Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390)

BMC Plant Biology

Volumn 13, Issue 1, 2013, Pages

  Egert, Aurélie ^a,c   Keller, Felix ^a   Peters, Shaun ^b  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b STELLENBOSCH UNIVERSITY   (South Africa)

^c UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Abiotic stress; Arabidopsis; Galactinol; Raffinose; Raffinose synthase; Seeds; Water soluble carbohydrates

Indexed keywords

ARABIDOPSIS; ESCHERICHIA COLI;

ARABIDOPSIS PROTEIN; GALACTOSYLTRANSFERASE; RAFFINOSE SYNTHASE;

ARABIDOPSIS; ARTICLE; COLD; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PLANT LEAF; TRANSGENIC PLANT;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; COLD TEMPERATURE; GALACTOSYLTRANSFERASES; GENE EXPRESSION REGULATION, PLANT; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED;

EID: 84890495819     PISSN: None     EISSN: 14712229     Source Type: Journal    
DOI: 10.1186/1471-2229-13-218     Document Type: Article

References (23)

1. Bachmann M, Keller F. Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. (inter- and intracellular compartmentation). Plant Physiol 1995, 109:991-998. 161401, 12228647.
2. Haab CI, Keller F. Purification and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosyltransferase (GGT), from Ajuga reptans leaves. Physiologia Plant 2002, 114:361-371.
3. Tapernoux-Lüthi EM, Böhm A, Keller F. Cloning, functional expression, and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosyltransferase, from common bugle leaves. Plant Physiol 2004, 134:1377-1387. 10.1104/pp.103.036210, 419815, 15034167.
4. Peters S, Keller F. Frost tolerance in excised leaves of the common bugle (Ajuga reptans L.) correlates positively with the concentrations of raffinose family oligosaccharides (RFOs). Plant Cell Environ 2009, 32:1099-1107. 10.1111/j.1365-3040.2009.01991.x, 19422612.
5. Gilbert GA, Wilson C, Madore MA. Root-zone salinity alters raffinose oligosaccharide metabolism and transport in Coleus. Plant Physiol 1997, 115:1267-1276. 158591, 12223871.
6. Dierking EC, Bilyeu KD. Association of a soybean raffinose synthase gene with low raffinose and stachyose seed phenotype. Plant Gen 2008, 1:135-145.
7. Peterbauer T, Lahuta LB, Blöchl A, Mucha J, Jones DA, Hedley CL, Gòrecki RJ, Richter A. Analysis of the raffinose family oligosaccharide pathway in pea seeds with contrasting carbohydrate composition. Plant Physiol 2001, 127:1764-1772. 10.1104/pp.010534, 133579, 11743119.
8. Sui XL, Meng FZ, Wang HY, Wei YX, Li RF, Wang ZY, Hu LP, Wang SH, Zhang ZX. Molecular cloning, characteristics and low temperature response of raffinose synthase gene in Cucumis sativus L. J Plant Physiol 2012, 169:1883-1891. 10.1016/j.jplph.2012.07.019, 22985990.
9. Li S, Li T, Kim W-D, Kitaoka M, Yoshida S, Nakajima M, Kobayashi H. Characterization of raffinose synthase from rice (Oryza sativa L. var. Nipponbare). Biotech Letters 2007, 29:635-640.
10. Nishizawa A, Yabuta Y, Shigeoka S. Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Plant Physiol 2008, 147:1251-1263. 10.1104/pp.108.122465, 2442551, 18502973.
11. Maruyama K, Takeda M, Kidokoro S, Yamada K, Sakuma Y, Urano K, Fujita M, Yoshiwara K, Matsukura S, Morishita Y, et al. Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated by DREB1A and DREB2A. Plant Physiol 2009, 150:1972-1980. 10.1104/pp.109.135327, 2719109, 19502356.
12. Wu X, Kishitani S, Ito Y, Toriyama K. Accumulation of raffinose in rice seedlings overexpressing OsWRKY11 in relation to desiccation tolerance. Plant Biotech 2009, 26:431-434.
13. Peters S, Egert A, Stieger B, Keller F. Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern. Plant Cell Physiol 2010, 51:1815-1819. 10.1093/pcp/pcq127, 20739305.
14. Zuther E, Buchel K, Hundertmark M, Stitt M, Hincha DK, Heyer AG. The role of raffinose in the cold acclimation response of Arabidopsis thaliana. FEBS Lett 2004, 576:169-173. 10.1016/j.febslet.2004.09.006, 15474032.
15. Taji T, Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K. Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant J 2002, 29:417-426. 10.1046/j.0960-7412.2001.01227.x, 11846875.
16. Ooms J, Leon-Kloosterziel KM, Bartels D, Koornneef M, Karssen CM. Acquisition of desiccation tolerance and longevity in seeds of Arabidopsis thaliana (a comparative study using abscisic acid-insensitive abi3 mutants). Plant Physiol 1993, 102:1185-1191. 158904, 12231895.
17. Bentsink L, Alonso-Blanco C, Vreugdenhil D, Tesnier K, Groot SPC, Koornneef M. Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis. Plant Physiol 2000, 124:1595-1604. 10.1104/pp.124.4.1595, 59858, 11115877.
18. Nishizawa-Yokoi A, Yabuta Y, Shigeoka S. The contribution of carbohydrates including raffinose family oligosaccharides and sugar alcohols to protection of plant cells from oxidative damage. Plant Signal Behavior 2008, 3:1016-1018.
19. Oono Y, Seki M, Satou M, Iida K, Akiyama K, Sakurai T, Fujita M, Yamaguchi-Shinozaki K, Shinozaki K. Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays. Funct Integr Genomics 2006, 6:212-234. 10.1007/s10142-005-0014-z, 16463051.
20. Kempa S, Krasensky J, Dal Santo S, Kopka J, Jonak C. A central role of abscisic acid in stress-regulated carbohydrate metabolism. PLoS ONE 2008, 3:e3935. 10.1371/journal.pone.0003935, 2593778, 19081841.
21. Panikulangara TJ, Eggers-Schumacher G, Wunderlich M, Stransky H, Schöffl F. Galactinol synthase1. A novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis. Plant Physiol 2004, 136:3148-3158. 10.1104/pp.104.042606, 523375, 15466240.
22. Peters S, Mundree SG, Thomson JA, Farrant JM, Keller F. Protection mechanisms in the resurrection plant Xerophyta viscosa (Baker): both sucrose and raffinose family oligosaccharides (RFOs) accumulate in leaves in response to water deficit. J Exp Bot 2007, 58:1947-1956. 10.1093/jxb/erm056, 17452754.
23. Egert A, Peters S, Guyot C, Stieger B, Keller F. An Arabidopsis T-DNA insertion mutant for galactokinase (AtGALK, At3g06580) hyperaccumulates free galactose and is insensitive to exogenous galactose. Plant Cell Physiol 2012, 53:921-929. 10.1093/pcp/pcs036, 22437845.