The impact of pre-analytical variables on the stability of neurofilament proteins in CSF, determined by a novel validated SinglePlex Luminex assay and ELISA

Journal of Immunological Methods

Volumn 402, Issue 1-2, 2014, Pages 43-49

  Koel Simmelink, Marleen J A ^a   Vennegoor, Anke ^a   Killestein, Joep ^a   Blankenstein, Marinus A ^a   Norgren, Niklas ^b   Korth, Carsten ^c   Teunissen, Charlotte E ^a  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UmanDiagnostics AB   (Sweden)

^c HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Cerebrospinal fluid; Neurofilament heavy (NfH) protein; Neurofilament light (NfL) protein; NfH SinglePlex Luminex assay; Pre analytical variables; Stability

Indexed keywords

2 PHENYL 3 [PARA (BETA PYRROLIDINOETHOXY)PHENYL[2,1 B]NAPHTHOFURAN; BIOLOGICAL MARKER; NEUROFILAMENT PROTEIN; NEUROFILAMENT PROTEIN H; NEUROFILAMENT PROTEIN L;

AD; ALS; ALZHEIMER DISEASE; AMYOTROPHIC LATERAL SCLEROSIS; ARTICLE; BLOOD; CEREBROSPINAL FLUID; CIS; DEMYELINATING DISEASE; ENZYME LINKED IMMUNO SORBENT ASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FREEZING; FRONTOTEMPORAL DEMENTIA; FTD; HIGH QUALITY CONTROL; HQC; HUMAN; LBD; LEWY BODIES DISEASE; LIMIT OF DETECTION; LIMIT OF LINEARITY; LOD; LOL; LOW QUALITY CONTROL; LP; LQC; LUMBAR PUNCTURE; MULTIPLE SCLEROSIS; NEUROFILAMENT; NEUROFILAMENT HEAVY; NEUROFILAMENT HEAVY (NFH) PROTEIN; NEUROFILAMENT LIGHT; NEUROFILAMENT LIGHT (NFL) PROTEIN; NFH; NFH SINGLEPLEX LUMINEX ASSAY; NFL; OBSERVER VARIATION; PRE-ANALYTICAL VARIABLES; PROTEIN STABILITY; REPRODUCIBILITY; SD; STABILITY; STANDARD DEVIATION; TEMPERATURE; TIME; VALIDATION STUDY;

AD; ALS; ALZHEIMER DISEASE; AMYOTROPHIC LATERAL SCLEROSIS; CEREBROSPINAL FLUID; CIS; CLINICALLY ISOLATED SYNDROME; CSF; ELISA; ENZYME LINKED IMMUNO SORBENT ASSAY; FRONTOTEMPORAL DEMENTIA; FTD; HIGH QUALITY CONTROL; HQC; LBD; LEWY BODIES DISEASE; LIMIT OF DETECTION; LIMIT OF LINEARITY; LOD; LOL; LOW QUALITY CONTROL; LP; LQC; LUMBAR PUNCTURE; MS; MULTIPLE SCLEROSIS; NEUROFILAMENT; NEUROFILAMENT HEAVY; NEUROFILAMENT HEAVY (NFH) PROTEIN; NEUROFILAMENT LIGHT; NEUROFILAMENT LIGHT (NFL) PROTEIN; NF; NFH; NFH SINGLEPLEX LUMINEX ASSAY; NFL; PRE-ANALYTICAL VARIABLES; SD; STABILITY; STANDARD DEVIATION;

BIOLOGICAL MARKERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FREEZING; HUMANS; NEUROFILAMENT PROTEINS; OBSERVER VARIATION; PROTEIN STABILITY; REPRODUCIBILITY OF RESULTS; TEMPERATURE; TIME FACTORS;

EID: 84889012499     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.11.008     Document Type: Article

References (26)

