Knockdown of p62/sequestosome 1 attenuates autophagy and inhibits colorectal cancer cell growth

Molecular and Cellular Biochemistry

Volumn 385, Issue 1-2, 2014, Pages 95-102

  Zhou, Jianping ^a   Ren, Feng ^a   Shu, Guoshun ^a   Liu, Ganglei ^a   Liu, Dongcai ^a   Zhou, Jiapeng ^a   Yuan, Lianwen ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Autophagy; Colorectal cancer; Knockdown; p62 sequestosome 1

Indexed keywords

PROTEIN P62; SEQUESTOSOME 1; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ADULT; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTOPHAGY; CANCER INHIBITION; COLON CARCINOGENESIS; COLORECTAL CANCER; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FEMALE; GENE SILENCING; HUMAN; HUMAN CELL; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; TUMOR XENOGRAFT; WESTERN BLOTTING;

ANIMALS; AUTOPHAGY; CELL LINE, TUMOR; CELL PROLIFERATION; COLORECTAL NEOPLASMS; DOWN-REGULATION; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE KNOCKDOWN TECHNIQUES; HUMANS; MALE; MICE; MICROTUBULE-ASSOCIATED PROTEINS; MIDDLE AGED; RNA-BINDING PROTEINS; UP-REGULATION;

EID: 84890533190     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-013-1818-0     Document Type: Article

References (26)

