Background free imaging of single mRNAs in live cells using split fluorescent proteins

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Wu, Bin ^a   Chen, Jiahao ^a   Singer, Robert H ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; PHOTOPROTEIN;

ARTICLE; BACTERIOPHAGE; FLUORESCENCE; GENETICS;

BACTERIOPHAGES; FLUORESCENCE; LUMINESCENT PROTEINS; RNA, MESSENGER;

EID: 84891933783     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep03615     Document Type: Article

References (12)

1. Bertrand, E. et al. Localization of ASH1mRNA particles in living yeast. Mol Cell 2, 437-445 (1998) (Pubitemid 128381296)
2. Wu, B., Chao, J. A. & Singer, R. H. Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells. Biophys J 102, 2936-2944 (2012)
3. Hu, C. D., Chinenov, Y. & Kerppola, T. K. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9, 789-798 (2002) (Pubitemid 34454889)
4. Rackham, O. & Brown, C. M. Visualization of RNA-protein interactions in living cells: FMRP and IMP1 interact on mRNAs. EMBO J 23, 3346-3355, doi:10.1038/ sj.emboj.7600341 (2004) (Pubitemid 39209158)
5. Valencia-Burton, M., McCullough, R. M., Cantor, C. R. & Broude, N. E. RNA visualization in live bacterial cells using fluorescent protein complementation. Nat Methods 4, 421-427 (2007) (Pubitemid 46901276)
6. Ozawa, T., Natori, Y., Sato, M. & Umezawa, Y. Imaging dynamics of endogenous mitochondrial RNA in single living cells. Nat Methods 4, 413-419 (2007) (Pubitemid 46901275)
7. Yiu, H.-W., Demidov, V. V., Toran, P., Cantor, C. R. & Broude, N. E. RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides. Pharmaceuticals 4, 494-508 (2011)
8. Chao, J. A., Patskovsky, Y., Almo, S. C. & Singer, R. H. Structural basis for the coevolution of a viral RNA-protein complex. Nat Struct Mol Biol 15, 103-105 (2008)
9. Hocine, S., Raymond, P., Zenklusen, D., Chao, J. A. & Singer, R. H. Singlemolecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods 10, 119-121 (2013)
10. Jaqaman, K. et al. Robust single-particle tracking in live-cell time-lapse sequences. Nat Methods 5, 695-702 (2008)
11. Fusco, D. et al. Single mRNA molecules demonstrate probabilistic movement in living mammalian cells. Curr Biol 13, 161-167 (2003) (Pubitemid 36154346)
12. Kerppola, T. K. Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells. Nat Protoc 1, 1278-1286 (2006)