Assay methods for small ubiquitin-like modifier (SUMO)-SUMO-interacting motif (SIM) interactions in vivo and in vitro using a split-luciferase complementation system

Analytical Biochemistry

Volumn 448, Issue 1, 2014, Pages 92-94

  Hirohama, Mikako ^a,c,e   Voet, Arnout R D ^a   Ozawa, Takeaki ^d   Saitoh, Hisato ^b   Nakao, Yoichi ^e   Zhang, Kam Y J ^a   Ito, Akihiro ^a   Yoshida, Minoru ^a,c  

^a Elements Chemistry Laboratory   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

^e WASEDA UNIVERSITY   (Japan)

Author keywords

Assay; Protein protein interactions; SIM; SUMOylation

Indexed keywords

ASSAYS; IONS; SECONDARY ION MASS SPECTROMETRY;

BACTERIAL EXPRESSION SYSTEMS; HIGH THROUGHPUT; MULTIPLE CELLULAR PROCESS; NON-COVALENT INTERACTION; PROTEIN-PROTEIN INTERACTIONS; SMALL UBIQUITIN-LIKE MODIFIERS; SUMOYLATION; TARGET PROTEINS;

PROTEINS;

RENILLA LUCIFERIN 2 MONOOXYGENASE; SUMO INTERACTING MOTIF; SUMO PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; GENE EXPRESSION SYSTEM; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SUMOYLATION;

ASSAY; PROTEIN-PROTEIN INTERACTIONS; SIM; SUMOYLATION;

AMINO ACID SEQUENCE; CARRIER PROTEINS; ESCHERICHIA COLI; FLUORESCENT DYES; GENES, REPORTER; HUMANS; LIGHT; LUCIFERASES; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; SUMO-1 PROTEIN;

EID: 84891816010     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.12.009     Document Type: Article

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