Effects of photodynamic therapy on gram-positive and gram-negative bacterial biofilms by bioluminescence imaging and scanning electron microscopic analysis

Photomedicine and Laser Surgery

Volumn 31, Issue 11, 2013, Pages 519-525

  Garcez, Aguinaldo S ^a   Núñez, Silvia C ^b   Azambuja, Nilton ^c   Fregnani, Eduardo R ^d   Rodriguez, Helena M H ^a   Hamblin, Michael R ^e   Suzuki, Hideo ^a   Ribeiro, Martha S ^b  

^a SÃO LEOPOLDO MANDIC INSTITUTE AND RESEARCH CENTER   (Brazil)

^b INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES   (Brazil)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

^d HOSPITAL SÍRIO LIBANÊS   (Brazil)

^e MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL REDUCTION; BACTERIAL VIABILITY; BIOLUMINESCENCE IMAGING; ENTEROCOCCUS FAECALIS; EXTRACELLULAR MATRICES; PHOTODYNAMIC THERAPY (PDT); PSEUDOMONAS AERUGINOSA; SCANNING ELECTRON MICROSCOPIC;

AROMATIC COMPOUNDS; BACTERIA; BIOLUMINESCENCE; CANALS; HYDRAULIC STRUCTURES; PHOSPHORESCENCE; PHOTODYNAMIC THERAPY; SCANNING ELECTRON MICROSCOPY; SEMICONDUCTOR LASERS;

BIOFILMS;

COLORING AGENT; METHYLENE BLUE; PHOTOSENSITIZING AGENT;

ARTICLE; BIOFILM; DIODE LASER; DRUG EFFECT; ENTEROCOCCUS FAECALIS; HUMAN; LUMINESCENCE; METHODOLOGY; MICROBIOLOGY; PHOTOCHEMOTHERAPY; PSEUDOMONAS AERUGINOSA; SCANNING ELECTRON MICROSCOPY; TOOTH PULP;

BIOFILMS; COLORING AGENTS; DENTAL PULP CAVITY; ENTEROCOCCUS FAECALIS; HUMANS; LASERS, SEMICONDUCTOR; LUMINESCENCE; METHYLENE BLUE; MICROSCOPY, ELECTRON, SCANNING; PHOTOCHEMOTHERAPY; PHOTOSENSITIZING AGENTS; PSEUDOMONAS AERUGINOSA;

EID: 84887428430     PISSN: 15495418     EISSN: None     Source Type: Journal    
DOI: 10.1089/pho.2012.3341     Document Type: Article

References (30)

