Critical assessment of glyco- and phospholipid separation by using silica chromatography

Applied and Environmental Microbiology

Volumn 80, Issue 1, 2014, Pages 360-365

  Heinzelmann, Sandra M ^a   Bale, Nicole J ^a   Hopmans, Ellen C ^a   Sinninghe Damsté, Jaap S ^a   Schouten, Stefan ^a   van der Meer, Marcel T J ^a  

^a ROYAL NETHERLANDS INSTITUTE FOR SEA RESEARCH   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CRITICAL ASSESSMENT; GLYCO- AND PHOSPHOLIPIDS; ISOLATED MICROORGANISMS; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; MICROBIAL COMMUNITIES; MODIFIED CONDITIONS; SEPARATION METHODS; SILICA COLUMN CHROMATOGRAPHY;

FATTY ACIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROORGANISMS; PHOSPHOLIPIDS;

CHROMATOGRAPHY;

ALCOHOL; ASSESSMENT METHOD; FATTY ACID; MICROBIAL COMMUNITY; PHOSPHOLIPID; PHYLOGENETICS; SILICA;

GLYCOLIPID; PHOSPHOLIPID; SILICON DIOXIDE;

ARTICLE; CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY;

CHROMATOGRAPHY; GLYCOLIPIDS; PHOSPHOLIPIDS; SILICON DIOXIDE;

EID: 84891066230     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02817-13     Document Type: Article

References (35)

