Comparison of serum exosome isolation methods for microRNA profiling

Clinical Biochemistry

Volumn 47, Issue 1-2, 2014, Pages 135-138

  Rekker, Kadri ^a,b   Saare, Merli ^a,b   Roost, Anne Mari ^a   Kubo, Anna Liisa ^c   Zarovni, Natasa ^c   Chiesi, Antonio ^c   Salumets, Andres ^a,b   Peters, Maire ^a,b  

^a Competence Centre on Reproductive Medicine and Biology ^*  (Estonia)

^b UNIVERSITY OF TARTU   (Estonia)

^c HansaBioMed Ltd   (Estonia)

Author keywords

ExoQuick; Exosomes; Extracellular vesicles; MiRNA; Ultracentrifugation

Indexed keywords

MICRORNA;

ADULT; ARTICLE; ASSAY; CELL ISOLATION; CLINICAL ARTICLE; COMPARATIVE STUDY; EXOSOME; FEMALE; HUMAN; HUMAN CELL; PRECIPITATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SERUM; ULTRACENTRIFUGATION;

EQ; EXOQUICK; EXOQUICK PRECIPITATION SOLUTION; EXOSOMES; EXTRACELLULAR VESICLES; MIRNA; UC; ULTRACENTRIFUGATION;

EXOSOMES; HUMANS; MICRORNAS; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE VALUES; ULTRACENTRIFUGATION;

EID: 84890562207     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2013.10.020     Document Type: Article

References (14)

1. Mathivanan S., Ji H., Simpson R.J. Exosomes: extracellular organelles important in intercellular communication. J Proteomics 2010, 73:1907-1920.
2. Raposo G., Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 2013, 200:373-383.
3. Valadi H., Ekstrom K., Bossios A., Sjostrand M., Lee J.J., Lotvall J.O. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 2007, 9:654-659.
4. Taylor D.D., Gercel-Taylor C. MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol 2008, 110:13-21.
5. Tanaka Y., Kamohara H., Kinoshita K., Kurashige J., Ishimoto T., Iwatsuki M., et al. Clinical impact of serum exosomal microRNA-21 as a clinical biomarker in human esophageal squamous cell carcinoma. Cancer 2013, 119:1159-1167.
6. Liang B., Peng P., Chen S., Li L., Zhang M., Cao D., et al. Characterization and proteomic analysis of ovarian cancer-derived exosomes. J Proteomics 2013, 80C:171-182.
7. Murakami Y., Toyoda H., Tanahashi T., Tanaka J., Kumada T., Yoshioka Y., et al. Comprehensive miRNA expression analysis in peripheral blood can diagnose liver disease. PLoS One 2012, 7:e48366.
8. Merchant M.L., Powell D.W., Wilkey D.W., Cummins T.D., Deegens J.K., Rood I.M., et al. Microfiltration isolation of human urinary exosomes for characterization by MS. Proteomics Clin Appl 2010, 4:84-96.
9. Alvarez M.L., Khosroheidari M., Kanchi Ravi R., DiStefano J.K. Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int 2012, 82:1024-1032.
10. Tauro B.J., Greening D.W., Mathias R.A., Ji H., Mathivanan S., Scott A.M., et al. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 2012, 56:293-304.
11. Gyorgy B., Modos K., Pallinger E., Paloczi K., Pasztoi M., Misjak P., et al. Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood 2011, 117:e39-e48.
12. Taylor D.D., Zacharias W., Gercel-Taylor C. Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol 2011, 728:235-246.
13. Momen-Heravi F., Balaj L., Alian S., Trachtenberg A.J., Hochberg F.H., Skog J., et al. Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles. Front Physiol 2012, 3:162.
14. Arroyo J.D., Chevillet J.R., Kroh E.M., Ruf I.K., Pritchard C.C., Gibson D.F., et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A 2011, 108:5003-5008.