TAL effector specificity for base 0 of the DNA target is altered in a complex, effector- and assay-dependent manner by substitutions for the tryptophan in cryptic repeat -1

PLoS ONE

Volumn 8, Issue 12, 2013, Pages

  Doyle, Erin L ^a,f   Hummel, Aaron W ^a   Demorest, Zachary L ^b,c   Starker, Colby G ^b   Voytas, Daniel F ^b   Bradley, Philip ^d   Bogdanove, Adam J ^a,e  

^a IOWA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^c Arvid Wallgrens Backe 20   (United States)

^d Fred Hutchinson Cancer Research Center   (United States)

^e Department of Obstetrics Gynecology   (United States)

^f Doane College   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; GUANINE; HYBRID PROTEIN; NUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE FUSION PROTEIN; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR PTHXO1; TRANSCRIPTION FACTOR; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA BINDING; DNA CLEAVAGE; DNA SEQUENCE; ENZYME ACTIVE SITE; GENE; GENE CONSTRUCT; GENE TARGETING; GENETIC ENGINEERING; GENETIC VARIABILITY; LONG TERMINAL REPEAT; NONHUMAN; PROTEIN FUNCTION; PTHXO1 GENE; RALSTONIA SOLANACEARUM; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; WILD TYPE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; DNA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN CONFORMATION; RALSTONIA SOLANACEARUM; REPETITIVE SEQUENCES, AMINO ACID; SUBSTRATE SPECIFICITY; TRANSCRIPTION FACTORS; TRYPTOPHAN;

EID: 84891819652     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0082120     Document Type: Article

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