Human dental pulp stem cells cultured in serum-free supplemented medium

Frontiers in Physiology

Volumn 4 DEC, Issue , 2013, Pages

  Bonnamain, Virginie ^a   Thinard, Reynald ^a   Sergent Tanguy, Solène ^a   Huet, Pascal ^b   Bienvenu, Géraldine ^b   Naveilhan, Philippe ^a   Farges, Jean Christophe ^c   Alliot Licht, Brigitte ^a,b  

^a Centre National de la Recherche Scientifique   (France)

^b UNIVERSITÉ DE NANTES   (France)

^c Univ Lyon 1   (France)

Author keywords

Human dental pulp stem cells; Neurogenic serum free medium; Neuronal progenitor; Odontoblastic differentiation; Oligodendrocyte progenitor

Indexed keywords

BETA TUBULIN; CD133 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; CD56 ANTIGEN; CD71 ANTIGEN; CHEMOKINE RECEPTOR CXCR3; EPIDERMAL GROWTH FACTOR; FIBROBLAST GROWTH FACTOR 2; GLIAL FIBRILLARY ACIDIC PROTEIN; GREEN FLUORESCENT PROTEIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; NESTIN; OSTEOCALCIN; THY 1 ANTIGEN; TRANSFERRIN RECEPTOR;

ADOLESCENT; ADULT; ARTICLE; ASTROCYTE; CLINICAL ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HUMAN; IMMUNOPHENOTYPING; MALE; MESENCHYME CELL; ODONTOBLAST; OLIGODENDROGLIA; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; TOOTH PULP;

EID: 84891682235     PISSN: None     EISSN: 1664042X     Source Type: Journal    
DOI: 10.3389/fphys.2013.00357     Document Type: Article

References (38)

