Metabolic engineering of Corynebacterium glutamicum for 2-ketoisocaproate production

Applied Microbiology and Biotechnology

Volumn 98, Issue 1, 2014, Pages 297-311

  Bückle Vallant, Verena ^a   Krause, Felix S ^a   Messerschmidt, Sonja ^a   Eikmanns, Bernhard J ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

2 Ketoisocaproate production; Acetohydroxyacid synthase; Branched chain keto acids; Competitive inhibition; Corynebacterium glutamicum; Isopropylmalate synthase

Indexed keywords

ENCODING (SYMBOLS); ENZYME INHIBITION; GENES; GLUCOSE; METABOLIC ENGINEERING; SIGNAL ENCODING;

ACETOHYDROXYACID SYNTHASE; COMPETITIVE INHIBITION; CORYNEBACTERIUM GLUTAMICUM; KETO ACIDS; SYNTHASES;

SUBSTRATES;

2 ISOPROPYLMALATE DEHYDRATASE; 2 ISOPROPYLMALATE SYNTHASE; 2 OXOISOCAPROIC ACID; 2 OXOISOVALERIC ACID; ACETIC ACID; ACETOHYDROXYACID ISOMEROREDUCTASE; ACETOLACTATE SYNTHASE; BACTERIAL PROTEIN; BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE; CITRATE SYNTHASE; DIHYDROXYACID DEHYDRATASE; GLUCOSE; ILVE PROTEIN; LTBR PROTEIN; METHYLCITRATE SYNTHASE; UNCLASSIFIED DRUG;

ALLELE; AMINO ACID; BACTERIUM; BIOENGINEERING; BIOTECHNOLOGY; CARBOXYLIC ACID; DRUG DEVELOPMENT; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; GLUCOSE; INDUSTRIAL PRODUCTION; INHIBITION; METABOLISM; PRODUCTIVITY;

ALLELE; ARTICLE; BACTERIAL STRAIN; COMPETITIVE INHIBITION; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; FERMENTATION; GENE DELETION; GENE OVEREXPRESSION; METABOLIC ENGINEERING; TRANSCRIPTION REGULATION;

CORYNEBACTERIUM GLUTAMICUM;

ACETATES; CORYNEBACTERIUM GLUTAMICUM; GENE DELETION; GENE EXPRESSION; GLUCOSE; KETO ACIDS; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS;

EID: 84891859051     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5310-2     Document Type: Article

References (84)

