Fabrication of photo-crosslinked Chitosan- Gelatin scaffold in sodium alginate hydrogel for chondrocyte culture

Bio-Medical Materials and Engineering

Volumn 24, Issue 1, 2014, Pages 633-641

  Zhao, Peng ^a   Deng, Cuijun ^a   Xu, Hongzhen ^b   Tang, Xing ^a   He, Hailong ^c   Lin, Chao ^a   Su, Jiansheng ^b  

^a TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

^b TONGJI UNIVERSITY   (China)

^c SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

cartilage tissue engineering; chitosan; chondrocyte; gelatin; hydrogel

Indexed keywords

CARTILAGE TISSUE ENGINEERING; CHITOSAN-GELATIN SCAFFOLDS; CHONDROCYTES; CROSS LINKING AGENTS; GELATIN; MICROSTRUCTURE AND MECHANICAL PROPERTIES; SCANNING ELECTRONIC MICROSCOPY (SEM); VISCOELASTIC MEASUREMENTS;

BIOCOMPATIBILITY; BIOMEDICAL ENGINEERING; BIOTECHNOLOGY; BODY FLUIDS; CARTILAGE; CHITOSAN; HYDROGELS; INTELLIGENT AGENTS; MECHANICAL PROPERTIES; SODIUM ALGINATE;

SCAFFOLDS (BIOLOGY);

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; ALGINIC ACID; CHITOSAN; GELATIN; MOLECULAR SCAFFOLD;

ANIMAL CELL; BIOCOMPATIBILITY; CARTILAGE CELL; CHEMICAL STRUCTURE; COMPRESSION; CONFERENCE PAPER; CROSS LINKING; HYDROGEL; IN VITRO STUDY; INFRARED SPECTROSCOPY; MECHANICS; NANOFABRICATION; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; SCANNING ELECTRON MICROSCOPY; SYNTHESIS; VISCOELASTICITY;

CARTILAGE TISSUE ENGINEERING; CHITOSAN; CHONDROCYTE; GELATIN; HYDROGEL;

ALGINATES; ANIMALS; BIOCOMPATIBLE MATERIALS; CALCIUM CHLORIDE; CARTILAGE; CELL PROLIFERATION; CELLS, CULTURED; CHITOSAN; CHONDROCYTES; CROSS-LINKING REAGENTS; ELASTICITY; GELATIN; GLUCURONIC ACID; HEXURONIC ACIDS; HYDROGELS; IMAGING, THREE-DIMENSIONAL; MAGNETIC RESONANCE SPECTROSCOPY; MICROSCOPY, ELECTRON, SCANNING; OXAZINES; PHENOTYPE; PHOTOCHEMISTRY; RABBITS; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TISSUE ENGINEERING; TISSUE SCAFFOLDS; VISCOSITY; XANTHENES;

EID: 84891122569     PISSN: 09592989     EISSN: None     Source Type: Journal    
DOI: 10.3233/BME-130851     Document Type: Conference Paper

References (28)

