Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae

Journal of Biotechnology

Volumn 168, Issue 4, 2013, Pages 486-492

  Ito, Yoichiro ^a   Yamanishi, Mamoru ^a   Ikeuchi, Akinori ^a   Imamura, Chie ^a   Tokuhiro, Kenro ^a   Kitagawa, Takao ^b   Matsuyama, Takashi ^a  

^a Rd Management Section   (Japan)

^b YAMAGUCHI UNIVERSITY   (Japan)

Author keywords

3' UTR activity; Cellulase; FI; Fluorescent protein; Metabolic engineering; OpGFP; RP; SS; Terminator region

Indexed keywords

CELLULASE; FI; FLUORESCENT PROTEIN; OPGFP; RP; SS; TERMINATOR REGION;

METABOLIC ENGINEERING; PROTEINS; STRAIN;

YEAST;

CELLULASE; GLUCAN SYNTHASE;

3' UNTRANSLATED REGION; ALCOHOL PRODUCTION; ARTICLE; BINDING SITE; CARBON SOURCE; CELL GROWTH; CELL STRESS; CONTROLLED STUDY; ENZYME SYNTHESIS; FUNGAL GENE; GENE CASSETTE; GROWTH CURVE; METABOLIC ENGINEERING; NONHUMAN; OSMOTIC STRESS; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; RHIZOPUS ORYZAE; SACCHAROMYCES CEREVISIAE; SALT STRESS; TRANSGENE;

SACCHAROMYCES CEREVISIAE;

3′-UTR ACTIVITY; CELLULASE; CODON-OPTIMIZED GREEN FLUORESCENT PROTEIN; FI; FLUORESCENCE INTENSITY; FLUORESCENT PROTEIN; METABOLIC ENGINEERING; OPGFP; RIBOSOMAL PROTEIN; RP; SIDE-SCATTER INTENSITY; SS; TERMINATOR REGION;

3' UNTRANSLATED REGIONS; BIOREACTORS; CARBON; CELLULASE; GENOME, FUNGAL; METABOLIC ENGINEERING; PROMOTER REGIONS, GENETIC; PROTEIN BIOSYNTHESIS; SACCHAROMYCES CEREVISIAE; TERMINATOR REGIONS, GENETIC; TRANSGENES;

EID: 84888844326     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.09.024     Document Type: Article

References (35)