1. Abe S, Takayama K, Kinoshita S (1967) Taxonomical studies on glutamic acid-producing bacteria. J Gen Appl Microbiol 13:279-301
2. Aparicio M, Cano NJM, Cupisti A, Ecder T, Fouque D, Garneata L, Liou H, Lin S, Schober-Halstenberg H, Teplan V, Zakar G (2009) Keto-acid therapy in predialysis chronic kidney disease patients: consensus statements. J Ren Nutr 19:33-35
3. Arndt A, Auchter M, Ishige T, Wendisch VF, Eikmanns BJ (2008) Ethanol catabolism in Corynebacterium glutamicum. J Mol Microbiol Biotechnol 15:222-233
4. Bigelis R, Umbarger HE (1976) Yeast alpha-isopropylmalate isomerase. Factors affecting stability and enzyme activity. J Biol Chem 251:3545-3552
5. Birnboim HC (1983) A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol 100:243-255
6. Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ (2008) Corynebacterium glutamicum tailored for high-yield L-valine production. Appl Microbiol Biotechnol 79:471-479
7. Blombach B, Stephan H, Brigitte B, Eikmanns BJ (2009) Acetohydroxyacid synthase, a novel target for improvement of L-lysine production by Corynebacterium glutamicum. Appl Environ Microbiol 75:419-427
8. Blombach B, Riester T, Wieschalka S, Ziert C, Youn JW, Wendisch VF, Eikmanns BJ (2011) Corynebacterium glutamicum tailored for efficient isobutanol production. Appl Environ Microbiol 77:3300-3310
9. Brune I, Jochmann N, Brinkrolf K, Hüser AT, Gerstmeir R, Eikmanns BJ, Kalinowski J, Pühler A, Tauch A (2007) The IclR-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum. J Bacteriol 189:2720-2733
10. Chang JH, Kim DK, Park JT, Kang EW, Yoo TH, Kim BS, Choi KH, Lee HY, Han D, Shin SK (2009) Influence of ketoanalogs supplementation on the progression in chronic kidney disease patients who had training on low-protein diet. Nephrology 14:750-757
11. Connor MR, Liao JC (2008) Engineering of an Escherichia coli strain for the production of 3-methyl-1-butanol. Appl Environ Microbiol 74:5769-5775
12. Connor MR, Cann AF, Liao JC (2010) 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl Microbiol Biotechnol 86:1155-1164
13. Cooper AJL, Ginos JZ, Meister A (1983) Synthesis and properties of the alpha-keto acids. Chem Rev 83:321-358
14. Dower WJ, Miller JF, Ragsdale CW (1988) High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res 16:6127-6145
15. Eggeling L, Bott M (2005) Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, pp 13-16
16. Eggeling I, Cordes C, Eggeling L, Sahm H (1987) Regulation of acetohydroxy acid synthase in Corynebacterium glutamicum during fermentation of 2-ketobutyrate to L-isoleucine. Appl Microbiol Biotechnol 25:346-351
17. Eikmanns BJ, Metzger M, Reinscheid D, Kircher M, Sahm H (1991) Amplification of three threonine biosynthesis genes in Corynebacterium glutamicum and its influence on carbon flux in different strains. Appl Microbiol Biotechnol 34:617-622
18. Eikmanns BJ, Thum-Schmitz N, Eggeling L, Lüdtke KU, Sahm H (1994) Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase. Microbiology 140:1817-1828
19. Feiten SF, Draibe SA, Watanabe R, Duenhas MR, Baxmann AC, Nerbass FB, Cuppari L (2005) Short-term effects of a very-low-protein diet supplemented with ketoacids in nondialyzed chronic kidney disease patients. Eur J Clin Nutr 59:129-136
20. Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ (2003) Acetate metabolism and its regulation in Corynebacterium glutamicum. J Biotechnol 104:99-122
21. Gross SR (1965) The regulation of synthesis of leucine biosynthetic enzymes in Neurospora. Proc Natl Acad Sci U S A 54:1538-1546
22. Gusyatiner MM, Lunts MG, Kozlov YI, Ivanovskaya LV, Voroshilova EB (2002) DNA coding for mutant isopropylmalate synthase L-leucine-producing microorganism and method for producing L-leucine. US patent 6,403,342 B1
23. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
24. Hasegawa S, Suda M, Uematsu K, Natsuma Y, Hiraga K, Jojima T, Inui M, Yukawa H (2013) Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions. Appl Environ Microbiol 79:1250-1257
25. Heider SAE, Peters-Wendisch P, Wendisch VF (2012) Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum. BMC Microbiol 12:98
26. Hsu Y-P, Kohlhaw GB, Niederberger P (1982) Evidence that alpha-isopropylmalate synthase of Saccharomyces cerevisiae is under the "general" control of amino acid biosynthesis. J Bacteriol 150:969-972
27. Hüser AT, Chassagnole C, Lindley ND, Merkamm M, Guyonvarch A, Elisáková V, Pátek M, Kalinowski J, Brune I, Pühler A, Tauch A (2005) Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling. Appl Environ Microbiol 71:3255-3268
28. Ikeda M, Mitsuhashi S, Tanaka K, Hayashi M (2009) Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl Environ Microbiol 75:1635-1641
29. Inui M, Murakami S, Okino S, Kawaguchi H, Vertès AA, Yukawa H (2004a) Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions. J Mol Microbiol Biotechnol 7:182-196