1. Amann RI, Ludwig W, Schleifer KH. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169.
2. Bobbie RJ, White DC. 1980. Characterization of benthic microbial community structure by high-resolution gas-chromatography of fatty-acid methyl-esters. Appl. Environ. Microbiol. 39:1212-1222.
3. Lechevalier MP. 1977. Lipids in bacterial taxonomy-taxonomists view. Crit. Rev. Microbiol. 5:109-210. http://dx.doi.org/10.3109/10408417709102311.
4. Shaw N. 1974. Lipid composition as a guide to the classification of bacteria. Adv. Appl. Microbiol. 17:63-108. http://dx.doi.org/10.1016/S0065-2164(08)70555-0.
5. Harvey HR, Fallon RD, Patton JS. 1986. The effect of organic-matter and oxygen on the degradation of bacterial-membrane lipids in marine sediments. Geochim. Cosmochim. Acta 50:795-804. http://dx.doi.org/10.1016/0016-7037(86)90355-8.
6. White DC, Davis WM, Nickels JS, King JD, Bobbie RJ. 1979. Determination of the sedimentary microbial biomass by extractible lipid phosphate. Oecologia 40:51-62. http://dx.doi.org/10.1007/BF00388810.
7. Kates M. 1964. Bacterial lipids. Adv. Lipid Res. 2:17-90.
8. Hölzl G, Dörmann P. 2007. Structure and function of glycoglycerolipids in plants and bacteria. Prog. Lipid Res. 46:225-243. http://dx.doi.org/10.1016/j.plipres.2007.05.001.
9. Boschker HTS. 2004. Linking microbial community structure and functioning: stable isotope (13C) labeling in combination with PLFA analysis, p 1673-1688. In Kowalchuk GA, de Bruijn FJ, Head IM, Akkermans AD, van Elsas JD (ed), Molecular microbial ecology manual II. Kluwer Academic Publishers, Dodrecht, The Netherlands
10. Dijkman NA, Boschker HTS, Stal LJ, Kromkamp JC. 2010. Composition and heterogeneity of the microbial community in a coastal microbial mat as revealed by the analysis of pigments and phospholipid-derived fatty acids. J. Sea Res. 63:62-70. http://dx.doi.org/10.1016/j.seares.2009.10.002.
11. Rütters H, Sass H, Cypionka H, Rullkotter J. 2002. Phospholipid analysis as a tool to study complex microbial communities in marine sediments. J. Microbiol. Methods 48:149-160. http://dx.doi.org/10.1016/S0167-7012(01)00319-0.
12. Boschker HTS, Middelburg JJ. 2002. Stable isotopes and biomarkers in microbial ecology. FEMS Microbiol. Methods 40:85-95. http://dx.doi.org/10.1111/j.1574-6941.2002.tb00940.x.
13. Boschker HTS, Nold SC, Wellsbury P, Bos D, De Graaf W, Pel R, Parkes RJ, Cappenberg TE. 1998. Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers. Nature 392:801-805. http://dx.doi.org/10.1038/33900.
14. Bodelier PL, Gillisen MJ, Hordijk K, Sinninghe Damsté JS, Rijpstra WI, Geenevasen JA, Dunfield PF. 2009. A reanalysis of phospholipid fatty acids as ecological biomarkers for methanotrophic bacteria. ISME J. 3:606-617. http://dx.doi.org/10.1038/ismej.2009.6.
15. Vorbeck ML, Marinetti GV. 1965. Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography. J. Lipid Res. 6:3-6.
16. Radin NS, Lavin FB, Brown JR. 1955. Determination of cerebrosides. J. Biol. Chem. 217:789-796.
17. Harris WD, Popat P. 1954. Determination of the phosphorus content of lipids. J. Am. Oil Chem. Soc. 31:124-127. http://dx.doi.org/10.1007/BF02545694.
18. Marinetti GV, Erbland J, Albrecht M, Stotz E. 1957. The application of chromatographic methods to study the incorporation of 32P-labeled orthophosphate into the phosphatides of rat liver homogenates. Biochim. Biophys. Acta 25:585-591. http://dx.doi.org/10.1016/0006-3002(57)90531-0.
19. Gehron M, White DC. 1983. Sensitive assay of phospholipid glycerol in environmental samples. J. Microbiol. Methods 1:23-32. http://dx.doi.org/10.1016/0167-7012(83)90004-0.
20. Guckert JB, Antworth CP, Nichols PD, White DC. 1985. Phospholipid, ester-linked fatty-acid profiles as reproducible assays for changes in prokaryotic community structure of estuarine sediments. FEMS Microbiol. Methods 31:147-158. http://dx.doi.org/10.1016/0378-1097(85)90016-3.
21. King JD, White DC, Taylor CW. 1977. Use of lipid-compostion and metabolism to examine structure and activity of estuarine detrital microflora. Appl. Environ. Microbiol. 33:1177-1183.
22. Rajendran N, Matsuda O, Imamura N, Urushigawa Y. 1992. Variation in microbial biomass and community structure in sediments of eutrophic bays as determined by phospholipid ester-linked fatty-acids. Appl. Environ. Microbiol. 58:562-571.
23. White DC, Frerman FE. 1967. Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus. J. Bacteriol. 94:1854-1867.
24. Zelles L. 1999. Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review. Biol. Fertil. Soils 29:111-129. http://dx.doi.org/10.1007/s003740050533.
25. Brandsma J, Hopmans EC, Brussaard CPD, Witte HJ, Schouten S, Sinninghe Damsté JS. 2012. Spatial distribution of intact polar lipids in North Sea surface waters: relationship with environmental conditions and microbial community composition. Limnol. Oceanogr. 57:959-973. http://dx.doi.org/10.4319/lo.2012.57.4.0959.
26. Brandsma J, Hopmans EC, Philippart CJM, Veldhuis MJW, Schouten S, Sinninghe Damsté JS. 2012. Low temporal variation in the intact polar lipid composition of North Sea coastal marine water reveals limited chemotaxonomic value. Biogeosciences 9:1073-1084. http://dx.doi.org/10.5194/bg-9-1073-2012.
27. Van Mooy BAS, Fredricks HF. 2010. Bacterial and eukaryotic intact polar lipids in the eastern subtropical South Pacific: water-column distribution, planktonic sources, and fatty acid composition. Geochim. Cosmochim. Acta 74:6499-6516. http://dx.doi.org/10.1016/j.gca.2010.08.026.
28. Van Mooy BAS, Fredricks HF, Pedler BE, Dyhrman ST, Karl DM, Koblizek M, Lomas MW, Mincer TJ, Moore LR, Moutin T, Rappé MS, Webb EA. 2009. Phytoplankton in the ocean use non-phosphorus lipids in response to phosphorus scarcity. Nature 458:69-72. http://dx.doi.org/10.1038/nature07659.
29. Van Mooy BAS, Rocap G, Fredricks HF, Evans CT, Devol AH. 2006. Sulfolipids dramatically decrease phosphorus demand by picocyanobacteria in oligotrophic marine environments. Proc. Natl. Acad. Sci. U. S. A. 103:8607-8612. http://dx.doi.org/10.1073/pnas.0600540103.
30. Sturt HF, Summons RE, Smith K, Elvert M, Hinrichs K-U. 2004. Intact polar membrane lipids in prokaryotes and sediments deciphered by highperformance liquid chromatography/electrospray ionization multistage mass spectrometry-new biomarkers for biogeochemistry and microbial ecology. Rapid Commun. Mass Spectrom. 18:617-628. http://dx.doi.org/10.1002/rcm.1378.
31. Zink KG, Wilkes H, Disko U, Elvert M, Horsfield B. 2003. Intact phospholipids-microbial "life markers" in marine deep subsurface sediments. Org. Geochem. 34:755-769. http://dx.doi.org/10.1016/S0146-6380(03)00041-X.
32. Bolhuis H, Stal LJ. 2011. Analysis of bacterial and archaeal diversity in coastal microbial mats using massive parallel 16S rRNA gene tag sequencing. ISMEJ 5:1701-1712. http://dx.doi.org/10.1038/ismej.2011.52.
33. Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917. http://dx.doi.org/10.1139/o59-099.
34. Brügger B, Erben G, Sandhoff R, Wieland FT, Lehmann WD. 1997. Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 94:2339-2344. http://dx.doi.org/10.1073/pnas.94.6.2339.
35. Schubotz F, Wakeham SG, Lipp JS, Fredricks HF, Hinrichs K-U. 2009. Detection of microbial biomass by intact polar membrane lipid analysis in the water column and surface sediments of the Black Sea. Environ. Microbiol. 11:2720-2734. http://dx.doi.org/10.1111/j.1462-2920.2009.01999.x.