GH52 xylosidase from Geobacillus stearothermophilus: Characterization and introduction of xylanase activity by site-directed mutagenesis of Tyr509

Journal of Industrial Microbiology and Biotechnology

Volumn 41, Issue 1, 2014, Pages 65-74

  Huang, Zongqing ^a   Liu, Xiaoshuang ^a   Zhang, Shaowei ^a   Liu, Ziduo ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Biotechnology; Geobacillus stearothermophilus; GH52 xylosidase; Protein engineering; Xylanase

Indexed keywords

AMINO ACID; ENZYME VARIANT; GLUTAMIC ACID; GLYCOSIDE HYDROLASE FAMILY 52 XYLOSIDASE; TYROSINE; UNCLASSIFIED DRUG; XYLAN; XYLAN 1,4 BETA XYLOSIDASE; XYLAN ENDO 1,3 BETA XYLOSIDASE; Y509E ENZYME;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; GEOBACILLUS STEAROTHERMOPHILUS; GSXYN GENE; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMINO ACID SEQUENCE; GEOBACILLUS STEAROTHERMOPHILUS; GLYCOSIDES; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; TYROSINE; XYLANS; XYLOSIDASES;

EID: 84891863154     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1351-x     Document Type: Article

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