Single step BP/LR combined Gateway reactions

BioTechniques

Volumn 55, Issue 5, 2013, Pages 265-268

  Liang, Xiquan ^a   Peng, Lansha ^a   Baek, Chang Ho ^a   Katzen, Federico ^a  

^a Life Technologies   (United States)

Author keywords

Clonase; Cloning; One pot reaction; Recombination

Indexed keywords

DNA FRAGMENT; GREEN FLUORESCENT PROTEIN;

ARTICLE; DNA SEQUENCE; GENE EXPRESSION; GENETIC RECOMBINATION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION;

BACTERIOPHAGE LAMBDA; CLONING, MOLECULAR; DNA; GENETIC VECTORS; PLASMIDS; RECOMBINATION, GENETIC;

EID: 84887577708     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114101     Document Type: Article

References (4)

1. Hartley, J.L., G.F. Temple, and M.A. Brasch. 2000. DNA cloning using in vitro site-specific recombination. Genome Res. 10:1788-1795.
2. Katzen, F. 2007. Gateway recombinational cloning: a biological operating system. Expert Opin Drug Discov. 2:571-589.
3. Landy, A. 1989. Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu. Rev. Biochem. 58:913-949.
4. Cheo, D.L., S.A. Titus, D.R. Byrd, J.L. Hartley, G.F. Temple, and M.A. Brasch. 2004. Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones. Genome Res. 14:2111-2120.