Molecular cloning and characterisation of metallothionein type 2a gene from Jatropha curcas L., a promising biofuel plant

Molecular Biology Reports

Volumn 41, Issue 1, 2014, Pages 113-124

  Mudalkar, Shalini ^a   Golla, Ramesh ^a   Sengupta, Debashree ^a   Ghatty, Sreenivas ^b   Reddy, Attipalli Ramachandra ^a  

^a UNIVERSITY OF HYDERABAD   (India)

^b Tree Oils India Limited   (India)

Author keywords

Cadmium; Copper; Heavy metals; Jatropha curcas L.; Metal homeostasis; Metallothionein; Phytoremediation

Indexed keywords

BIODIESEL; CADMIUM; COMPLEMENTARY DNA; COPPER; GLUTATHIONE TRANSFERASE; HEAVY METAL; METALLOTHIONEIN II; VEGETABLE OIL; ZINC;

ARTICLE; BIOFUEL PRODUCTION; CONTROLLED STUDY; ENERGY CROP; ENERGY DISPERSIVE X RAY SPECTROSCOPY; ESCHERICHIA COLI; FUNGUS MUTANT; GENE AMPLIFICATION; GENE ISOLATION; GENETIC ANALYSIS; GENOME SIZE; HETEROLOGOUS EXPRESSION; JATROPHA CURCAS; METAL TOLERANCE; METALLOTHIONEIN IIA GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYTOREMEDIATION; PLANT LEAF; PLANT ROOT; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RAPID AMPLIFICATION OF CDNA END; REAL TIME POLYMERASE CHAIN REACTION; SCANNING ELECTRON MICROSCOPY; SPECTROSCOPY; YEAST;

ESCHERICHIA COLI; JATROPHA CURCAS;

AMINO ACID SEQUENCE; BIODEGRADATION, ENVIRONMENTAL; BIOFUELS; CADMIUM CHLORIDE; CLONING, MOLECULAR; COPPER SULFATE; ENVIRONMENTAL POLLUTANTS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, PLANT; GENETIC COMPLEMENTATION TEST; JATROPHA; METALLOTHIONEIN; MICROBIAL VIABILITY; PLANT LEAVES; PLANT PROTEINS; PLANT ROOTS; SACCHAROMYCES CEREVISIAE; STRESS, PHYSIOLOGICAL;

EID: 84891953471     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-013-2843-5     Document Type: Article

References (77)

