Cellulase activity screening using pure carboxymethylcellulose: Application to soluble cellulolytic samples and to plant tissue prints

International Journal of Molecular Sciences

Volumn 15, Issue 1, 2014, Pages 830-838

  Johnsen, Hanne R ^a   Krause, Kirsten ^a  

^a UNIVERSITY OF TROMSØ   (Norway)

Author keywords

Carboxymethylcellulose; Cellulase activity; Gram's iodine staining; Plant tissue prints

Indexed keywords

AMYLASE; CARBOXYMETHYLCELLULOSE; CELLULASE; PROTEINASE K; AGAR;

ARTICLE; ASPERGILLUS NIGER; CELL STRUCTURE; CONTROLLED STUDY; CUSCUTA REFLEXA; ENZYME ACTIVITY; ENZYME DEGRADATION; NONHUMAN; PLANT TISSUE CULTURE; SENSITIVITY AND SPECIFICITY; STAINING; TOMATO; ZYMOGRAPHY; CHEMISTRY; CUSCUTA; ENZYME ASSAY; ENZYME SPECIFICITY; METABOLISM; PELARGONIUM; PLANT; SOLANUM;

AGAR; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASE; CUSCUTA; ENZYME ASSAYS; PELARGONIUM; PLANTS; SOLANUM; SUBSTRATE SPECIFICITY;

EID: 84892170118     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms15010830     Document Type: Article

References (13)

1. Cai, S.; Li, J.; Hu, F.Z.; Zhang, K.; Luo, Y.; Janto, B.; Boissy, R.; Ehrlich, G.; Dong, X. Cellulosilyticum ruminicola, a newly described rumen bacterium that possesses redundant fibrolytic-protein-encoding genes and degrades lignocellulose with multiple carbohydrate-borne fibrolytic enzymes. Appl. Environ. Microbiol. 2010, 76, 3818-3824.
2. Kudo, T. Termite-microbe symbiotic system and its efficient degradation of lignocellulose. Biosci. Biotechnol. Biochem. 2009, 73, 2561-2567.
3. Vanholme, B.; de Meutter, J.; Tytgat, T.; van Montagu, M.; Coomans, A.; Gheysen, G. Secretions of plant-parasitic nematodes: A molecular update. Gene 2004, 332, 13-27.
4. Dashtban, M.; Schraft, H.; Qin, W. Fungal bioconversion of lignocellulosic residues; opportunities & perspectives. Int. J. Biol. Sci. 2009, 5, 578-595.
5. Lopez-Casado, G.; Urbanowicz, B.R.; Damasceno, C.M.B.; Rose, J.K.C. Plant glycosyl hydrolases and biofuels: A natural marriage. Curr. Opin. Plant Biol. 2008, 11, 329-337.
6. Percival Zhang, Y.H.; Hong, J.; Ye, X. Cellulase assays. Methods Mol. Biol. 2009, 581, 213-231.
7. Dashtban, M.; Maki, M.; Leung, K.T.; Mao, C.; Qin, W. Cellulase activities in biomass conversion: Measurement methods and comparison. Crit. Rev. Biotechnol. 2010, 30, 302-309.
8. Yeoh, H.H.; Khew, E.; Lim, G. A simple method for screening cellulolytic fungi. Mycologia 1985, 77, 161-162.
9. Kasana, R.C.; Salwan, R.; Dhar, H.; Dutt, S.; Gulati, A. A rapid and easy method for the detection of microbial cellulases on agar plates using Gram's iodine. Curr. Microbiol. 2008, 57, 503-507.
10. Zitomer, S.W.; Eveleigh, D.E. Cellulase screening by iodine staining: An artifact. Enzym. Microb. Technol. 1987, 9, 214-216.
11. Cassab, G.I.; Varner, J.E. Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper. J. Cell Biol. 1987, 105, 2581-2588.
12. Nagar, R.; Singh, M.; Sanwal, G.G. Cell-wall degrading enzymes in Cuscuta reflexa and its hosts. J. Exp. Bot. 1984, 35, 1104-1112.
13. Nagar, R.; Singh, M. Presence of a highly-active cellulase in Cuscuta reflexa. Indian J. Biochem. Biophys. 1980, 17, S96.