1. Abe, S., Hamada, K., Miura, M., and Yamaguchi, S. (2012). Neural crest stem cell property of apical pulp cells derived from human developing tooth. Cell Biol. Int. 36, 927-936. doi: 10.1042/CBI20110506.
2. Adachi, T., Nakanishi, T., Yumoto, H., Hirao, K., Takahashi, K., Mukai, K., et al. (2007). Caries-related bacteria and cytokines induce CXCL10 in dental pulp. J. Dent. Res. 86, 1217-1222. doi: 10.1177/154405910708601215.
3. Alipour, R., Sadeghi, F., Hashemi-Beni, B., Zarkesh-Esfahani, SH., Heydari, F., Mousavi, S. B., et al. (2010). Phenotypic characterizations and comparison of adult dental stem cells with adipose-derived stem cells. Int. J. Prev. Med. 1, 164-171.
4. Alliot-Licht, B., Bluteau, G., Magne, D., Lopez-Cazaux, S., Lieubeau, B., Daculsi, G., et al. (2005). Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures. Cell Tissue Res. 321, 391-400. doi: 10.1007/s00441-005-1115-7.
5. Arthur, A., Rychkov, G., Shi, S., Koblar, S. A., and Gronthos, S. (2008). Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. Stem Cells 26, 1787-1795. doi: 10.1634/stemcells.2007-0979.
6. Arthur, A., Shi, S., Zannettino, A. C., Fujii, N., Gronthos, S., and Koblar, S. A. (2009). Implanted adult human dental pulp stem cells induce endogenous axon guidance. Stem Cells 27, 2229-2237. doi: 10.1002/stem.138.
7. Bonnamain, V., Mathieux, E., Thinard, R., Thebault, P., Nerriere-Daguin, V., Leveque, X., et al. (2012). Expression of heme oxygenase-1 in neural stem/progenitor cells as a potential mechanism to evade host immune response. Stem Cells 30, 2342-2353. doi: 10.1002/stem.1199.
8. Bonnamain, V., Neveu, I., and Naveilhan, P. (2011). In vitro analyses of the immunosuppressive properties of neural stem/progenitor cells using anti-CD3/CD28-activated T cells. Methods Mol. Biol. 677, 233-343. doi: 10.1007/978-1-60761-869-0_17.
9. Caldwell, M. A., Garcion, E., ter Borg, M. G., He, X., and Svendsen, C. N. (2004). Heparin stabilizes FGF-2 and modulates striatal precursor cell behavior in response to EGF. Exp. Neurol. 188, 408-420. doi: 10.1016/j.expneurol.2004.05.007 S0014488604001797.
10. Couble, M. L., Farges, J. C., Bleicher, F., Perrat-Mabillon, B., Boudeulle, M., and Magloire, H. (2000). Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif. Tissue Int. 66, 129-138. doi: 10.1007/PL00005833.
11. d'Aquino, R., Tirino, V., Desiderio, V., Studer, M., De Angelis, G. C., Laino, L., et al. (2011). Human neural crest-derived postnatal cells exhibit remarkable embryonic attributes either in vitro or in vivo. Eur. Cell. Mater. 21, 304-316.
12. Ferro, F., Spelat, R., Beltrami, A. P., Cesselli, D., and Curcio, F. (2012). Isolation and characterization of human dental pulp derived stem cells by using media containing low human serum percentage as clinical grade substitutes for bovine serum. PLoS ONE 7:e48945. doi: 10.1371/journal.pone.0048945.
13. Gregory, C. A., Reyes, E., Whitney, M. J., and Spees, J. L. (2006). Enhanced engraftment of mesenchymal stem cells in a cutaneous wound model by culture in allogenic species-specific serum and administration in fibrin constructs. Stem Cells 24, 2232-2243. doi: 10.1634/stemcells.2005-0612.
14. Gronthos, S., Mankani, M., Brahim, J., Robey, P. G., and Shi, S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc. Natl. Acad. Sci. U.S.A. 97, 13625-13630. doi: 10.1073/pnas.240309797.
15. Hall, B. K. (2008). The neural crest and neural crest cells: discovery and significance for theories of embryonic organization. J. Biosci. 33, 781-793. doi: 10.1007/s12038-008-0098-4.
16. Hirata, T. M., Ishkitiev, N., Yaegaki, K., Calenic, B., Ishikawa, H., Nakahara, T., et al. (2010). Expression of multiple stem cell markers in dental pulp cells cultured in serum-free media. J. Endod. 36, 1139-1144. doi: 10.1016/j.joen.2010.03.002.
17. Horwitz, E. M., Gordon, P. L., Koo, W. K., Marx, J. C., Neel, M. D., McNall, R. Y., et al. (2002). Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: implications for cell therapy of bone. Proc. Natl. Acad. Sci. U.S.A. 99, 8932-8937. doi: 10.1073/pnas.132252399.
18. Huang, A. H., Snyder, B. R., Cheng, P. H., and Chan, A. W. (2008). Putative dental pulp-derived stem/stromal cells promote proliferation and differentiation of endogenous neural cells in the hippocampus of mice. Stem Cells 26, 2654-2663. doi: 10.1634/stemcells2008-0285.
19. Hung, C. N., Mar, K., Chang, H. C., Chiang, Y. L., Hu, H. Y., Lai, C. C., et al. (2011). A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration. Biomaterials 32, 6995-7005. doi: 10.1016/j.biomaterials.2011.05.086.
