Recombinant serine racemase preparation

D-Amino Acids: A New Frontier in Amino Acid and Protein Research - Practical Methods and Protocols

Volumn , Issue , 2007, Pages 463-466

  Foltyn, Veronika N ^a   Wolosker, Herman ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

D serine; Pyridoxial 5 phosphate; Racemization; Recombinant; Serine racemase

Indexed keywords

EID: 84892044881     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (7)

1. De Miranda, J., Santoro, A., Engelender, S. & Wolosker, H. Human serine racemase: molecular cloning, genomic organization and functional analysis. Gene 256, 183-8. (2000).
2. Wolosker, H. et al. Purification of serine racemase: biosynthesis of the neuromodulator D-serine. Proc Natl Acad Sci U S A 96, 721-5. (1999).
3. Wolosker, H., Blackshaw, S. & Snyder, S. H. Serine racemase: a glial enzyme synthesizing D-serine to regulate glutamate-N-methyl-D-aspartate neurotransmission. Proc Natl Acad Sci U S A 96, 13409-14. (1999).
4. Wolosker, H., Panizzutti, R. & De Miranda, J. Neurobiology through the looking-glass: D-serine as a new glial-derived transmitter. Neurochem Int 41, 327. (2002).
5. Panizzutti, R., De Miranda, J., Ribeiro, C. S., Engelender, S. & Wolosker, H. A new strategy to decrease N-methyl-D-aspartate (NMDA) receptor coactivation: inhibition of D-serine synthesis by converting serine racemase into an eliminase. Proc Natl Acad Sci U S A 98, 5294-9. (2001).
6. De Miranda, J., Panizzutti, R., Foltyn, V. N. & Wolosker, H. Cofactors of serine racemase that physiologically stimulate the synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist Dserine. Proc Natl Acad Sci U S A 99, 14542-7 (2002).
7. Foltyn, V. N. et al. Serine racemase modulates intracellular D-serine levels through an alpha,betaelimination activity. J Biol Chem 280, 1754-63 (2005).