30. Inui M, Kawaguchi H, Murakami S, Vertès AA, Yukawa H (2004b) Metabolic engineering of Corynebacterium glutamicum for fuel ethanol production under oxygen deprivation-conditions. J Mol Microbiol Biotechnol 8:243-254
31. Jensen JVK, Wendisch VF (2013) Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum. Microb Cell Fact 12:63
32. Jo S, Maeda M, Ooi T, Taguchi S (2006) Production system for biodegradable polyester polyhydroxybutyrate by Corynebacterium glutamicum. J Biosci Bioeng 102:233-236
33. Jo JH, Seol HY, Lee YB, Kim MH, Hyun HH, Lee HH (2012) Disruption of genes for the enhanced biosynthesis of α-ketoglutarate in Corynebacterium glutamicum. Can J Microbiol 58:278-286
34. Kind S, Wittmann C (2011) Bio-based production of the platform chemical 1,5 diaminopentane. Appl Microbiol Biotechnol 5:1287-1296
35. Kinoshita S, Udaka S, Shimono M (1957) Studies on the amino acid fermentation. Production of L-glutamic acid by various microorganisms. J Gen Appl Microbiol 3:193-205
36. Kohlhaw GB (1988) Alpha-isopropylmalate synthase from yeast. Methods Enzymol 166:414-423
37. Kohlhaw GB (2003) Leucine biosynthesis in fungi: entering metabolism through the back door. Microbiol Mol Biol Rev 67:1-15
38. Kohlhaw GB, Leary TR, Umbarger HE (1969) Alpha-isopropylmalate synthase from Salmonella typhimurium: purification and properties. J Biol Chem 244:2218-2225
39. Krause FS, Blombach B, Eikmanns BJ (2010a) Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production. Appl Environ Microbiol 76:8053-8061
40. Krause FS, Henrich A, Blombach B, Krämer R, Eikmanns BJ, Seibold GM (2010b) Increased glucose utilization in Corynebacterium glutamicum by use of maltose, and its application for the improvement of L-valine productivity. Appl Environ Microbiol 76:370-374
41. Krill S (2003) Verfahren zur Herstellung von 6-Methylheptanon. European patent 1366009 A1
42. Lee EH, Cho YW, Hwang KY (2012) Crystal structure of LeuD from Methanococcus jannaschii. Biochem Biophys Res Commun 419:160-164
43. Liebl W (2006) The genus Corynebacterium - nonmedical. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The Prokaryotes, vol 3, 3rd edn. Springer, New York, pp 796-818
44. Litsanov B, Brocker M, Bott M (2012a) Towards homosuccinate fermentation: metabolic engineering of Corynebacterium glutamicum for anaerobic succinate production from glucose and formate. Appl Environ Microbiol 78:3325-3337
45. Litsanov B, Kabus A, Brocker M, Bott M (2012b) Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum. Microb Biotechnol 5:116-128
46. Marienhagen J, Eggeling L (2008) Metabolic function of Corynebacterium glutamicum aminotransferases AlaT and AvtA and impact on L-valine production. Appl Environ Microbiol 74:7457-7462
47. Marienhagen J, Kennerknecht N, Sahm H, Eggeling L (2005) Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum. J Bacteriol 187:7639-7646
48. Mimitsuka T, Sawai H, Hatsu M, Yamada K (2007) Metabolic engineering of Corynebacterium glutamicum for cadaverin fermentation. Biosci Biotechnol Biochem 71:2130-2135
49. Morbach S, Junger C, Sahm H, Eggeling L (2000) Attenuation control of ilvBNC in Corynebacterium glutamicum: evidence of leader peptide formation without the presence of a ribosome binding site. J Biosci Bioeng 90:501-507
50. Nishimura T, Vertès AA, Shinoda Y, Inui M, Yukawa H (2007) Anaerobic growth of Corynebacterium glutamicum using nitrate as a terminal electron acceptor. Appl Microbiol Biotechnol 75:889-897
51. Nunan D, Howatson G, van Someren KA (2010) Exercise-induced muscle damage is not attenuated by beta-hydroxy-beta-methylbutyrate and alpha-ketoisocaproic acid supplementation. J strength Cond Res 24:531-537
52. Okino S, Suda M, Fujikura K, Inui M, Yukawa H (2008) Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 78:449-454
53. Parsons SJ, Burns OR (1969) Purification and properties of β-isopropylmalate dehydrogenase. J Biol Chem 244:996-1003
54. Pátek M (2007) Branched-chain amino acids. In: Wendisch VF (ed) Amino acid biosynthesis - pathways, regulation and metabolic engineering. Springer, Berlin, pp 129-162
55. Pátek M, Krumbach K, Eggeling L, Sahm H (1994) Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine synthesis. Appl Environ Microbiol 60:133-140
56. Pátek M, Hochmannová J, Jelínková M, Nesvera J, Eggeling L (1998) Analysis of the leuB gene from Corynebacterium glutamicum. Appl Microbiol Biotechnol 50:42-47
57. Radmacher E, Eggeling L (2007) The three tricarboxylate synthase activities of Corynebacterium glutamicum and increase of L-lysine synthesis. Appl Microbiol Biotechnol 76:587-595
58. Sahm H, Eggeling L (1999) d-Pantothenate synthesis in Corynebacterium glutamicum and use of panBC and genes encoding l-valine synthesis for d-pantothenate overproduction. Appl Environ Microbiol 65:1973-1979