1. Cobbett CS, Goldsbrough PB (2000) Mechanisms of metal resistance: metallothioneins and phytochelatins. In: Raskin I, Ensley BD (eds) Phytoremediation of toxic metals: using plants to clean up the environment. Wiley, New York, pp 247-269
2. Clemens S (2006) Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88(11):1707-1719
3. Sheoran V, Sheoran AS, Poonia P (2011) Role of hyperaccumulators in phytoextraction of metals from contaminated mining sites: a review. Crit Rev Environ Sci Technol 41(2):168-214
4. Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1-11
5. Cobbett C, Goldsbrough P (2002) Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu Rev Plant Biol 53:159-182
6. Guo WJ, Bundithya W, Goldsbrough PB (2003) Characterization of the Arabidopsis metallothionein gene family: tissue-specific expression and induction during senescence and in response to copper. New Phytol 159:369-381
7. Domènech J, Mir G, Huguet G, Capdevila M, Molinas M et al (2006) Plant metallothionein domains: functional insight into physiological metal binding and protein folding. Biochimie 88:583-593
8. Domenech J, Orihuela R, Mir G, Molinas M, Atrian S et al (2007) The Cd(II)-binding abilities of recombinant Quercus suber metallothionein, QsMT: bridging the gap between phytochelatins and metallothioneins. J Biol Inorg Chem 12:867-882
9. Gonzalez-Mendoza D, Moreno AQ, Zapata-Perez O (2007) Coordinated responses of phytochelatin synthase and metallothionein genes in black mangrove, Avicennia germinans, exposed to cadmium and copper. Aquat Toxicol 83:306-314
10. Ferraz P, Fidalgo F, Almeida A, Teixeira J (2012) Phytostabilization of nickel by the zinc and cadmium hyperaccumulator Solanum nigrum L. Are metallothioneins involved? Plant Physiol Biochem 57:254-260
11. Palacios O, Atrian S, Capdevila M (2011) Zn- and Cu-thioneins: A functional classification for metallothioneins? J Biol Inorg Chem 16:991-1009
12. Domenech J, Tinti A, Torreggiani A (2007) Biopolymer research trends. In: Nemeth TS (ed) Research progress on metallothioneins: insights into structure, metal binding properties and molecular function by spectroscopic investigations. Nova Science Publishers, Inc., New York, pp 11-48
13. Zhou J, Goldsbrough PB (1994) Functional homologs of fungal metallothionein genes from Arabidopsis. Plant Cell 6:875-884
14. Robinson NJ, Tommey AM, Kuske C, Jackson PJ (1993) Plant metallothioneins. J Biochem 295:1-10
15. Freisinger E (2008) Plant MTs - long neglected members of the metallothionein superfamily. Dalton Trans 47:6663-6675
16. Freisinger E (2009) Metallothionein in plants. Met Ions Life Sci 5:107-153
17. Freisinger E (2011) Structural features specific to plant metallothioneins. J Biol Inorg Chem 16:1035-1045
18. Zhou J, Goldsbrough PB (1995) Structure, organization and expression of the metallothionein gene family in Arabidopsis. Mol Gen Genet 248:318-328
19. Zhou G, Xu Y, Li J, Yang L, Liu JY (2006) Molecular analyses of the metallothionein gene family in rice (Oryza sativa L.). J Biochem Mol Biol 39(5):595-606
20. Ma M, Lau PS, Jia YT, Tsang WK, Lam SKS et al (2003) The isolation and characterization of type1 metallothionein (MT) cDNA from a heavy-metal-tolerant plant Festuca rubra cv. Merlin. Plant Sci 164:51-60
21. Yang Z, Wu YR, Li Y, Ling HQ, Chu C (2009) OsMT1a, a type 1 metallothionein, plays the pivotal role in zinc homeostasis and drought tolerance in rice. Plant Mol Biol 70:219-229
22. Mir G, Domènech J, Huguet G, Guo WJ, Goldsbrough P et al (2004) A plant type 2 MT from cork tissue responds to oxidative stress. J Exp Bot 55:2483-2493
23. Zhigang A, Cuijie L, Yuangang Z, Yejie D, Wachter A et al (2006) Expression of BjMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis thaliana seedlings. J Exp Bot 57:3575-3582
24. Cozza R, Bruno L, Bitonti MB (2012) Expression pattern of a type-2 metallothionein gene in a wild population of the psammophyte Silene nicaeensis. Protoplasma. doi: 10.1007/s00709-012-0425-3
25. Zhang FQ, Wang YS, Sun CC, Lou ZP, Dong JD (2012) A novel metallothionein gene from a mangrove plant Kandelia candel. Ecotoxicology 21(6):1633-1641
26. Domenech J, Mir G, Huguet G, Capdevila M, Molinas M, Atrian S (2005) Plant metallothionein domains: functional insight into physiological metal binding and protein folding. Biochimie 88:583-593