20. Iohara, K., Zheng, L., Ito, M., Tomokiyo, A., Matsushita, K., and Nakashima, M. (2006). Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells 24, 2493-2503. doi: 10.1634/stemcells.2006-0161.
21. Ishizaka, R., Hayashi, Y., Iohara, K., Sugiyama, M., Murakami, M., Yamamoto, T., et al. (2013). Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. Biomaterials 34, 1888-1897. doi: 10.1016/j.biomaterials.2012.10.045.
22. Karbanova, J., Soukup, T., Suchanek, J., Pytlik, R., Corbeil, D., and Mokry, J. (2011). Characterization of dental pulp stem cells from impacted third molars cultured in low serum-containing medium. Cells Tissues Organs 193, 344-365. doi: 10.1159/000321160.
23. Kiraly, M., Kadar, K., Horvathy, D. B., Nardai, P., Racz, G. Z., Lacza, Z., et al. (2011). Integration of neuronally predifferentiated human dental pulp stem cells into rat brain in vivo. Neurochem. Int. 59, 371-381. doi: 10.1016/j.neuint.2011.01.006.
24. Kuznetsov, S. A., Mankani, M. H., and Robey, P. G. (2000). Effect of serum on human bone marrow stromal cells: ex vivo expansion and in vivo bone formation. Transplantation 70, 1780-1787. doi: 10.1097/00007890-200012270-00018.
25. Lacerda-Pinheiro, S., Dimitrova-Nakov, S., Harichane, Y., Souyri, M., Petit-Cocault, L., Legres, L., et al. (2012). Concomitant multipotent and unipotent dental pulp progenitors and their respective contribution to mineralised tissue formation. Eur. Cell. Mater. 23, 371-386.
26. Leong, W. K., Henshall, T. L., Arthur, A., Kremer, K. L., Lewis, M. D., Helps, S. C., et al. (2012). Human adult dental pulp stem cells enhance poststroke functional recovery through non-neural replacement mechanisms. Stem Cells Transl. Med. 1, 177-187. doi: 10.5966/sctm.2011-0039.
27. Livak, K. J., and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402-408. doi: 10.1006/meth.2001.1262.
28. Lopez-Cazaux, S., Bluteau, G., Magne, D., Lieubeau, B., Guicheux, J., and Alliot-Licht, B. (2006). Culture medium modulates the behaviour of human dental pulp-derived cells: technical note. Eur. Cell. Mater. 11, 35-42. discussion: 42.
29. Moos, T., and Morgan, E. H. (2000). Transferrin and transferrin receptor function in brain barrier systems. Cell. Mol. Neurobiol. 20, 77-95.
30. Moos, T., and Morgan, E. H. (2002). A morphological study of the developmentally regulated transport of iron into the brain. Dev. Neurosci. 24, 99-105. doi: 10.1159/000065702.
31. Paino, F., La Noce, M., Tirino, V., Naddeo, P., Desiderio, V., Pirozzi, G., et al. (2013). Histone Deacetylase inhibition with Valproic Acid down-regulates Osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement. Stem Cells. doi: 10.1002/stem.1544. [Epub ahead of print].
32. Richardson, W. D., Smith, H. K., Sun, T., Pringle, N. P., Hall, A., and Woodruff, R. (2000). Oligodendrocyte lineage and the motor neuron connection. Glia 29, 136-142. doi: 10.1002/(SICI)1098-1136(20000115)29:2<136::AID-GLIA6>3.0.CO;2-G.
33. Schiraldi, C., Stellavato, A., D'Agostino, A., Tirino, V., d'Aquino, R., Woloszyk, A., et al. (2012). Fighting for territories: time-lapse analysis of dental pulp and dental follicle stem cells in co-culture reveals specific migratory capabilities. Eur. Cell. Mater. 24, 426-440.
34. Sergent-Tanguy, S., Veziers, J., Bonnamain, V., Boudin, H., Neveu, I., and Naveilhan, P. (2006). Cell surface antigens on rat neural progenitors and characterization of the CD3 (+)/CD3 (-) cell populations. Differentiation 74, 530-541. doi: 10.1111/j.1432-0436.2006.00098.x.
35. Tirino, V., Paino, F., d'Aquino, R., Desiderio, V., De Rosa, A., and Papaccio, G. (2011). Methods for the identification, characterization and banking of human DPSCs: current strategies and perspectives. Stem Cell Rev. 7, 608-615. doi: 10.1007/s12015-011-9235-9.
36. Tran, P. B., Banisadr, G., Ren, D., Chenn, A., and Miller, R. J. (2007). Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brain. J. Comp. Neurol. 500, 1007-1033. doi: 10.1002/cne.21229.
37. Wessman, S. J., and Levings, R. L. (1999). Benefits and risks due to animal serum used in cell culture production. Dev. Biol. Stand. 99, 3-8.
38. Yamagata, M., Yamamoto, A., Kako, E., Kaneko, N., Matsubara, K., Sakai, K., et al. (2013). Human dental pulp-derived stem cells protect against hypoxic-ischemic brain injury in neonatal mice. Stroke 44, 551-554. doi: 10.1161/STROKEAHA.112.676759.