Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of recq-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III α-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis

Journal of Biological Chemistry

Volumn 288, Issue 47, 2013, Pages 33500-33508

  Pradhan, Arun ^a,b   Singh, Thiyam Ramsing ^a   Ali, Abdullah Mahmood ^a   Wahengbam, Kebola ^a   Meetei, Amom Ruhikanta ^a,c  

^a CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^c MANIPUR UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOM SYNDROME; CO-IMMUNOPRECIPITATIONS; GENOME INSTABILITY; GENOMIC INSTABILITY; MITOTIC ARREST; NUCLEAR MATRIX; PROTEIN COMPLEXES; SPINDLE-ASSEMBLY;

AMINO ACIDS; GENES; PHOSPHORYLATION; STABILITY;

PROTEINS;

BLM TOPO III ALPHA RMI1-RMI2 PROTEIN; BLOOM SYNDROME HELICASE; MONOPOLAR SPINDLE 1; PEPTIDES AND PROTEINS; RECQ HELICASE; SERINE; SERINE 112; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BLM GENE; BLOOM SYNDROME; CELL COMPONENT; CELL NUCLEOPLASM; CELL NUCLEUS MATRIX; CHROMATIN STRUCTURE; CHROMOSOMAL INSTABILITY; CONTROLLED STUDY; ENZYME ACTIVATION; GENOMIC INSTABILITY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; M PHASE CELL CYCLE CHECKPOINT; MICRONUCLEUS; MITOSIS INHIBITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN PROCESSING;

BLOOM SYNDROME; BTR COMPLEX; DNA HELICASE; GENOME INSTABILITY; POSTTRANSLATIONAL MODIFICATION; PROTEIN PHOSPHORYLATION; RMI2; SHRNA; SPINDLE ASSEMBLY CHECKPOINT (SAC); SUBCELLULAR FRACTIONATION;

AMINO ACID SUBSTITUTION; CARRIER PROTEINS; CELL CYCLE PROTEINS; CHROMOSOMAL INSTABILITY; DNA TOPOISOMERASES, TYPE I; DNA-BINDING PROTEINS; HEK293 CELLS; HELA CELLS; HUMANS; M PHASE CELL CYCLE CHECKPOINTS; MULTIPROTEIN COMPLEXES; MUTATION, MISSENSE; NUCLEAR PROTEINS; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; RECQ HELICASES; SERINE; SPINDLE APPARATUS;

EID: 84888320615     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.470823     Document Type: Article

References (16)

1. German, J., and Ellis, N. A. (2002) in The Genetic Basis of Human Cancer (Vogelstein, B., and Kinzler, K. W., eds.), 2nd Ed., pp. 267-288, McGraw-Hill, New York
2. Chu, W. K., and Hickson, I. D. (2009) RecQ helicases. Multifunctional genome caretakers. Nat. Rev. Cancer 9, 644-654
3. Wang, Y., Cortez, D., Yazdi, P., Neff, N., Elledge, S. J., and Qin, J. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14, 927-939
4. Meetei, A. R., Sechi, S., Wallisch, M., Yang, D., Young, M. K., Joenje, H., Hoatlin, M. E., and Wang, W. (2003) A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome. Mol. Cell Biol. 23, 3417-3426
5. Singh, T. R., Ali, A. M., Busygina, V., Raynard, S., Fan, Q., Du, C. H., Andreassen, P. R., Sung, P., and Meetei, A. R. (2008) BLAP18/RMI2, a novel OB-fold-containing protein, is an essential componentof the Bloom helicase-double Holliday junction dissolvasome. Genes Dev. 22, 2856-2868
6. Xu, D., Guo, R., Sobeck, A., Bachrati, C. Z., Yang, J., Enomoto, T., Brown, G. W., Hoatlin, M. E., Hickson, I. D., and Wang, W. (2008) RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability. Genes Dev. 22, 2843-2855
7. Yin, J., Sobeck, A., Xu, C., Meetei, A. R., Hoatlin, M., Li, L., and Wang, W. (2005) BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity. EMBO J. 24, 1465-1476
8. Leng, M., Chan, D. W., Luo, H., Zhu, C., Qin, J., and Wang, Y. (2006) MPS1-dependent mitotic BLM phosphorylationis important for chromosome stability. Proc. Natl. Acad. Sci. U. S. A. 103, 11485-11490
9. Musacchio, A. (2011) Spindle assembly checkpoint. The third decade. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366, 3595-3604
10. Ali, A. M., Pradhan, A., Singh, T. R., Du, C., Li, J., Wahengbam, K., Grassman, E., Auerbach, A. D., Pang, Q., and Meetei, A. R. (2012) FAAP20. A novel ubiquitin-binding FA nuclear core-complex protein required for functional integrity of the FA-BRCA DNA repair pathway. Blood 119, 3285-3294
11. Dutertre, S., Ababou, M., Onclercq, R., Delic, J., Chatton, B., Jaulin, C., and Amor-Guéret, M. (2000) Cell cycle regulationofthe endogenous wild type Bloom's syndrome DNA helicase. Oncogene 19, 2731-2738
12. Hoadley, K. A., Xu, D., Xue, Y., Satyshur, K. A., Wang, W., and Keck, J. L. (2010) Structure and cellular roles of the RMI core complex from the Bloom syndrome dissolvasome. Structure 18, 1149-1158
13. Wang, F., Yang, Y., Singh, T. R., Busygina, V., Guo, R., Wan, K., Wang, W., Sung, P., Meetei, A. R., and Lei, M. (2010) Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex. Structure 18, 1159-1170
14. Arnold, K., Bordoli, L., Kopp, J., and Schwede, T. (2006) The SWISS-MODEL workspace. A web-based environment for protein structure homology modelling. Bioinformatics 22, 195-201
15. Kiefer, F., Arnold, K., Künzli, M., Bordoli, L., and Schwede, T. (2009) The SWISS-MODEL Repository and associated resources. Nucleic Acids Res. 37, D387-D392
16. Peitsch, M. C. (1995) Protein modeling by E-mail. Nat. Biotechnol. 13, 658-660