1. R. Cortesini, Stem cells, tissue engineering and organogenesis in transplantation, Transpl. Immunol. 15 (2005), 81-89.
2. N.R. Mercier, H.R. Costantino, M.A. Tracy and L.J. Bonassar, Poly(lactide-co-glycolide) microspheres as a moldable scaffold for cartilage tissue engineering, Biomaterials 26 (2005), 1945-1952.
3. H.S. Yoo, E.A. Lee, J.J. Yoon and T.G. Park, Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering, Biomaterials 26 (2005), 1925-1933.
4. C. Vinatier, C. Bouffi, C. Merceron, J. Gordeladze, J.M. Brondello, C. Jorgensen, P. Weiss, J. Guicheux and D. Noel, Cartilage Tissue Engineering: Towards a Biomaterial-Assisted Mesenchymal Stem Cell Therapy, Curr. Stem. Cell. Res. Ther. 4 (2009), 318-329.
5. J.M. Moran, D. Pazzano and L.J. Bonassar, Characterization of polylactic acid-polyglycolic acid composites for cartilage tissue engineering, Tissue Eng. 9 (2003), 63-70.
6. L.E. Freed, D.A. Grande, Z. Lingbin, J. Emmanual, J.C. Marquis and R. Langer, Joint Resurfacing Using Allograft Chondrocytes and Synthetic Biodegradable Polymer Scaffolds, J. Biomed. Mater. Res. 28 (1994), 891-899.
7. D. Schaefer, I. Martin, G. Jundt, J. Seidel, M. Heberer, A. Grodzinsky, I. Bergin, G. Vunjak-Novakovic and L.E. Freed, Tissue-engineered composites for the repair of large osteochondral defects, Arthritis Rheum. 46 (2002), 2524-2534.
8. T.J. Klein, S.C. Rizzi, K. Schrobback, J.C. Reichert, J.E. Jeon, R.W. Crawford and D.W. Hutmacher, Long-term effects of hydrogel properties on human chondrocyte behavior, Soft Matter 6 (2010), 5175-5183.
9. S. Nishimoto, M. Takagi, S. Wakitani, T. Nihira and T. Yoshida, Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes, J. Biosci. Bioeng. 100 (2005), 123-126.
10. L. Danisovic, I. Varga, R. Zamborsky and D. Bohmer, The tissue engineering of articular cartilage: Cells, scaffolds and stimulating factors, Exp. Biol. Med. 237 (2012), 10-17.
11. M.M. Stevens and J.H. George, Exploring and engineering the cell surface interface, Science 310 (2005), 1135-1138.
12. M.M. Stevens, Exploring and Engineering the Cell-Surface Interface, Biophys. J. 100 (2011), 189-189.
13. S.J. Bryant and K.S. Anseth, The effects of scaffold thickness on tissue engineered cartilage in photocrosslinked poly(ethylene oxide) hydrogels, Biomaterials 22 (2001), 619-626.
14. A. Di Martino, M. Sittinger and M.V. Risbud, Chitosan: A versatile biopolymer for orthopaedic tissue-engineering, Biomaterials 26 (2005), 5983-5990.
15. B.H. Tsai, C.H. Lin and J.C. Lin, Synthesis and property evaluations of photocrosslinkable chitosan derivative and its photocopolymerization with poly(ethylene glycol), J. Appl. Polym. Sci. 100 (2006), 1794-1801.
16. D.L. Nettles, S.H. Elder and J.A. Gilbert, Potential use of chitosan as a cell scaffold material for cartilage tissue engineering, Tissue Eng. 8 (2002), 1009-1016.
17. C.H. Chang, H.C. Liu, C.C. Lin, C.H. Chou and F.H. Lin, Gelatin-chondroitin-hyaluronan tri-copolymer scaffold for cartilage tissue engineering, Biomaterials 24 (2003), 4853-4858.
18. W.Y. Xia, W. Liu, L. Cui, Y.C. Liu, W. Zhong, D.L. Liu, J.J. Wu, K.H. Chua and Y.L. Cao, Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds, J. Biomed. Mater. Res. B. 71B (2004), 373-380.
19. H. Tan, Y. Gong, L. Lao, Z. Mao and C. Gao, Gelatin/chitosan/hyaluronan ternary complex scaffold containing basic fibroblast growth factor for cartilage tissue engineering, J. Mater. Sci. Mater. Med. 18 (2007), 1961-1968.
20. A.T. Mehlhorn, H. Schmal, S. Kaiser, G. Lepski, G. Finkenzeller, G.B. Stark and N.P. Sudkamp, Mesenchymal stem cells maintain TGF-beta-mediated chondrogenic phenotype in alginate bead culture, Tissue Eng. 12 (2006), 1393-1403.
21. Y. Qiu, N. Zhang, Q. Kang, Y. An and X. Wen, Chemically modified light-curable chitosans with enhanced potential for bone tissue repair, J. Biomed. Mater. Res. A 89 (2009), 772-779.
22. F. Li, Q.J. Ba, S.M. Niu, Y. Guo, Y.K. Duan, P. Zhao, C. Lin and J. Sun, In-situ forming biodegradable glycol chitosanbased hydrogels: Synthesis, characterization, and chondrocyte culture, Mat. Sci. Eng. C-Mater. 32 (2012), 2017-2025.
23. V.K. Mourya and N.N. Inamdar, Chitosan-modifications and applications: Opportunities galore, React. Funct. Polym. 68 (2008), 1013-1051.
24. V. Dodane and V.D. Vilivalam, Pharmaceutical applications of chitosan, Pharm. Sci. Technol. To. 1 (1998), 246-253.
25. E.J. Miller, Isolation and characterization of a collagen from chick cartilage containing three identical alpha chains, Biochemistry 10 (1971), 1652-1659.
26. M. Stephens, A.P. Kwan, M.T. Bayliss and C.W. Archer, Human articular surface chondrocytes initiate alkaline phosphatase and type X collagen synthesis in suspension culture, J. Cell. Sci. 103 ( Pt 4) (1992), 1111-1116.
27. K. Deshmukh and B.D. Sawyer, Synthesis of collagen by chondrocytes in suspension culture: Modulation by calcium, 3':5'-cyclic AMP, and prostaglandins, Proc. Natl. Acad. Sci. USA. 74 (1977), 3864-3868.
28. S.S. Ng, K. Su, C. Li, M.B. Chan-Park, D.A. Wang and V. Chan, Biomechanical study of the edge outgrowth phenomenon of encapsulated chondrocytic isogenous groups in the surface layer of hydrogel scaffolds for cartilage tissue engineering, Acta Biomater. 8 (2012), 244-252.