137-7147.
35. Ye Q, Kantonen S, Gomez-Cambronero J: Serum deprivation confers the MDA-MB-231 breast cancer ine with an EGFR/JAK3/PLD2 system that maximizes cancer cell invasion. J Mol Biol. 2013;425(4) 755-766.