A novel ultrasensitive bioluminescent receptor-binding assay of INSL3 through chemical conjugation with nanoluciferase

Biochimie

Volumn 95, Issue 12, 2013, Pages 2454-2459

  Zhang, Lei ^a,b   Song, Ge ^a   Xu, Ting ^a   Wu, Qing Ping ^a   Shao, Xiao Xia ^a   Liu, Ya Li ^b   Xu, Zeng Guang ^b   Guo, Zhan Yun ^a,b  

^a TONGJI UNIVERSITY   (China)

^b TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Bioluminescent assay; Chemical conjugation; INSL3; Nanoluciferase; Receptor binding

Indexed keywords

CYSTEINE; INSULIN LIKE PEPTIDE 3; LUCIFERASE; MEPYRAMINE MALEATE; NANOLUCIFERASE; PEPTIDE HORMONE; RELAXIN; RELAXIN FAMILY PEPTIDE RECEPTOR 2; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; BIOENGINEERING; BIOLUMINESCENCE; CHEMICAL MODIFICATION; CONJUGATION; DISULFIDE BOND; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING;

BIOLUMINESCENT ASSAY; CHEMICAL CONJUGATION; INSL3; NANOLUCIFERASE; RECEPTOR-BINDING;

HEK293 CELLS; HUMANS; INSULIN; LUCIFERASES; LUMINESCENT MEASUREMENTS; PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS;

EID: 84887824237     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2013.09.008     Document Type: Article

References (20)

1. R. Ivell, and R. Anand-Ivell Biological role and clinical significance of insulin-like peptide 3 Curr. Opin. Endocrinol. Diabetes Obes. 18 2011 210 216
2. K. Bay, and A.M. Andersson Human testicular insulin-like factor 3: in relation to development, reproductive hormones and andrological disorders Int. J. Androl. 34 2011 97 109
3. I.M. Adham, E. Burkhardt, M. Benahmed, and W. Engel Cloning of a cDNA for a novel insulin-like peptide of the testicular Leydig cells J. Biol. Chem. 268 1993 26668 26672
4. E. Burkhardt, I.M. Adham, U. Hobohm, D. Murphy, C. Sander, and W. Engel A human cDNA coding for the Leydig insulin-like peptide (Ley I-L) Hum. Genet. 94 1994 91 94
5. E. Burkhardt, I.M. Adham, B. Brosig, A. Gastmann, M.G. Mattei, and W. Engel Structural organization of the porcine and human genes coding for a Leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3) Genomics 20 1994 13 19
6. E.E. Büllesbach, and C. Schwabe A novel Leydig cell cDNA-derived protein is a relaxin-like factor J. Biol. Chem. 270 1995 16011 16015
7. S. Zimmermann, G. Steding, J.M. Emmen, A.O. Brinkmann, K. Nayernia, A.F. Holstein, W. Engel, and I.M. Adham Targeted disruption of the Insl3 gene causes bilateral cryptorchidism Mol. Endocrinol. 13 1999 681 691
8. S. Nef, and L.F. Parada Cryptorchidism in mice mutant for Insl3 Nat. Genet. 22 1999 295 299
9. K. Kawamura, J. Kumagai, S. Sudo, S.Y. Chun, M. Pisarska, H. Morita, J. Toppari, P. Fu, J.D. Wade, R.A. Bathgate, and A.J. Hsueh Paracrine regulation of mammalian oocyte maturation and male germ cell survival Proc. Natl. Acad. Sci. USA 101 2004 7323 7328
10. N.V. Bogatcheva, A. Truong, S. Feng, W. Engel, I.M. Adham, and A.I. Agoulnik GREAT/LGR8 is the only receptor for insulin-like 3 peptide Mol. Endocrinol. 17 2003 2639 2646
11. J. Kumagai, S.Y. Hsu, H. Matsumi, J.S. Roh, P. Fu, J.D. Wade, R.A. Bathgate, and A.J. Hsueh INSL3/Leydig insulin-like peptide activates the LGR8 receptor important in testis descent J. Biol. Chem. 277 2002 31283 31286
12. P.R. Selvin Principles and biophysical applications of lanthanide-based probes Annu. Rev. Biophys. Biomol. Struct. 31 2002 275 302
13. F. Shabanpoor, R.A. Hughes, R.A. Bathgate, S. Zhang, D.B. Scanlon, F. Lin, M.A. Hossain, F. Separovic, and J.D. Wade Solid-phase synthesis of europium-labeled human INSL3 as a novel probe for the study of ligand-receptor interactions Bioconjug. Chem. 19 2008 1456 1463
14. W.J. Zhang, X.J. Gao, Y.L. Liu, X.X. Shao, and Z.Y. Guo Design, recombinant expression and europium-labeling of a fully active easily-labeled INSL3 analog for receptor-binding assays Process. Biochem. 47 2012 1856 1860
15. M.P. Hall, J. Unch, B.F. Binkowski, M.P. Valley, B.L. Butler, M.G. Wood, P. Otto, K. Zimmerman, G. Vidugiris, T. Machleidt, M.B. Robers, H.A. Benink, C.T. Eggers, M.R. Slater, P.L. Meisenheimer, D.H. Klaubert, F. Fan, L.P. Encell, and K.V. Wood Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate ACS Chem. Biol. 7 2012 1848 1857
16. G. Song, Q. Jiang, T. Xu, Y.L. Liu, Z.G. Xu, and Z.Y. Guo A convenient luminescence assay of ferroportin internalization to study its interaction with hepcidin FEBS J. 280 2013 1773 1781
17. P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, and D.C. Klenk Measurement of protein using bicinchoninic acid Anal. Biochem. 150 1985 76 85
18. K.J. Rosengren, S. Zhang, F. Lin, N.L. Daly, D.J. Scott, R.A. Hughes, R.A. Bathgate, D.J. Craik, and J.D. Wade Solution structure and characterization of the LGR8 receptor binding surface of insulin-like peptide 3 J. Biol. Chem. 281 2006 28287 28295
19. N.C. Veitch Horseradish peroxidase: a modern view of a classic enzyme Phytochemistry 65 2004 249 259
20. C.A. Marquette, and L.J. Blum Chemiluminescent enzyme immunoassays: a review of bioanalytical applications Bioanalysis 1 2009 1259 1269