Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Diagnostic Microbiology and Infectious Disease

Volumn 78, Issue 2, 2014, Pages 141-143

  Wu, Chi Jung ^a,b   Chen, Ya Ping ^b   Wang, Hsuan Chen ^a   Su, Ih Jen ^a   Ko, Wen Chien ^b   Chen, Jiann Shiuh ^b   Cheng, Chao Neng ^b   Lee, Nan Yao ^b   Sun, H Sunny ^c   Chi, Chia Yu ^a,b   Chen, Tsai Yun ^b  

^a NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

^b NATIONAL CHENG KUNG UNIVERSITY HOSPITAL   (Taiwan)

^c NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

Electrospray ionization mass spectrometry; Fungal infection; Paraffin; PCR

Indexed keywords

DIALYSIS FLUID;

ADOLESCENT; ADULT; AGED; ARTICLE; BIOPSY TECHNIQUE; BODY FLUID; CEREBROSPINAL FLUID; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FUNGAL DETECTION; FUNGUS CULTURE; FUNGUS IDENTIFICATION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LIVER BIOPSY; LUNG BIOPSY; LUNG LAVAGE; MIDDLE AGED; MYCOSIS; NONHUMAN; OROPHARYNX; PERITONEAL DIALYSIS; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; SKIN BIOPSY; SPECIES IDENTIFICATION; SPHENOID SINUS; SPUTUM; YOUNG ADULT;

ELECTROSPRAY IONIZATION MASS SPECTROMETRY; FUNGAL INFECTION; PARAFFIN; PCR;

ADOLESCENT; ADULT; AGED; CHILD; CHILD, PRESCHOOL; FUNGI; HUMANS; MIDDLE AGED; MULTILOCUS SEQUENCE TYPING; MYCOSES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; YOUNG ADULT;

EID: 84892482331     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2013.08.007     Document Type: Article

References (7)

1. Buitrago M.J., Aguado J.M., Ballen A., Bernal-Martinez L., Prieto M., Garcia-Reyne A., et al. Efficacy of DNA amplification in tissue biopsy samples to improve the detection of invasive fungal disease. Clin Microbiol Infect 2012, 19:E271-E277.
2. Cabaret O., Toussain G., Abermil N., Alsamad I.A., Botterel F., Costa J.M., et al. Degradation of fungal DNA in formalin-fixed paraffin-embedded sinus fungal balls hampers reliable sequence-based identification of fungi. Med Mycol 2011, 49:329-332.
3. De Castro N., Mazoyer E., Porcher R., Raffoux E., Suarez F., Ribaud P., et al. Hepatosplenic candidiasis in the era of new antifungal drugs: a study in Paris 2000-2007. Clin Microbiol Infect 2012, 18:E185-E187.
4. De Pauw B., Walsh T.J., Donnelly J.P., Stevens D.A., Edwards J.E., Calandra T., et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis 2008, 46:1813-1821.
5. Loeffler J., Henke N., Hebart H., Schmidt D., Hagmeyer L., Schumacher U., et al. Quantification of fungal DNA by using fluorescence resonance energy transfer and the Light Cycler system. J Clin Microbiol 2000, 38:586-590.
6. Manual of clinical microbiology 2010, ASM Press, Washington, DC. 10th ed.
7. Shin J.H., Ranken R., Sefers S.E., Lovari R., Quinn C.D., Meng S., et al. Detection, identification, and distribution of fungi in bronchoalveolar lavage specimens by use of multilocus PCR coupled with electrospray ionization/mass spectrometry. J Clin Microbiol 2013, 51:136-141.