59. Sakai S, Tsuchida Y, Nakamoto H, Okino S, Ichihashi O, Kawaguchi H, Watanabe T, Inui M, Yukawa H (2007) Effect of lignocellulose-derived inhibitors on growth of and ethanol production by growth-arrested Corynebacterium glutamicum R. Appl Environ Microbiol 73:2349-2353
60. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, vol 73, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 2349-2353
61. Sasaki M, Jojima T, Inui M, Yukawa H (2010) Xylitol production by recombinant Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 86:1057-1066
62. Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A (1994) Small mobilizable multi-purpose cloning vectors derived from the E. coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73
63. Schneider J, Wendisch VF (2010) Putrescine production by engineered Corynebacterium glutamicum. Appl Microbiol Biotechnol 88:859-868
64. Schneider J, Wendisch VF (2011) Biotechnological production of polyamines by bacteria: recent achievements and future perspectives. Appl Microbiol Biotechnol 91:17-30
65. Schrumpf B, Eggeling L, Sahm H (1992) Isolation and prominent characteristics of anL-lysine hyperproducing strain of Corynebacterium glutamicum. Appl Microbiol Biotechnol 37:566-571
66. Seliverstov AV, Putzer H, Gelfand MS, Lyubetsky VA (2005) Comparative analysis of RNA regulatory elements of amino acid metabolism genes in Actinobacteria. BMC Microbiol 5:54
67. Srere PA (1969) Citrate synthase. Methods Enzymol 13:3-11
68. Stieglitz BI, Calvo JM (1974) Distribution of the isopropylmalate pathway to leucine among diverse bacteria. J Bacteriol 118:935-994
69. Takeno S, Ohnishi J, Komatsu T, Masaki T, Sen K, Ikeda M (2007) Anaerobic growth and potential for amino acid production by nitrate respiration in Corynebacterium glutamicum. Appl Microbiol Biotechnol 75:1173-1182
70. Takors R, Bathe B, Rieping M, Hans S, Kelle R, Huthmacher K (2007) Systems biology for industrial strains and fermentation processes - example: amino acids. J Biotechnol 129:181-190
71. Teschan PE, Beck GJ, Dwyer JT, Greene T, Klahr S, Levy AS, Mitch WE, Snetselaar LG, Steinman TI, Walser M (1998) Effect of a ketoacid-aminoacid supplemented very low protein diet on the progression of advanced renal disease: a reanalysis of the MDRD feasibility study. Clin Nephrol 50:273-283
72. Teschner M, Heidland A (1991) Nutritional management of the uremic patient. In: Suki WN, Massry SG (eds) Therapy of renal diseases and related disorders, 2nd edn. Kluwer Academic Publishers, Norwell, pp 675-696
73. van der Rest ME, Lange C, Molenaar D (1999) A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogenic plasmid DNA. Appl Microbiol Biotechnol 52:541-545
74. van Ooyen J, Noack S, Bott M, Reth A, Eggeling L (2012) Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. Biotechnol Bioeng 109:2070-2081
75. van Someren KA, Edwards AJ, Howatson G (2005) Supplementation with betahydroxy-beta-methylbutyrate (HMB) and alpha-ketoisocaproic acid (KIC) reduces signs and symptoms of exercise-induced muscle damage in man. Int J Sport Nutr Exerc Metab 15:413-424
76. Vásicová P, Pátek M, Nesvera J, Sahm H, Eikmanns BJ (1999) Analysis of the Corynebacterium glutamicum dapA promoter. J Bacteriol 181:6188-6191
77. Wendisch VF, Spies M, Reinscheid DJ, Schnicke S, Sahm H, Eikmanns BJ (1997) Regulation of acetate metabolism in Corynebacterium glutamicum: transcriptional control of the isocitrate lyase and malate synthase genes. Arch Microbiol 168:262-269
78. Wiegel J, Schlegel HG (1977a) α-Isopropylmalate synthase from Alcaligenes eutrophus H 16. II. Substrate specificity and kinetics. Arch Microbiol 112:247-254
79. Wiegel J, Schlegel HG (1977b) α-Isopropylmalate synthase from Alcaligenes eutrophus H 16. III. End product inhibition and its relief by valine and isoleucine. Arch Microbiol 114:203-210
80. Wieschalka S, Blombach B, Eikmanns BJ (2012) Engineering Corynebacterium glutamicum for the production of pyruvate. Appl Microbiol Biotechnol 94:449-459
81. Wieschalka S, Blombach B, Bott M, Eikmanns BJ (2013) Bio-based production of organic acids with Corynebacterium glutamicum. Microb Biotechnol 6:87-102
82. Yamamoto S, Suda M, Niimi S, Inui M, Yukawa H (2013) Strain optimization for efficient isobutanol production using Corynebacterium glutamicum under oxygen deprivation. Biotechnol Bioeng. doi: 10.1002/bit.24961
83. Yindeeyoungyeon W, Likitvivatanavong S, Palittapongarnpim P (2009) Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis. BMC Microbiol 9:122
84. Zanchi NE, Gerlinger-Romero F, Guimarães-Ferreira L, de Siqueira Filho MA, Felitti V, Lira FS, Seelaender M, Lancha AH Jr (2011) HMB supplementation: clinical and athletic performance-related effects and mechanisms of action. Amino Acids 40:1015-1025