27. Domenech J, Tinti A, Capdevila M, Atrian S, Torreggiani A (2007) Structural study of the zinc and cadmium complexes of a type 2 plant (Quercus suber) metallothionein: insights by vibrational spectroscopy. Biopolymers 86(3):240-248
28. Wan X, Freisinger E (2009) The plant metallothionein 2 from Cicer arietinum forms a single metal-thiolate cluster. Metallomics 1:489-500
29. Jordan RH, Turley RB, Defauw SL, Steele M (2005) Characterization of cDNA encoding metallothionein 3 from cotton (Gossypium hirsutum L.). DNA Seq 16(2):96-102
30. Freisinger E (2006) Spectroscopic characterization of a fruit-specific metallothionein: M. acuminata MT3. Inorgan Chim Acta 360(1):369-380
31. Rodríguez-Llorente ID, Pérez-Palacios P, Doukkali B, Caviedes MA, Pajuelo E (2010) Expression of the seed-specific metallothionein mt4a in plant vegetative tissues increases Cu and Zn tolerance. Plant Sci 178:327-332
32. Yuan J, Chen D, Ren Y, Zhang X, Zhao J (2008) Characteristic and expression analysis of a metallothionein gene, OsMT2b, downregulated by cytokinin suggests functions in root development and seed embryo germination of rice. J Plant Physiol 146:1637-1650
33. Lee J, Shim D, Song WY, Hwang I, Lee Y (2004) Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells. Plant Mol Biol 54:805-815
34. Kumar G, Kushwaha HR, Sabharwal VP et al (2012) Clustered metallothionein genes are co-regulated in rice and ectopic expression of OsMT1e-P confers multiple abiotic stress tolerance in tobacco via ROS scavenging. BMC Plant Biol 12:107
35. Hassinen V, Vallinkoski VM, Issakainen S, Tervahauta A, Karenlampi S, Servomaa K (2009) Correlation of foliar MT2b expression with Cd and Zn concentrations in hybrid aspen (Populus tremula × tremloides) grown in contaminated soil. Environ Poll 157:922-930
36. Hassinen VH, Tuomainen T, Peraniemi S, Karenlampi SO, Tervahauta AI (2009) Metallothionein 2 and 3 contribute to the metal adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlapsi caerulescens. J Exp Bot 60(1):187-196
37. Hassinen VH, Tervahauta AI, Schat H, Karenlampi SO (2011) Plant metallothioneins-metal chelators with ROS scavenging activity? Plant Biol 13:225-232
38. Akashi K, Nishimura N, Ishida Y, Yokota A (2004) Potent hydroxyl radical scavenging activity of drought-induced type-2 metallothionein in wild watermelon. Biochem Biophys Res Commun 323:72-78
39. Zhu W, Zhao DX, Miao Q, Xue TT, Li XZ, Zheng C (2009) Arabidopsis thaliana metallothionein, AtMT2a, mediates ROS balance during oxidative stress. J Plant Biol 52:585-592
40. Yang J, Wang Y, Liu G, Yang C, Li C (2011) Tamarix hispida metallothionein-like ThMT3, a reactive oxygen species scavenger, increases tolerance against Cd2+, Zn2+, Cu2+ and NaCl in transgenic yeast. Mol Biol Rep 38(3):1567-1574
41. Eswaran N, Parameswaran S, Sathram B, Anantharaman B, Raja KKG et al (2010) Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas. BMC Biotechnol 10:23
42. Yadav SK, Juwarkar AA, Phani KG, Thawale PR, Singh SK et al (2009) Bioaccumulation and phyto-translocation of arsenic, chromium and zinc by Jatropha curcas L.: impact of dairy sludge and bio fertilizer. Bioresour Technol 100(20):4616-4622
43. Gao S, Yan R, Cao M, Yang W, Wang S, Chen F (2008) Effects of copper on growth, antioxidant enzymes and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedling. Plant Soil Environ 54(3):117-122
44. Yan R, Gao S, Yang W, Cao M, Wang S et al (2008) Nickel toxicity induced antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. cotyledons. Plant Soil Environ 54:294-300
45. Mangkoedihardjo S, Surahmaida A (2008) Jatropha curcas L. for phytoremediation of lead and cadmium polluted soil. World Appl Sci J 4(4):519-522
46. Agamutu P, Abioye OP, Aziz AA (2010) Phytoremediation of soil contaminated with used lubricating oil using Jatropha curcas. J Hazard Mater 179:891-894
47. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
48. Longo VD, Gralla EB, Valentine JS (1996) Superoxide dismutase activity is essential for stationary phase survival in Sacchraromyces cerevisiae. J Biol Chem 271:12275-12280
49. -ΔΔCT method. Methods 25:402-408
50. Kuge S, Jones N (1994) YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides. EMBO J 13:655-664
51. Hamer DH, Thiele DJ, Lemontt JE (1985) Function and auto regulation of yeast copperthionein. Science 228:685-690
