L-arginine does not improve biochemical and hormonal response in trained runners after 4 weeks of supplementation

Nutrition Research

Volumn 34, Issue 1, 2014, Pages 31-39

  Alvares, Thiago Silveira ^a   Conte Junior, Carlos Adam ^b   Silva, Joab Trajano ^a   Paschoalin, Vânia Margaret Flosi ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

Author keywords

Amino acids; Exercise; Healthy adult runners; Hormones; Nitric oxide; Nutritional supplements

Indexed keywords

AMMONIA; ARGININE; CYCLIC GMP; GROWTH HORMONE; HYDROCORTISONE; INSULIN; LACTIC ACID; NITRATE; NITRIC OXIDE; NITRITE; PLACEBO; SOMATOMEDIN C;

ARTICLE; BLOOD LEVEL; BLOOD SAMPLING; CONTROLLED STUDY; DOUBLE BLIND PROCEDURE; EXERCISE; FEMALE; HORMONE RESPONSE; HUMAN; HUMAN EXPERIMENT; MALE; METABOLISM; MOTOR PERFORMANCE; NORMAL HUMAN; PRIORITY JOURNAL; RANDOMIZED CONTROLLED TRIAL; RUNNING;

ELAGATIS;

+20; 5-KM TIME-TRIAL RUNNING TEST; 5KM-TT; ADMA; AFTER 20-MINUTE RECOVERY; AFTER 4 WEEKS; AFTER THE SECOND 5KM-TT; AMINO ACIDS; ARG; ASYMMETRIC DIMETHYLARGININE; BASELINE MEASUREMENTS; CGMP; CYCLIC GUANOSINE MONOPHOSPHATE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; ENOS; EXERCISE; FIRST 5KM-TT; GH; GROWTH HORMONE; HEALTHY ADULT RUNNERS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; HORMONES; HPLC; IGF-1; INSULIN-LIKE GROWTH FACTOR 1; IP-1; IP-2; L-ARGININE-SUPPLEMENTED GROUP; NITRATE PLUS NITRITE; NITRIC OXIDE; NO; NOX; NUTRITIONAL SUPPLEMENTS; PLA; PLACEBO GROUP; POST; PRE; TOTAL RUNNING TIME; TRT;

ADULT; AMMONIA; ARGININE; CYCLIC GMP; DIETARY SUPPLEMENTS; DOUBLE-BLIND METHOD; EXERCISE; FEMALE; HORMONES; HUMAN GROWTH HORMONE; HUMANS; HYDROCORTISONE; INSULIN; INSULIN-LIKE GROWTH FACTOR I; LACTIC ACID; MALE; NITRATES; NITRIC OXIDE; NITRITES; PLACEBOS; RUNNING;

EID: 84892370144     PISSN: 02715317     EISSN: 18790739     Source Type: Journal    
DOI: 10.1016/j.nutres.2013.10.006     Document Type: Article

References (34)

