Evaluation of MALDI-TOF mass spectrometry and Sepsityper Kit™ for the direct identification of organisms from sterile body fluids in a Canadian pediatric hospital

Canadian Journal of Infectious Diseases and Medical Microbiology

Volumn 24, Issue 4, 2013, Pages 191-194

  Tadros, Manal ^a   Petrich, Astrid ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Blood culture analysis module; Blood cultures; Direct identification; MALDI TOF MS

Indexed keywords

ACINETOBACTER LWOFFII; ANALYTICAL EQUIPMENT; ARTICLE; BACTERIUM DETECTION; BLOOD CULTURE; CANADA; CEREBROSPINAL FLUID CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; GENUS; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROCOCCUS; NONHUMAN; PEDIATRIC HOSPITAL; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; SALMONELLA; SALMONELLA PARATYPHI; SERRATIA MARCESCENS; SPECIES IDENTIFICATION; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PARASANGUINIS; STREPTOCOCCUS PERORIS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS SALIVARIUS;

EID: 84892156326     PISSN: 17129532     EISSN: None     Source Type: Journal    
DOI: 10.1155/2013/701093     Document Type: Article

References (17)

1. Bamberger D. Diagnosis, initial management, and prevention of meningitis. Am Fam Physician 2010; 82:1491-8.
2. Kochanek K, Xu J, Murphy S, Miniño A, Kung H. Deaths: Preliminary data for 2009. National Vital Statistics Reports. Hyattsville, MD: National Center for Health Statistics. (Accessed April 11, 2011).
3. Kerremans J, Verboom P, Stijnen T, et al. Rapid identification and antimicrobial susceptibility testing reduce antibiotic use and accelerate pathogen-directed antibiotic use. J Antimicrob Chemother 2008; 61:428-35.
4. Kollef M. Broad-spectrum antimicrobials and the treatment of serious bacterial infections: Getting it right up front. Clin Infect Dis 2008; 47(Suppl 1):S3-13.
5. Seng P, Drancourt M, Gouriet F, et al. Ongoing revolution in bacteriology: Routine identification of bacteria by matrix assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009; 49:543-51.
6. Hsieh S, Tseng C, Lee Y, et al. Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS. Mol Cell Proteomics 2008; 7:448-56.
7. Murray P, Baron E, Jorgensen J, Marie, Landry M, Pfaller M. Manual of Clinical Microbiology, 9th edn. American Society for Microbiology. Washington, DC: ASM Press, 2007.
8. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697-703.
9. Janda J, Abbott S.16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: Pluses, perils, and pitfalls. J Clin Microbiol 2007; 45:2761-4.
10. Klein S, Zimmermann S, Köhler C, Mischnik A, Alle W, Bode K. Integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in blood culture diagnostics: A fast and effective approach. J Med Microbiol 2012; 61:323-31.
11. Sogawa K, Watanabe M, Sato K, et al. Use of the MALDI BioTyper system with MALDI-TOF mass spectrometry for rapid identification of microorganisms. Anal Bioanal Chem 2011; 400:1905-11.
12. Kok J, Thomas L, Olma T, Chen S, Iredell J. Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper™ and time of flight mass spectrometry. PLoS One 2011; 6:e23285.
13. Vlek A, Bonten M, Boel C. Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia. PLoS One 2012; 7:e32589.
14. Juiz P, Almela M, Melcion C, et al. A comparative study of two different methods of sample preparation for positive blood cultures for the rapid identification of bacteria using MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 2012; 31:1353-8.
15. Ferroni A, Saurez S, Beretti J, Dauphin B, Bille E, Meyer J. Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2010; 48:1542-8.
16. Warnero A, Christner M, Anderson T, Murdoch D. Differentiation of Streptococcus pneumoniae from nonpneumococcal streptococci of the Streptococcus mitis group by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2012; 50:2863-7.
17. He Y, Li H, Lu X, Stratton C, Tang Y. Mass spectrometry biotyper system identifies enteric bacterial pathogens directly from colonies grown on selective stool culture media. J Clin Microbiol 2010; 48:3888-92.