1. Johansen T, Lamark T (2011) Selective autophagy mediated by autophagic adapter proteins. Autophagy 7(3):279-296
2. Moscat J, Diaz-Meco MT (2009) p62 at the crossroads of autophagy, apoptosis, and cancer. Cell 137(6):1001-1004. doi: 10.1016/j.cell.2009.05.023
3. Puissant A, Fenouille N, Auberger P (2012) When autophagy meets cancer through p62/SQSTM1. Am J Cancer Res 2(4):397-413
4. Mathew R, Karp CM, Beaudoin B, Vuong N, Chen G, Chen HY, Bray K, Reddy A, Bhanot G, Gelinas C, Dipaola RS, Karantza-Wadsworth V, White E (2009) Autophagy suppresses tumorigenesis through elimination of p62. Cell 137(6):1062-1075. doi: 10.1016/j.cell.2009.03.048
5. Stumptner C, Heid H, Fuchsbichler A, Hauser H, Mischinger HJ, Zatloukal K, Denk H (1999) Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent. Am J Pathol 154(6):1701-1710. doi: 10.1016/S0002-9440(10)65426-0
6. Thompson HG, Harris JW, Wold BJ, Lin F, Brody JP (2003) p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells. Oncogene 22(15):2322-2333. doi: 10.1038/sj.onc.1206325
7. Su Y, Qian H, Zhang J, Wang S, Shi P, Peng X (2005) The diversity expression of p62 in digestive system cancers. Clin Immunol 116(2):118-123. doi: 10.1016/j.clim.2005.04.004
8. Kitamura H, Torigoe T, Asanuma H, Hisasue SI, Suzuki K, Tsukamoto T, Satoh M, Sato N (2006) Cytosolic overexpression of p62 sequestosome 1 in neoplastic prostate tissue. Histopathology 48(2):157-161. doi: 10.1111/j.1365-2559.2005.02313.x
9. Inoue D, Suzuki T, Mitsuishi Y, Miki Y, Suzuki S, Sugawara S, Watanabe M, Sakurada A, Endo C, Uruno A, Sasano H, Nakagawa T, Satoh K, Tanaka N, Kubo H, Motohashi H, Yamamoto M (2012) Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma. Cancer Sci 103(4):760-766. doi: 10.1111/j.1349-7006.2012.02216.x
10. Bampton ET, Goemans CG, Niranjan D, Mizushima N, Tolkovsky AM (2005) The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes. Autophagy 1(1):23-36
11. Mizushima N, Yoshimori T, Levine B (2010) Methods in mammalian autophagy research. Cell 140(3):313-326. doi: 10.1016/j.cell.2010.01.028
12. Komatsu M (2011) Potential role of p62 in tumor development. Autophagy 7(9):1088-1090
13. Moscat J, Diaz-Meco MT (2012) p62: a versatile multitasker takes on cancer. Trends Biochem Sci 37(6):230-236. doi: 10.1016/j.tibs.2012.02.008
14. Nezis IP, Stenmark H (2012) p62 at the interface of autophagy, oxidative stress signaling, and cancer. Antioxid Redox Signal 17(5):786-793. doi: 10.1089/ars.2011.4394
15. Rolland P, Madjd Z, Durrant L, Ellis IO, Layfield R, Spendlove I (2007) The ubiquitin-binding protein p62 is expressed in breast cancers showing features of aggressive disease. Endocr Relat Cancer 14(1):73-80. doi: 10.1677/erc.1.01312
16. Inami Y, Waguri S, Sakamoto A, Kouno T, Nakada K, Hino O, Watanabe S, Ando J, Iwadate M, Yamamoto M, Lee MS, Tanaka K, Komatsu M (2011) Persistent activation of Nrf2 through p62 in hepatocellular carcinoma cells. J Cell Biol 193(2):275-284. doi: 10.1083/jcb.201102031jcb.201102031
17. Myung Park J, Huang S, Wu TT, Foster NR, Sinicrope FA (2012) Prognostic impact of Beclin 1, p62/sequestosome 1 and LC3 protein expression in colon carcinomas from patients receiving 5-fluorouracil as adjuvant chemotherapy. Cancer Biol Ther 14(2):100-107
18. Duran A, Amanchy R, Linares JF, Joshi J, Abu-Baker S, Porollo A, Hansen M, Moscat J, Diaz-Meco MT (2011) p62 is a key regulator of nutrient sensing in the mTORC1 pathway. Mol Cell 44(1):134-146. doi: 10.1016/j.molcel.2011.06.038
19. Pankiv S, Clausen TH, Lamark T, Brech A, Bruun JA, Outzen H, Overvatn A, Bjorkoy G, Johansen T (2007) p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem 282(33):24131-24145. doi: 10.1074/jbc.M702824200
20. Komatsu M, Waguri S, Koike M, Sou YS, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka K (2007) Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131(6):1149-1163. doi: 10.1016/j.cell.2007.10.035
21. Zheng Q, Su H, Ranek MJ, Wang X (2011) Autophagy and p62 in cardiac proteinopathy. Circ Res 109(3):296-308. doi: 10.1161/CIRCRESAHA.111. 244707CIRCRESAHA.111.244707
22. Zatloukal K, Stumptner C, Fuchsbichler A, Heid H, Schnoelzer M, Kenner L, Kleinert R, Prinz M, Aguzzi A, Denk H (2002) p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases. Am J Pathol 160(1):255-263. doi: 10.1016/S0002-9440(10)64369-6
23. Kirkin V, McEwan DG, Novak I, Dikic I (2009) A role for ubiquitin in selective autophagy. Mol Cell 34(3):259-269. doi: 10.1016/j.molcel.2009.04.026
24. Viiri J, Hyttinen JM, Ryhanen T, Rilla K, Paimela T, Kuusisto E, Siitonen A, Urtti A, Salminen A, Kaarniranta K (2010) p62/sequestosome 1 as a regulator of proteasome inhibitor-induced autophagy in human retinal pigment epithelial cells. Mol Vis 16:1399-1414. doi: 154
25. Parkhitko A, Myachina F, Morrison TA, Hindi KM, Auricchio N, Karbowniczek M, Wu JJ, Finkel T, Kwiatkowski DJ, Yu JJ, Henske EP (2011) Tumorigenesis in tuberous sclerosis complex is autophagy and p62/sequestosome 1 (SQSTM1)-dependent. Proc Natl Acad Sci USA 108(30):12455-12460. doi: 10.1073/pnas.1104361108
26. Takamura A, Komatsu M, Hara T, Sakamoto A, Kishi C, Waguri S, Eishi Y, Hino O, Tanaka K, Mizushima N (2011) Autophagy-deficient mice develop multiple liver tumors. Genes Dev 25(8):795-800. doi: 10.1101/gad.2016211