52. Usha B, Venkataraman G, Parida A (2009) Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro. Mol Genet Genomics 281:99-108
53. Zhou GK, Xu YF, Liu JY (2005) Characterization of a rice class II metallothionein gene: tissue expression patterns and induction in response to abiotic factors. J Plant Physiol 162:686-696
54. Rupesh KS, Sivalingam A, Shweta S, Vikas YP, Zakwan A et al (2011) Metallothionein-like gene from Cicer microphyllumis regulated by multiple abiotic stresses. Protoplasma 248:839-847
55. Hsieh HM, Huang PC (1998) Promoter structure and activity of type I rice metallothionein-like gene. Mitochondrial DNA 9(1):9-18
56. c-1 metallothionein: a metal-binding domain with a distinctive structure. J Mol Biol 387:207-218
57. c-1. J Inorg Biochem 103:342-353
58. c-1: the second part of the puzzle. J Biol Inorg Chem. doi: 10.1007/s00775-011-0770-2
59. Sekhar K, Priyanka B, Reddy VD, Rao KV (2011) Metallothionein 1 (CcMT1) of pigeonpea (Cajanus cajan L.) confers enhanced tolerance to copper and cadmium in Escherichia coli and Arabidopsis thaliana. Environ Exp Bot 72:131-139
60. Chaturvedi AK, Mishra A, Tiwari V, Jha B (2012) Cloning and transcript analysis of type 2 metallothionein gene (SbMT-2) from extreme halophyte Salicornia brachiata and its heterologous expression in E. coli. Gene 499:280-287
61. Guo GJ, Meetam M, Goldsbrough PB (2008) Examining the specific contributions of individual Arabidopsis metallothioneins to copper distribution and metal tolerance. Plant Physiol 146:1697-1706
62. Zhang YW, Tan NFY, Wong YS (2004) Cloning and characterization of type 2 metallothionein-like gene from a wetland plant Typha latifolia. Plant Sci 167:869-877
63. Van Hoof NALM, Hassinen VH, Hakvoort HWJ, Ballintijn KF, Schat H et al (2001) Enhanced copper tolerance in Silene vulgaris (Moench) Garcke populations from copper mines is associated with increased transcript levels of a 2b-type metallothionein gene. Plant Physiol 4:1519-1526
64. Chang T, Liu X, Xu H, Meng K, Chen S (2004) A metallothionein-like gene htMT2 strongly expressed in internodes and nodes of Helianthus tuberosa and effect of metal ion treatment on its expression. Planta 218:449-455
65. Kohler A, Blaudez D, Chalot M, Martin F (2004) Cloning and expression of multiple metallothioneins from hybrid poplar. New Phytol 164:83-93
66. Usha B, Prashanth SR, Parida A (2007) Differential expression of two metallothionein encoding genes during heavy metal stress in the mangrove species, Avicennia marina (Forsk.) Vierh. Curr Sci 93:1215-1219
67. Lacerda LD (1998) Biogeochemistry of trace metals and diffuse pollution in mangrove ecosystems. International Society for Mangrove Ecosystems, Okinawa 65
68. Olivares AR, Carillo-Gonzalez R, Gonzalez-Chavez MCA, Hernandez RMS (2013) Potential of castor bean (Ricinus communis L.) for phytoremediation of mine tailings and oil production. J Environ Manag 114:316-323
69. Mac Farlane GR, Burchett MD (2000) Cellular distribution of copper, lead, and zinc in the grey mangrove, Avicennia maria (Forsk.) Vierh. Aquat Bot 68:45-59
70. Kuzovkina YA, Knee M, Quigley MF (2004) Cadmium and copper uptake and translocation in five willow (Salix L.) species. Int J Phytoremediation 6(3):269-287
71. Belleghem FV, Cuypers A, Semane B, Smeets K, Vangronsveld J (2007) Subcellular localization of cadmium in roots and leaves of Arabidopsis thaliana. New Phytol 173:495-508
72. Rabier J, Laffont-Schwob I, Notonier R, Fogliani B, Bouraïma- Madjèbi S (2008) Anatomical element localization by EDXS in Grevillea exul var. exul under nickel stress. Environ Pollut 156:1156-1163
73. Bhargava A, Carmona FF, Bhargava M, Srivastava S (2012) Approaches for enhanced phytoextraction of heavy metals. J Environ Manag 105:103-120
74. Wagner GJ (1993) Accumulation of cadmium in crop plants and its consequences to human health. Adv Agron 51:173-213
75. Jamil S, Abhilash PC, Singh N, Sharma PN (2009) Jatropha curcas: a potential crop for phytoremediation of coal fly ash. J Hazard Mater 172:269-275
76. Jia D, Jing-Li Y, Cheng-Hao L (2012) Advances in metallothionein studies in forest trees. Plant Omics J 5(1):46-51
77. Gong JM, Lee DA, Schroeder JI (2003) Long distance root-to-shoot transport of phytochelatins and cadmium in plants. Proc Natl Acad Sci USA 100(17):10118-10123