Improving d-glucaric acid production from myo-inositol in E. coli by increasing MIOX stability and myo-inositol transport

Metabolic Engineering

Volumn 22, Issue , 2014, Pages 22-31

  Shiue, Eric ^a   Prather, Kristala L J ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

D glucaric acid; Directed evolution; Metabolic engineering; Myo inositol; Soluble protein fusions; Substrate transport

Indexed keywords

ACID PRODUCTION; DIRECTED EVOLUTION; MYO-INOSITOL; MYO-INOSITOL OXYGENASE; PROTEIN FUSION; RECOMBINANT E. COLI; SOLUBLE PROTEINS; SUBSTRATE TRANSPORTS;

ESCHERICHIA COLI; METABOLIC ENGINEERING; PROTEINS;

POLYOLS;

BACTERIUM LYSATE; DNA FRAGMENT; GLUTATHIONE TRANSFERASE; INOSITOL; INOSITOL OXYGENASE; MALTOSE BINDING PROTEIN; SACCHARIC ACID; HYBRID PROTEIN;

ALLOSTERISM; ARTICLE; BACTERIAL METABOLISM; CARBON SOURCE; COMPARATIVE STUDY; CONTROLLED STUDY; ENTAMOEBA COLI; ENZYME ACTIVITY; ENZYME STABILITY; EVOLUTION; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; SOLUBILITY; ACTIVE TRANSPORT; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM;

BIOLOGICAL TRANSPORT, ACTIVE; ENZYME STABILITY; ESCHERICHIA COLI; GLUCARIC ACID; INOSITOL; INOSITOL OXYGENASE; RECOMBINANT FUSION PROTEINS;

EID: 84891045972     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.12.002     Document Type: Article

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