1. Briza P., Breitenbach M., Ellinger A., Segall J. Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae. Genes Dev. 1990, 4:1775-1789.
2. Briza P., Eckerstorfer M., Breitenbach M. The sporulation-specific enzymes encoded by the DIT1 and DIT2 genes catalyze a two-step reaction leading to a soluble LL-dityrosine-containing precursor of the yeast spore wall. Proc. Natl. Acad. Sci. U.S.A. 1994, 91:4524-4528.
3. Da Silva N.A., Srikrishnan S. Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae. FEMS Yeast Res 2012, 12:197-214.
4. Decker C., Parker R. A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation. Genes Dev. 1993, 7:1632-1643.
5. Friesen H., Hepworth S., Segall J. An Ssn6-Tup1-dependent negative regulatory element controls sporulation-specific expression of DIT1 and DIT2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 1997, 17:123-134.
6. Fujita Y., Takahashi S., Ueda M., Tanaka A., Okada H., Morikawa Y., Kawaguchi T., Arai M., Fukuda H., Kondo A. Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl. Environ. Microbiol. 2002, 68:5136-5141.
7. Herrick D., Parker R., Jacobson A. Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae. Mol. Cell. Biol. 1990, 10:2269-2284.
8. Hohmann S. Characterization of PDC6, a third structural gene for pyruvate decarboxylase in Saccharomyces cerevisiae. J. Bacteriol. 1991, 173:7963-7969.
9. Ishida N., Saitoh S., Tokuhiro K., Nagamori E., Matsuyama T., Kitamoto K., Takahashi H. Efficient production of l-lactic acid by metabolically engineered Saccharomyces cerevisiae with a genome-integrated l-lactate dehydrogenase gene. Appl. Environ. Microbiol. 2005, 71:1964-1970.
10. Ito Y., Ikeuchi A., Imamura C. Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library. Protein Eng. Des. Sel. 2013, 26:73-79.
11. Jarboe L., Zhang X., Wang X., Moore J., Shanmugam K., Ingram L. Metabolic engineering for production of biorenewable fuels and chemicals: contributions of synthetic biology. J. Biomed. Biotechnol. 2010, 2010:761042.
12. Kim I.K., Roldao A., Siewers V., Nielsen J. A systems-level approach for metabolic engineering of yeast cell factories. FEMS Yeast Res. 2012, 12:228-248.
13. Kuehner J.N., Pearson E.L., Moore C. Unravelling the means to an end: RNA polymerase II transcription termination. Nat. Rev. Mol. Cell. Biol. 2011, 12:283-294.
14. Kuersten S., Goodwin E. The power of the 3' UTR: translational control and development. Nat. Rev. Genet. 2003, 4:626-637.
15. Kung Y., Runguphan W., Keasling J.D. From fields to fuels: recent advances in the microbial production of biofuels. ACS Synth. Biol. 2012, 1:498-513.
16. Lind K., Norbeck J. A QPCR-based reporter system to study post-transcriptional regulation via the 3' untranslated region of mRNA in Saccharomyces cerevisiae. Yeast 2009, 26:407-413.
17. Luengo J., Garcia B., Sandoval A., Naharro G., Olivera E. Bioplastics from microorganisms. Curr. Opin. Microbiol. 2003, 6:251-260.
18. Matsuyama T., Yamanishi M., Takahashi H. Improvement of galactose induction system in Saccharomyces cerevisiae. J. Biosci. Bioeng. 2011, 111:175-177.
19. Muhlrad D., Parker R. Mutations affecting stability and deadenylation of the yeast MFA2 transcript. Genes Dev. 1992, 6:2100-2111.
20. Morawski B., Lin Z., Cirino P., Joo H., Bandara G., Arnold F.H. Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng. 13 2000, 377-384.
21. Ohto C., Muramatsu M., Obata S., Sakuradani E., Shimizu S. Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols. Appl. Microbiol. Biotechnol. 2009, 82:837-845.
22. Partow S., Siewers V., Bjørn S., Nielsen J., Maury J. Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 2010, 27:955-964.
23. Peralta-Yahya P., Keasling J. Advanced biofuel production in microbes. Biotechnol. J. 2010, 5:147-162.
24. Richard P., Manley J.L. Transcription termination by nuclear RNA polymerases. Genes Dev. 2009, 23:1247-1269.
25. Rogowska-Wrzesinska A., Larsen P., Blomberg A., Görg A., Roepstorff P., Norbeck J., Fey S. Comparison of the proteomes of three yeast wild type strains: CEN.PK2 FY1679 and W303. Comp. Funct. Genomics 2001, 2:207-225.
26. Saitoh S., Mieno Y., Nagashima T., Kumagai C., Kitamoto K. Breeding of a new type of baker's yeast by δ-integration for overproduction of glucoamylase using a homothallic yeast. J. Ferment. Bioeng. 1996, 81:98-103.
27. Siddiqui M.S., Thodey K., Trenchard I., Smolke C.D. Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012, 12:144-170.
28. Stephanopoulos G. Metabolic engineering. Biotechnol. Bioeng. 1998, 58:119-120.
29. Sun J., Shao Z., Zhao H., Nair N., Wen F., Xu J.H. Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. Biotechnol. Bioeng. 2012, 109:2082-2092.
30. Tsai S.L., DaSilva N.A., Chen W. Functional display of complex cellulosomes on the yeast surface via adaptive assembly. ACS Synth. Biol. 2013, 2:14-21.
31. Wen F., Sun J., Zhao H.M. Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. Appl. Environ. Microbiol. 2010, 76:1251-1260.
32. Yamada R., Taniguchi N., Tanaka T., Ogino C., Fukuda H., Kondo A. Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression. Biotechnol. Biofuels 2011, 4.
33. Yamanishi M., Ito Y., Kintaka R., Imamura C., Katahira S., Ikeuchi A., Moriya H., Matsuyama T. A genome-wide activity assessment of terminator regions in Saccharomyces cerevisiae provides a "terminatome" toolbox. ACS Synth. Biol. 2013, 2:337-347.
34. Yamanishi M., Katahira S., Matsuyama T. TPS1 terminator increases mRNA and protein yield in a Saccharomyces cerevisiae expression system. Biosci. Biotechnol. Biochem. 2011, 75:2234-2236.
35. Yamanishi M., Matsuyama T. A modified Cre-lox genetic switch to dynamically control metabolic flow in transgenic Saccharomyces cerevisiae. ACS Synth. Biol. 2012, 1:172-180.