1. Garcez, A.S., Ribeiro, M.S., Tegos, G.P., Núñez, S.C., Jorge, A.O., and Hamblin, M.R. (2007). Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection. Lasers Surg. Med. 39, 59-66.
2. Foschi, F., Fontana, C.R., Ruggiero, K., Riahi, R., Vera, A., Doukas, A.G., Pagonis, T.C., Kent, R., Stashenko, P.P., and Soukos, N.S. (2007). Photodynamic inactivation of Enterococcus faecalis in dental root canals in vitro. Lasers Surg. Med. 39, 782-787.
3. Garcez, A.S., Nuñez, S.C., Hamblin, M.R., and Ribeiro, M.S. (2008). Antimicrobial effects of photodynamic therapy on patients with necrotic pulps and periapical lesion. J. Endod. 34, 138-142.
4. Fonseca, M.B., Jú nior, P.O., Pallota, R.C., Filho, H.F., Denardin, O.V., Rapoport, A., Dedivitis, R.A., Veronezi, J.F., Genovese, W.J., and Ricardo, A.L. (2008). Photodynamic therapy for root canals infected with Enterococcus faecalis. Photomed. Laser Surg. 26, 209-213.
5. Silva Garcez. A., Nunez, S.C., Lage-Marques, J.L., Jorge, A.O., and Ribeiro, M.S. (2006). Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 102, e93-e98.
6. Bonsor, S.J., Nichol, R., Reid, T.M., and Pearson, G.J. (2006). Microbiological evaluation of photo-activated disinfection in endodontics (an in vivo study). Br. Dent. J. 25, 337-341.
7. Dahl, T.A., Midden, W.R., and Hartman, P.E. (1989). Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen, J. Bacteriol. 171, 2188-2194.
8. Friedberg, J.S. Skema, C., Baum, E.D., Burdick, J., Vinogradov, S.A., Wilson, D.F., Horan A.D., and Nachamkin, I. (2001). In vitro effects of photodynamic therapy on Aspergillus fumigatus, J. Antimicrob. Chemother. 48, 105-107.
9. Tegos, G.P., Anbe, M., Yang, C., Demidova, T.N., Satti, M., Mroz, P., Janjua, S., Gad, F., and Hamblin, M.R. (2006). Protease-stable polycationic photosensitizer conjugates between polyethyleneimine and chlorin(e6) for broad-spectrum antimicrobial photoinactivation. Antimicrob. Agents Chemother. 50, 1402-1410.
10. Phoenix, D.A., Sayed, Z., Hussain, S., Harris, F., and Wainwright, M. (2003). The phototoxicity of phenothiazinium derivates against Escherichia coli and Staphylococcus aureus. FEMS Immunol. Med. Microbiol. 39, 17-22
11. Gois, M.M., Kurachi, C., Santana, E.J.B., Mima, E.G.O., Spolidó rio, D.M.P., Pelino, J.E.P., and Salvador Bagnato, V. (2009). Susceptibility of Staphylococcus aureus to porphyrinmediated photodynamic antimicrobial chemotherapy: an in vitro study. Lasers Med. Sci. 25, 391-395.
12. Noro Filho, G.A., Casarin, R.C., Casati, M.Z., and Giovani, E.M. (2012). PDT in non-surgical treatment of periodontitis in HIV patients: a split-mouth, randomized clinical trial. Lasers Surg. Med. 44, 296-302.
13. Hayek, R.R.A., Araú jo, N.S., Gioso, M.A., Ferreira, J., Baptista-Sobrinho, C.A., Yamada, A.M., and Ribeiro, M.S. (2005). Comparative study between the effects of photodynamic therapy and conventional therapy on microbial reduction in ligature-induced peri-implantitis in dogs. J. Periodontol. 76, 1275-1281.
14. Baptista, A., Kato, I.T., Prates, R.A., Suzuki, L.C., Raele M.P., Freitas, A.Z., and Ribeiro, M.S. (2012). Antimicrobial photodynamic therapy as a strategy to arrest enamel demineralization: a short-term study on incipient caries in a rat model. Photochem. Photobiol. 88, 584-589.
15. Wilson, M. (2004). Lethal photosensitization o oral bactéria and its potential application in the photodynamic therapy of oral infections. J. Photochem. Photobiol. B: biol. 3, 412- 418.
16. Zanin, I.C., Lobo, M.M., Rodrigues, L.K., Pimenta, L.A., Höfling, J.F., and Gonçalves, R.B. (2006). Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. Eur. J, Oral Sci. 114, 64-69.
17. Farbod, F., Kanaan, H., and Farbod, J. (2009). Infective endocarditis and antibiotic prophylaxis prior to dental/oral procedures: latest revision to the guidelines by the American Heart Association published April 2007. Int. J. Oral Maxillofac. Surg. 38, 626-631.
18. Sanchez-Rodriguez, F., Rivera, R., Suarez-Gonzalez, J., Gonzalez-Claudio, G. (2008). Prevention of infective endocarditis: a review of the American Heart Association guidelines. Bol. Assoc. Med. P. R. 100, 25-28.
19. Zanin, I.C., Gonçalves, R.B., Junior, A.B., Hope, C.K., Pratten, J. (2005). Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J. Antimicrob. Chemother. 56, 324-330.
20. Maoz, A., Mayr, R., Bresolin, G., Neuhaus, K., Francis, K.P., and Scherer, S. (2002). Sensitive in situ monitoring of a recombinant bioluminescent Yersinia enterocolitica reporter mutant in real time on Camembert cheese. Appl. Environ. Microbiol. 68, 5737-5740.
21. Garcez, A.S., Nuñez, S.C., Lage-Marques, J.L., Hamblin, M.R., and Ribeiro, M.S. (2007). Photonic real-time monitoring of bacterial reduction in root canals by genetically engineered bacteria after chemomechanical endodontic therapy. Braz. Dent. J. 18, 202-207.
22. Garcez, A.S., Núñez, S.C., Baptista, M.S., Daghastanli, N.A., Itri, R., Hamblin, M.R., and Ribeiro, M.S. (2011). Antimicrobial mechanisms behind photodynamic effect in the presence of hydrogen peroxide. Photochem. Photobiol. Sci. 10, 483-490.
23. Stuart, C.H., Schwartz, S.A., Beeson, T.J., and Owatz, C.B. (2006). Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J. Endod. 32, 93-98.
24. Ranta, K., Haapasalo, M., and Ranta, H. (1988). Monoinfection of root canal with Pseudomonas aeruginosa. Endod. Dent. Traumatol. 4, 269-272.
25. Haapasalo, M., Ranta, H., and Ranta, K.T. (1983). Facultative gram-negative enteric rods in persistent periapical infections. Acta Odontol. Scand. 41, 19-22.
26. Di Poto, A., Sbarra, M.S., Provenza, G., Visai, L., and Speziale, P. (2009). The effect of photodynamic treatment combined with antibiotic action or host defence mechanisms on Staphylococcus aureus biofilms. Biomaterials. 30, 3158-3166.
27. Sharma, M., Visai, L., Bragheri, F., Cristiani, I., Gupta, P.K., and Speziale, P. (2008). Toluidine blue-mediated photodynamic effects on staphylococcal biofilms. Antimicrob. Agents Chemother. 52, 299-305.
28. Parsons, C., McCoy, C.P., Gorman, S.P., Jones, D.S., Bell, S.E., Brady, C., McGlinchey, S.M. (2009). Anti-infective photodynamic biomaterials for the prevention of intraocular lens-associated infectious endophthalmitis. Biomaterials. 30, 597-602
29. Stepanović, S., Vuković, D., Hola, V., Di Bonaventura, G., Djukić, S., Cirković, I., and Ruzicka, F. (2004). Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Lett. Appl. Microbiol. 38, 428-432.
30. Shen, Y., Qian, W., Chung, C., Olsen, I., and Haapasalo, M. (2009). Evaluation of the effect of two chlorhexidine preparations on biofilm bacteria in vitro: a three-dimensional quantitative analysis. J. Endod. 35, 981-985.