Analysis of skeletal muscle defects in larval zebrafish by birefringence and touch-evoke escape response assays

Journal of Visualized Experiments

Volumn , Issue 82, 2013, Pages

  Smith, Laura L ^a   Beggs, Alan H ^a   Gupta, Vandana A ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Birefringence; Danio rerio; Dystrophy; Issue 82; Microscopy; Myopathy; Physiology; Touch evoked escape; Zebrafish

Indexed keywords

ANIMAL; ARTICLE; BIREFRINGENCE; CHEMISTRY; CONGENITAL MALFORMATION; DISEASE MODEL; ESCAPE BEHAVIOR; FEMALE; LARVA; MALE; MUSCULAR DYSTROPHY; PATHOLOGY; PATHOPHYSIOLOGY; PHYSIOLOGY; SKELETAL MUSCLE; SWIMMING; ZEBRA FISH;

ANIMALS; BIREFRINGENCE; DISEASE MODELS, ANIMAL; ESCAPE REACTION; FEMALE; LARVA; MALE; MUSCLE, SKELETAL; MUSCULAR DYSTROPHIES; SWIMMING; ZEBRAFISH;

EID: 84890265249     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/50925     Document Type: Article

References (18)

1. Bassett, D.I., Bryson-Richardson, R.J., Daggett, D.F., Gautier, P., Keenan, D.G., Currie, P.D. Dystrophin is required for the formation of stable muscle attachments in the zebrafish embryo. Development. 130, 5851-5860, 10.1242/dev.00799 (2003).
2. Gupta, V., et al. The zebrafish dag1 mutant: a novel genetic model for dystroglycanopathies. Hum. Mol. Genet. 20 (9), 1712-1725, 10.1093/hmg/ddr047 (2011).
3. Hall, T.E., et al. The zebrafish candyfloss mutant implicates extracellular matrix adhesion failure in laminin α2-deficient congenital muscular dystrophy. Proc. Natl. Acad. Sci. U.S.A. 104 (17), 7092-7097, 10.1073/pnas.0700942104 (2007).
4. Dowling, J.J., et al. Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathy. PLoS Genet. 5 (2), e1000372, 10.1371/journal. pgen.1000372 (2009).
5. Telfer, W.R., Nelson, D.D., Waugh, T., Brooks, S.V., Dowling, J.J. Neb: a zebrafish model of nemaline myopathy due to nebulin mutation. Dis. Model Mech. 5 (3), 389-396, 10.1242/dmm.008631 (2012).
6. Ferrante, M.I., Kiff, R.M., Goulding, D.A., Stemple, D.L. Troponin T is essential for sarcomere assembly in zebrafish skeletal muscle. J. Cell Sci. 124 (4), 565-577, 10.1242/jcs.071274 (2011).
7. Hawkins, T.A., et al. The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis. Development. 135 (6), 1147-1156, 10.1242/dev.018150 (2008).
8. Barbazuk, W.B., et al. The syntenic relationship of the zebrafish and human genomes. Genome Res. 10(9), 1351-1358, 10.1101/gr.144700 (2000).
9. Schapira, G., Dreyfus, J.C., Joly, M. Changes in the flow birefringence of myosin as a result of muscular atrophy. Nature. 170 (4325), 494-495, 10.1038/170494b0 (1952).
10. Kimmel, C.B., Patterson, J., Kimmel, R.O. The development and behavioral characteristics of the startle response in zebrafish. Dev. Psychobiol. 7, 47-60 (1974).
11. Eaton, R.C., Bombardieri, R.A., Meyer, D.L. The Mauthner-initiated startle response in teleost fish. J. Exp. Biol. 66, 65-81 (1977).
12. Saint-Amant, L., Drapeau, P. Time course of the development of motor behaviors in the zebrafish embryo.J. Neurobiol. 37 (4), 622-632, 10.1002/(SICI)1097-4695(199812)37:4<622::AID-NEU10>3.0. CO;2-S (1998).
13. Hirata, H., et al. Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development. 134 (15), 2771-2781, 10.1242/dev.004531 (2007).
14. Wallace, G.Q., McNally, E.M. Mechanisms of muscle degeneration, regeneration, and repair in the muscular dystrophies. Annu. Rev. Physiol. 71, 37-57, 10.1146/annurev. physiol.010908.163216 (2009).
15. Granato, M., et al. Genes controlling and mediating locomotion behavior of the zebrafish embryo and larva. Development. 123, 399-413 (1996).
16. Berger, J., Sztal, T., Currie, P.D. Quantification of birefringence readily measures the level of muscle damage in zebrafish. Biochem. Biophys. Res. Commun. 423 (4), 785-788, 10.1016/j. bbrc.2012.06.040 (2012).
17. Low, S.E., et al. TRPM7 is required within zebrafish sensory neurons for the activation of touch-evoked escape behaviors. J. Neurosci. 31 (32): 11633-11644, http://dx.doi.org/10.1523/JNEUROSCI.4950-10.2011 (2011).
18. Liu, D.W., Westerfield, M. Function of identified motoneurones and co-ordination of primary and secondary motor systems during zebrafish swimming. J. Physiol. 403, 73-89 (1988).