Single cell cloning of a stable mammalian cell line

Methods in Enzymology

Volumn 536, Issue , 2014, Pages 165-172

  Longo, Patti A ^a   Kavran, Jennifer M ^a   Kim, Min Sung ^a   Leahy, Daniel J ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Clonal cell line; Dilution of cells; Protein expression; Single cell cloning; Stable mammalian cell line

Indexed keywords

ANAZOLENE SODIUM;

ARTICLE; CELL CLONING; CELL LINE; CELL POPULATION; CELL STRAIN HEK293; CHEMOLUMINESCENCE; CHO CELL; DILUTION; GENETIC TRANSFECTION; MAMMAL CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

CLONAL CELL LINE; DILUTION OF CELLS; PROTEIN EXPRESSION; SINGLE CELL CLONING; STABLE MAMMALIAN CELL LINE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; HEK293 CELLS; HUMANS; RECOMBINANT PROTEINS; SF9 CELLS; SPODOPTERA;

EID: 84892407378     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420070-8.00014-3     Document Type: Chapter

References (6)

1. Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® Recombination Cloning Technology and Flp-In T-REx® lines.
2. Generating mammalian stable cell lines by electroporation.
3. Lysis of mammalian and Sf9 cells.
4. One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE).
5. Coomassie Blue Staining.
6. Western Blotting using Chemiluminescent Substrates.