1. Alvares T.S., Meirelles C.M., Bhambhani Y.N., Paschoalin V.M., Gomes P.S. L-Arginine as a potential ergogenic aid in healthy subjects. Sports Med 2011, 41:233-248. 10.2165/11538590-000000000-00000.
2. Alvares T.S., Conte C.A., Paschoalin V.M., Silva J.T., Meirelles C.M., Bhambhani Y.N. Acute L-arginine supplementation increases muscle blood volume but not strength performance. Appl Physiol Nutr Metab 2012, 37:115-126. 10.1139/h11-144.
3. Giugliano D., Marfella R., Verrazzo G., Acampora R., Coppola L., Cozzolino D., D'Onofrio F. The vascular effects of L-arginine in humans. The role of endogenous insulin. J Clin Invest 1997, 99:433-438. 10.1172/JCI119177.
4. Chromiak J.A., Antonio J. Use of amino acids as growth hormone-releasing agents by athletes. Nutrition 2002, 18:657-661. 10.1016/S0899-9007(02)00807-9.
5. Collier S.R., Casey D.P., Kanaley J.A. Growth hormone responses to varying doses of oral arginine. Growth Horm IGF Res 2005, 15:136-139. 10.1016/j.ghir.2004.12.004.
6. Colombani P.C., Bitzi R., Frey-Rindova P., Frey W., Arnold M., Langhans W., Wenk C. Chronic arginine aspartate supplementation in runners reduces total plasma amino acid level at rest and during a marathon run. Eur J Nutr 1999, 38:263-270. 10.1007/s003940050076.
7. Forbes S.C., Bell G.J. The acute effects of a low and high dose of oral L-arginine supplementation in young active males at rest. Appl Physiol Nutr Metab 2011, 36:405-411. 10.1139/h11-035.
8. Forbes S.C., Harber V., Bell G.J. The acute effects of L-arginine on hormonal and metabolic responses during submaximal exercise in trained cyclists. Int J Sport Nutr Exerc Metab 2013, 23:369-377.
9. Santos R.S., Pacheco M.T.T., Martins R.A.B.L., Villaverde A.B., Giana H.E., Baptista F., Zângaro R.A. Study of the effect of oral administration of L-arginine on muscular performance in healthy volunteers. Isokinetic Exerc Sci 2002, 10:153-158.
10. Abel T., Knechtle B., Perret C., Eser P., von Arx P., Knecht H. Influence of chronic supplementation of arginine aspartate in endurance athletes on performance and substrate metabolism-a randomized, double-blind, placebo-controlled study. Int J Sports Med 2005, 26:344-349. 10.1055/s-2004-821111.
11. Burtscher M., Brunner F., Faulhaber M., Hotter B., Likar R. The prolonged intake of L-arginine-L-aspartate reduces blood lactate accumulation and oxygen consumption during submaximal exercise. J Sports Sci Med 2005, 4:314-322.
12. Campbell B., Roberts M., Kerksick C., Wilborn C., Marcello B., Taylor L., Nassar E., Leutholtz B., Bowden R., Rasmussen C., Greenwood M., Kreider R. Pharmacokinetics, safety, and effects on exercise performance of L-arginine alpha-ketoglutarate in trained adult men. Nutrition 2006, 22:872-881. 10.1016/j.nut.2006.06.003.
13. Little J.P., Forbes S.C., Candow D.G., Cornish S.M., Chilibeck P.D. Creatine, arginine alpha-ketoglutarate, amino acids, and medium-chain triglycerides and endurance and performance. Int J Sport Nutr Exerc Metab 2008, 18:493-508.
14. Fricke O., Baecker N., Heer M., Tutlewski B., Schoenau E. The effect of L-arginine administration on muscle force and power in postmenopausal women. Clin Physiol Funct Imaging 2008, 28:307-311. 10.1111/j.1475-097X.2008.00809.x.
15. Camic C.L., Housh T.J., Mielke M., Zuniga J.M., Hendrix C.R., Johnson G.O., Schmidt R.J., Housh D.J. The effects of 4 weeks of an arginine-based supplement on the gas exchange threshold and peak oxygen uptake. Appl Physiol Nutr Metab 2010, 35:286-293. 10.1139/H10-019.
16. Camic C.L., Housh T.J., Zuniga J.M., Hendrix R.C., Mielke M., Johnson G.O., Schmidt R.J. Effects of arginine-based supplements on the physical working capacity at the fatigue threshold. J Strength Cond Res 2010, 24:1306-1312. 10.1519/JSC.0b013e3181d68816.
17. Chen S., Kim W., Henning S.M., Carpenter C.L., Li Z. Arginine and antioxidant supplement on performance in elderly male cyclists: a randomized controlled trial. J Int Soc Sports Nutr 2010, 7:13-19. 10.1186/1550-2783-7-13.
