Irreproducible positive results on the Cobas AmpliPrep/Cobas TaqMan HIV-1 Qual test are different qualitatively from confirmed positive results

Journal of Medical Virology

Volumn 86, Issue 1, 2014, Pages 82-87

  Maritz, Jean ^a   van Zyl, Gert U ^a   Preiser, Wolfgang ^a  

^a UNIVERSITY OF STELLENBOSCH   (South Africa)

Author keywords

False positive; Infant diagnosis; PCR

Indexed keywords

ARTICLE; BLOOD SAMPLING; CHILD; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FLUORESCENCE ANALYSIS; FOLLOW UP; HIV TEST; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; IMMUNOLOGY TEST KIT; LABORATORY TEST; MAJOR CLINICAL STUDY; NUCLEIC ACID AMPLIFICATION; PREDICTIVE VALUE; PREDICTOR VARIABLE; PRESCHOOL CHILD; QUALITATIVE DIAGNOSIS; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; VIRUS STRAIN;

HUMAN IMMUNODEFICIENCY VIRUS 1;

FALSE POSITIVE; INFANT DIAGNOSIS; PCR;

FALSE POSITIVE REACTIONS; FEMALE; HIV INFECTIONS; HIV-1; HUMANS; INFANT; INFANT, NEWBORN; MALE; MOLECULAR DIAGNOSTIC TECHNIQUES; PREDICTIVE VALUE OF TESTS; REPRODUCIBILITY OF RESULTS; RETROSPECTIVE STUDIES; SOUTH AFRICA;

EID: 84887821111     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.23811     Document Type: Article

References (13)

1. Barron P, Pillay Y, Doherty T, Sherman G, Jackson D, Bhardwaj S, Robinson P, Goga A. 2013. Eliminating mother-to-child HIV transmission in South Africa. Bull World Health Organ 91:70-74. doi: 10.2471/BLT.12.106807
2. Feucht UD, Forsyth B, Kruger M. 2012. False-positive HIV DNA PCR testing of infants: Implications in a changing epidemic. S Afr Med J 102:149-152.
3. Lima V, Harrigan R, Montaner JS. 2009. Increased reporting of detectable plasma HIV-1 RNA levels at the critical threshold of 50 copies per milliliter with the Taqman assay in comparison to the Amplicor assay. J Acquir Immune Defic Syndr 51:3-6.
4. Maritz J, Preiser W, van Zyl GU. 2012. Establishing diagnostic cut-off criteria for the COBAS AmpliPrep/COBAS TaqMan HIV-1 Qualitative test through validation against the Amplicor DNA test v1.5 for infant diagnosis using dried blood spots. J Clin Virol 53:106-109.
5. Moodley P, Parboosing R, Moodley D. 2013. Reduction in perinatal HIV infections in KwaZulu-Natal, South Africa in the era of more effective PMTCT interventions (2004-2012). J Acquir Immune Defic Syndr 63:410-415.
6. National Department of Health, South Africa. 2010. Guidelines on the management of HIV infection in children. 2nd edition. Available from http://www.sahivsoc.org/practise-guidelines/national-dept-of-health-guidelines
7. Public Health Agency of Canada. 2010. HIV/AIDS Epi Updates, July 2010, Surveillance and Risk Assessment Division, Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada. Available from http://www.phac-aspc.gc.ca/aids-sida/publication/epi/2010/index-eng.php. Accessed July 17, 2013.
8. Public Health Ontario. CobasAmpliprep/Cobas Taqman HIV-1 Qual Test-New test for HIV1 PCR change in testing methodology/sample submission. Labstract LAB-SD-074-000, October 2011. Available from http://www.publichealthontario.ca/en/eRepository/LAB_SD_074_Cobas_amploprep_taqman_HIV1_change.pdf. Accessed July 17, 2013.
9. Roche. 2007. Roche® COBAS® AmpliPrep/COBAS® TaqMan HIV-1 Qual Test [package insert]. Branchburg, NJ. Roche.
10. Shain EB, Clemens JM. 2008. A new method for robust quantitative and qualitative analysis of real-time PCR. Nucleic Acids Res 36:e91.
11. Sherman G, Lilian R, Barron P, Candy S, Robinson P, Bhardwaj S. Laboratory information system (LIS) data is useful for monitoring the prevention of mother-to-child transmission program (PMTCT) in South Africa. In: XIX International AIDS Conference; 2012 22-27 July; Washington, United States: Abstract no. TUPE691. Available from http://www.iasociety.org/Abstracts/A200745523.aspx. Accessed July 16, 2013.
12. Violari A, Cotton MF, Gibb DM, Babiker AG, Steyn J, Madhi SA, Jean-Philippe P, McIntyre JA, CHER Study Team. 2008. Early antiretroviral therapy and mortality among HIV-infected infants. N Engl J Med 359:2233-2244.
13. Zanchetta M, Anselmi A, Vendrame D, Rampon O, Giaquinto C, Mazza A, Accapezzato D, Barnaba V, De Rossi A. 2008. Early therapy in HIV-1-infected children: Effect on HIV-1 dynamics and HIV-1-specific immune response. Antivir Ther 13:47-55.