1. Blumenberg M. Evolution of homologous domains of cytoplasmic intermediate filament proteins and lamins. Mol. Biol. Evol. 1989, 6:53.
2. Deisenhammer F., Bartos A., Egg R., Gilhus N.E., Giovannoni G., Rauer S., et al. Guidelines on routine cerebrospinal fluid analysis report from an EFNS task force. Eur. J. Neurol. 2006, 13:913.
3. Deisenhammer F., Egg R., Giovannoni G., Hemmer B., Petzold A., Sellebjerg F., et al. EFNS guidelines on disease-specific CSF investigations. Eur. J. Neurol. 2009, 16:760-e163.
4. Gunnarsson M., Malmeström C., Axelsson M., Sundström P., Dahle C., Vrethem M., et al. Axonal damage in relapsing multiple sclerosis is markedly reduced by natalizumab. Ann. Neurol. 2011, 69:83.
5. Kirkwood T.B. Predicting the stability of biological standards and products. Biom. J. 1977, 33:736.
6. Koel-Simmelink M.J.A., Teunissen C.E., Behradkia P., Blankenstein M.A., Petzold A. The neurofilament light chain is not stable in vitro. Ann. Neurol. 2011, 69:1065.
7. Kuhle J., Regeniter A., Leppert D., Mehling M., Kappos L., Lindberg R.L.P., et al. A highly sensitive electrochemiluminescence immunoassay for the neurofilament heavy chain protein. J. Neuroimmunol. 2010, 220:114.
8. Kuhle J., Plattner K., Bestwick J.P., Lindberg R.L., Ramagopalan S.V., Norgren N., et al. A comparative study of CSF neurofilament light and heavy chain protein in MS. Mult. Scler. 2013, 1.
9. Lewis S.B., Wolper R.A., Miralia L., Yang C., Shaw G. Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients. J. Cereb. Blood Flow Metab. 2008, 28:1261.
10. Norgren N., Karlsson J.E., Rosengren L., Stigbrand T. Monoclonal antibodies selective for low molecular weight neurofilaments. Hybrid. Hybridomics 2002, 21:53.
11. Norgren N., Rosengren L., Stigbrand T. Elevated neurofilament levels in neurological diseases. Brain Res. 2003, 987:25.
12. Norgren N., Sundstrom P., Svenningsson A., Rosengren L., Stigbrand T., Gunnarsson M. Neurofilament and glial fibrillary acidic protein in multiple sclerosis. Neurology 2004, 63:1586.
13. Perret-Liaudet A., Pelpel M., Tholance Y., Dumont B., Vanderstichele H., Zorzi W., et al. Risk of Alzheimer's disease biological misdiagnosis linked to cerebrospinal collection tubes. J. Alzheimers Dis. 2012, 31:13.
14. Petzold A. Neurofilament phosphoforms: surrogate markers for axonal injury, degeneration and loss. J. Neurol. Sci. 2005, 233:183.
15. Petzold A., Keir G., Green A.J., Giovannoni G., Thompson E. A specific ELISA for measuring neurofilament heavy chain phosphoforms. J. Immunol. Methods 2003, 278:179.
16. Petzold A., Mondria T., Kuhle J., Rocca M.A., Cornelissen J., Te Boekhorst P., et al. Evidence for acute neurotoxicity after chemotherapy. Ann. Neurol. 2010, 68:806.
17. Petzold A., Altintas A., Andreoni L., Bartos A., Berthele A., Blankenstein M.A., et al. Neurofilament ELISA validation. J. Immunol. Methods 2010, 352:23.
18. Petzold A., Tisdall M.M., Girbes A.R., Martinian L., Thom M., Kitchen N., et al. In vivo monitoring of neuronal loss in traumatic brain injury: a microdialysis study. Brain 2011, 134:464.
19. Reijn T.S., Abdo W.F., Schelhaas H.J., Verbeek M.M. CSF neurofilament protein analysis in the differential diagnosis of ALS. J. Neurol. 2009, 256:615.
20. Shaw G., Yang C., Ellis R., Anderson K., Parker Mickle J., Scheff S., et al. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem. Biophys. Res. Commun. 2005, 336:1268.
21. Steinacker P., Fang L., Kuhle J., Petzold A., Tumani H., Ludolph A.C., et al. Soluble beta-amyloid precursor protein is related to disease progression in amyotrophic lateral sclerosis. PLoS One 2011, 6:e23600.
22. Terasawa K., Taguchi T., Momota R., Naito I., Murakami T., Ohtsuka A. Human erythrocytes possess a cytoplasmic endoskeleton containing β-actin and neurofilament protein. Arch. Histol. Cytol. 2006, 69:329.
23. Teunissen C., Iacobaeus E., Khademi M., Brundin L., Norgren N., Koel-Simmelink M.J. Combination of CSF N-acetylaspartate and neurofilaments in multiple sclerosis. Neurology 2009, 72:1322.
24. Wang H., Wu M., Zhan C., Ma E., Yang M., Yang X. Neurofilament proteins in axonal regeneration and neurodegenerative diseases. Neurol. Reg. Res. 2012, 7:620.
25. You J.S., Gelfanova V., Knierman M.D., Witzmann F.A., Wang M., Hale J.E. The impact of blood contamination on the proteome of cerebrospinal fluid. Proteomics 2005, 5:290.
26. Zanier E.R., Refai D., Zipfel G.J., Zoerle T., Longhi L., Esparza T.J., et al. Neurofilament light chain levels in ventricular cerebrospinal fluid after acute aneurysmal subarachnoid haemorrhage. J. Neurol. Neurosurg. Psychiatry 2011, 82:157.