A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis

Journal of Biomolecular Techniques

Volumn 24, Issue 4, 2013, Pages 224-231

  Ghatak, Souvik ^a   Muthukumaran, Rajendra Bose ^a   Nachimuthu, Senthil Kumar ^a  

^a MIZORAM UNIVERSITY   (India)

Author keywords

Mitochondrial; Quick prep; Restriction enzyme

Indexed keywords

GENOMIC DNA;

ADULT; ARTICLE; DNA EXTRACTION; DNA ISOLATION; FORENSIC ANALYSIS; GEL ELECTROPHORESIS; GENETIC PURITY; HAIR ANALYSIS; HUMAN; HUMAN EXPERIMENT; NORMAL HUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPECTROPHOTOMETRY; URINALYSIS;

MITOCHONDRIAL; QUICK-PREP; RESTRICTION ENZYME;

ADULT; DNA; HAIR; HUMANS; MOUTH MUCOSA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; YOUNG ADULT;

EID: 84891553048     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: 10.7171/jbt.13-2404-001     Document Type: Article

References (20)

1. Le Marchand L, Lum-Jones A, Saltzman B, Visaya V, Nomura AM, Kolonel LN. Feasibility of collecting buccal cell DNA by mail in a cohort study. Cancer Epidemiol Biomarkers Prev 2001; 10:701-703.
2. Hansen TV, Simonsen MK, Nielsen FC, Hundrup YA. Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic DNA. Cancer Epidemiol Biomarkers Prev 2007;16:2072-2076.
3. Van-Wieren-de-Wijer DB, Maitland-van-der-Zee AH, de Boer A, et al. Determinants of DNA yield and purity collected with buccal cell samples. Eur J Epidemiol 2009;24:677-682.
4. Emi S, Hiroshi N. Evaluation of three methods for effective extraction of DNA from human hair. J Chromatog B 2005;820: 137-141.
5. Graffy EA, Foran DR. A simplified method for mitochondrial DNA extraction from head hair shafts. J Forensic Sci 2005;50: 119-1122.
6. Mulot C, Stücker I, Clavel J, Beaune P, Loriot MA. Collection of human genomic DNA from buccal cells for genetics studies: comparison between cytobrush, mouthwash, and treated card. J Biomed Biotechnol 2005;2005:291-296.
7. Wilson MR, Polanskey D, Butler J, DiZinno JA, Repogle J, Budowle B. Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts. BioTechniques 1995;18:662-669.
8. Wilson MR, DiZinno JA, Polanskey D, Repogle J, Budowle B. Validation of mitochondrial DNA sequencing for forensic casework analysis. Int J Legal Med 1995;108:68-74.
9. Bisbing RE. The forensic identification and association of human hair. In Saferstein R (ed): Forensic Science Handbook, vol 1. EnglewoodCliffs, NJ, USA: Prentice Hall Regents, 1982;184-221.
10. Yasuda T, Iida R, Takeshita H, et al. A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit. J Forensic Sci 2003;48:108-110.
11. Deelman LE, Esther A, Wouden VD, Duin M, Henning RH. A method for the ultra rapid isolation of PCR-ready DNA from urine and buccal swabs. Mol Biol Today 2002;3:51-54.
12. Salas A, Lareu MV, Carracedo A. Heteroplasmy in mtDNA and the weight of evidence in forensic mtDNA analysis: a case report. Int J Legal Med 2001;114:186-190.
13. Doosti A, Ghasemi-Dehkordi P. Genetic polymorphisms of mitochondrial genome D-loop region inBakhtiarian population by PCR-RFLP. Int J Biol 2011;3:41-46.
14. Tsung-Hsing C, Sheng-Pyng C, Tzu-Chuan L, Wen-Chi C, Jenn-Shing S, Yi-Shing S. Optimal DNA extraction from buccal swab samples. J Med Sci 2010;30:149-154.
15. Chan AK, Chiu RW, Lo YM, Clinical Sciences Reviews Committee of the Association of Clinical Biochemists. Cell-free nucleic acids in plasma, serum and urine: a new tool in molecular diagnosis. Ann Clin Biochem 2003;40:122-130.
16. Harty LC, Garcia-Closas M, Rothman N, Reid YA, Tucker MA, Hartge P. Collection of buccal cell DNA using treated cards. Cancer Epidemiol Biomarkers Prev 2000;9:501-506.
17. Freeman B, Powell J, Ball D, Hill L, Craig I, Plomin R. DNA by mail: an inexpensive and noninvasive method for collecting DNA samples from widely dispersed populations. Behav Genet May 1997;27:251-257.
18. King IB, Satia-Abouta J, Thornquist MD, et al. Buccal cell DNA yield, quality, and collection costs: comparison of methods for large scale studies. Cancer Epidemiol Biomarkers Prev 2002;11:1130-1133.
19. Russell H, Von-Beroldingen CH, Sensabaugh GF, Erlich HA. DNA typing from single hairs. Nature 1988;332:543-546.
20. Linch CA, Whiting DA, Holland MM. Human hair histogen-esis for the mitochondrial DNA forensic scientist. J Forensic Sci 2001;46:844-853.