Isolation of lymphocytes from mouse genital tract mucosa

Journal of Visualized Experiments

Volumn , Issue 67, 2012, Pages

  Jiang, Janina ^a   Kelly, Kathleen A ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CALIFORNIA NANOSYSTEMS INSTITUTE   (United States)

Author keywords

FACS; Flow cytometry; Genital tract lymphocytes; Immunology; Issue 67; Lymphocyte isolation; Mucosal immunity; Sexually transmitted diseases

Indexed keywords

EID: 84889920315     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4391     Document Type: Article

References (11)

1. Renz, H., Brandtzaeg, P., & Hornef M. The impact of perinatal immune development on mucosal homeostasis and chronic inflammation. Nat. Rev Immunol. 12, 9-23 (2011).
2. 2010 Sexually Transmitted Diseases Surveillance. Centers for Disease Control and Prevention (2012).
3. Jain, S., Patrick, A.J., & Rosenthal, K.L. Multiple tandem copies of conserved gp41 epitopes incorporated in gag virus-like particles elicit systemic and mucosal antibodies in an optimized heterologous vector delivery regimen. Vaccine. 28, 7070-7080 (2010).
4. Tang, V.A. & Rosenthal, K.L. Intravaginal infection with herpes simplex virus type-2 (HSV-2) generates a functional effector memory T cell population that persists in the murine genital tract. J. Reprod. Immunol. 87, 39-44 (2010).
5. Gillgrass, A.E., Tang, V.A., Towarnicki, K.M., Rosenthal, K.L., & Kaushic, C. Protection against genital herpes infection in mice immunized under different hormonal conditions correlates with induction of vagina-associated lymphoid tissue. J. Virol. 79, 3117-3126 (2005).
6. Sajic, D., Patrick, A.J., & Rosenthal, K.L. Mucosal delivery of CpG oligodeoxynucleotides expands functional dendritic cells and macrophages in the vagina. Immunology. 114, 213-24 (2005).
7. Jiang, J.Q., Patrick, A., Moss, R.B., & Rosenthal, K.L. CD8+ T-cell-mediated cross-clade protection in the genital tract following intranasal immunization with inactivated human immunodeficiency virus antigen plus CpG oligodeoxynucleotides. J. Virol. 79,393-400 (2005).
8. Gill, N., Chenoweth, M.J., Verdu, E.F., & Ashkar, A.A. NK cells require type I IFN receptor for antiviral responses during genital HSV-2 infection. Cell Immunol. 269, 29-37 (2011).
9. Thatte, A., DeWitte-Orr, S.J., Lichty, B., Mossman, K.L., & Ashkar, A.A. A critical role for IL-15 in TLR-mediated innate antiviral immunity against genital HSV-2 infection. Immunol. Cell Biol. 89, 663-9 (2011).
10. Kwant-Mitchell, A. Ashkar, A.A., & Rosenthal, K.L. Mucosal innate and adaptive immune responses against herpes simplex virus type 2 in a humanized mouse model. J. Virol. 83, 10664-76 (2009).
11. Gallichan, W.S. & Rosenthal, K.L. Effects of the estrous cycle on local humoral immune responses and protection of intranasally immunized female nice against herpes simplex virus type 2 infection in the genital tract. Virology. 224, 487-97 (1996).