A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

Bioscience Reports

Volumn 33, Issue 6, 2013, Pages

  Chan, Weng Tat ^a   Verma, Chandra S ^a,b,c   Lane, David P ^c,d   Gan, Samuel Ken En ^a  

^a BIOINFORMATICS INSTITUTE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^d AGENCY FOR SCIENCE   (Singapore)

Author keywords

CaCl2 method; Competent bacteria; DMSO method; Hanahan's method; Heat shock

Indexed keywords

CALCIUM CHLORIDE; DIMETHYL SULFOXIDE; MAGNESIUM CHLORIDE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM COMPETENCE; BACTERIUM TRANSFORMATION; CULTURE MEDIUM; CULTURE OPTIMIZATION; CULTURE TECHNIQUE; ESCHERICHIA COLI; HANAHAN METHOD; HEAT SHOCK RESPONSE; INCUBATION TIME; INTERMETHOD COMPARISON; NONHUMAN;

CALCIUM CHLORIDE; CELL MEMBRANE PERMEABILITY; CULTURE MEDIA; DIMETHYL SULFOXIDE; DNA TRANSFORMATION COMPETENCE; ESCHERICHIA COLI; MAGNESIUM CHLORIDE; PLASMIDS; TRANSFORMATION, BACTERIAL;

EID: 84892586559     PISSN: 01448463     EISSN: 15734935     Source Type: Journal    
DOI: 10.1042/BSR20130098     Document Type: Article

References (16)

1. Griffith, F. (1928) The significance of pneumococcal types. J. Hyg. (Lond.). 27, 113-159
2. Michod, R. E., Wojciechowski, M. F. and Hoelzer, M. A. (1987) DNA repair and the evolution of transformation in the bacterium Bacillus subtilis. Genetics 118, 31-39
3. Bergmans, H. E., van Die, I. M. and Hoekstra, W. P. (1981) Transformation in Escherichia coli: stages in the process. J. Bacteriol. 146, 564-570
4. Mandel, M. and Higa, A. (1970) Calcium-dependent bacteriophage DNA infection. J. Mol. Biol. 53, 159-162
5. Cohen, S. N., Chang, A. C. and Hsu, L. (1972) Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. U. S. A. 69, 2110-2114
6. Weston, A., and Humphreys., G. O., Brown, M. G. and Saunders, J. R. (1979) Simultaneous transformation of Escherichia coli by pairs of compatible and incompatible plasmid DNA molecules. Mol. Gen. Genet. 172, 113-118
7. Roychoudhury, A., Basu, S. and Sengupta, D. N. (2009) Analysis of comparative efficiencies of different transformation methods of E. coli using two common plasmid vectors. Indian J. Biochem. Biophys. 46, 395-400
8. Li, X., Sui, X., Zhang, Y., Sun, Y., Zhao, Y., Zhai, Y. and Wang, Q. (2010) An improvised calcium chloride method preparation and transformation of competent cells. Afr. J. Biotechnol. 9, 8549-8554
9. Tu, Z., He, G., Li, K. X., and Chen., M. J., Chang, J., Chen, L., Yao, Q., and Liu., D. P., Ye, H., Shi, J. and Wu, X. (2005) An improvised system for competent cell preparation and high efficiency plasmid transformation using different Escherichia coli strains. Electron. J. Biotechnol. 8
10. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580
11. Hanahan, D., Jessee, J. and Bloom, F. R. (1991) Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol. 204, 63-113
12. Chung, C. T. and Miller, R. H. (1988) A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res. 16, 3580
13. Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: a Laboratory Manual, Cold Spring Harbor Laboratory Press, Volume 1
14. Bertani, G. (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62, 293-300
15. Chung, C. T., Niemela, S. L. and Miller, R. H. (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. U. S. A. 86, 2172-2175
16. Ryu, J. and Hartin, R. J. (1990) Quick transformation in Salmonella typhimurium LT2. BioTechniques 8, 43-45