Practical steady-state enzyme kinetics

Methods in Enzymology

Volumn 536, Issue , 2014, Pages 3-15

  Lorsch, Jon R ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Enzyme catalyzed reaction; Kinetic parameters determination; Michaelis Menten equation; Substrate concentration

Indexed keywords

AMINO ACID; CARBONATE DEHYDRATASE; ENZYME; ENZYME INHIBITOR; ISOMERASE;

CATALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; KINETICS; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; PROTEIN PROCESSING; REVIEW; SITE DIRECTED MUTAGENESIS; STEADY STATE;

ENZYME-CATALYZED REACTION; KINETIC PARAMETERS DETERMINATION; MICHAELIS-MENTEN EQUATION; SUBSTRATE CONCENTRATION;

ALGORITHMS; BIOCATALYSIS; ENZYME ASSAYS; ENZYME INHIBITORS; ENZYMES; KINETICS; MODELS, CHEMICAL; SUBSTRATE SPECIFICITY;

EID: 84892418797     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420070-8.00001-5     Document Type: Chapter

References (6)

1. A.R. Fresht Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding 1998 W.H. Freeman New York
2. K.A. Johnson Kinetic Analysis of Macromolecules: A Practical Approach 2003 Oxford University Press Oxford
3. Site-Directed Mutagenesis.
4. ATP and GTP hydrolysis assays (TLC).
5. Standard in vitro Assays for Protein-Nucleic Acid Interactions - Gel shift Assays for RNA and DNA Binding.
6. Protein Filter Binding.