18. Zajac A., Poprzecki S., Zebrowska A., Chalimoniuk M., Langfort J. Arginine and ornithine supplementation increases growth hormone and insulin-like growth factor-1 serum levels after heavy-resistance exercise in strength-trained athletes. J Strength Cond Res 2010, 24:1082-1090. 10.1519/JSC.0b013e3181d321ff.
19. Sunderland K.L., Greer F., Morales J. VO2max and ventilatory threshold of trained cyclists are not affected by 28-day L-arginine supplementation. J Strength Cond Res 2011, 25:833-837. 10.1519/JSC.0b013e3181c6a14d.
20. Willoughby D.S., Boucher T., Reid J., Skelton G., Clark M. Effects of 7 days of arginine-alpha-ketoglutarate supplementation on blood flow, plasma L-arginine, nitric oxide metabolites, and asymmetric dimethyl arginine after resistance exercise. Int J Sport Nutr Exerc Metab 2011, 21:291-299.
21. Alvares T.S., Conte-Junior C.A., Silva J.T., Flosi Paschoalin V.M. Acute L-Arginine supplementation does not increase nitric oxide production in healthy subjects. Nutr Metab (Lond) 2012, 9:54-61. 10.1186/1743-7075-9-54.
22. Wu G., Meininger C.J. Analysis of citrulline, arginine, and methylarginines using high-performance liquid chromatography. Methods Enzymol 2008, 440:177-189. 10.1016/S0076-6879(07)00810-5.
23. Bode-Böger S.M., Scalera F., Ignarro L.J. The L-arginine paradox: importance of the L-arginine/asymmetrical dimethylarginine ratio. Pharmacol Ther 2007, 114:295-306. 10.1016/j.pharmthera.2007.03.002.
24. Hov G.G., Sagen E., Bigonah A., Asberg A. Health-associated reference values for arginine, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) measured with high-performance liquid chromatography. Scand J Clin Lab Invest 2007, 67:868-876. 10.1080/00365510701429836.
25. Bode-Böger S.M., Böger R.H., Galland A., Tsikas D., Frölich J.C. L-arginine-induced vasodilation in healthy humans: pharmacokinetic-pharmacodynamic relationship. Br J Clin Pharmacol 1998, 46:489-497. 10.1046/j.1365-2125.1998.00803.x.
26. Balon T.W., Nadler J.L. Nitric oxide release is present from incubated skeletal muscle preparations. J Appl Physiol 1994, 77:2519-2521.
27. Mohr S., Stamler J.S., Brüne B. Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment. J Biol Chem 1996, 271:4209-4214. 10.1074/jbc.271.8.4209.
28. Pritzlaff C.J., Wideman L., Weltman J.Y., Abbott R.D., Gutgesell M.E., Hartman M.L., Veldhuis J.D., Weltman A. Impact of acute exercise intensity on pulsatile growth hormone release in men. J Appl Physiol 1999, 87:498-504.
29. Gordon S.E., Kraemer W.J., Vos N.H., Lynch J.M., Knuttgen H.G. Effect of acid-base balance on the growth hormone response to acute high-intensity cycle exercise. J Appl Physiol 1994, 76:821-829.
30. Matsumoto K., Mizuno M., Mizuno T., Dilling-Hansen B., Lahoz A., Bertelsen V., Münster H., Jordening H., Hamada K., Doi T. Branched-chain amino acids and arginine supplementation attenuates skeletal muscle proteolysis induced by moderate exercise in young individuals. Int J Sports Med 2007, 28:531-538. 10.1055/s-2007-964940.
31. Karkoulias K., Habeos I., Charokopos N., Tsiamita M., Mazarakis A., Pouli A., Spiropoulos K. Hormonal responses to marathon running in non-elite athletes. Eur J Intern Med 2008, 19:598-601. 10.1016/j.ejim.2007.06.032.
32. Dohm G.L., Kasperek G.J., Tapscott E.B., Beecher G.R. Effect of exercise on synthesis and degradation of muscle protein. Biochem J 1980, 188:255-262.
33. Smriga M., Ando T., Akutsu M., Furukawa Y., Miwa K., Morinaga Y. Oral treatment with L-lysine and L-arginine reduces anxiety and basal cortisol levels in healthy humans. Biomed Res 2007, 28:85-90. 10.2220/biomedres.28.85.
34. Eto B., Le Moel G., Porquet D., Peres G. Glutamate-arginine salts and hormonal responses to exercise. Arch Physiol Biochem 1995, 103:160-164. 10.3109/13813459508996129.