Identification of the gene for β-fructofuranosidase from Ceratocystis moniliformis CMW 10134 and characterization of the enzyme expressed in saccharomyces cerevisiae

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  van Wyk, Niël ^a   Trollope, Kim M ^a   Steenkamp, Emma T ^b   Wingfield, Brenda D ^b   Volschenk, Heinrich ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

^b UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

fructofuranosidase; Ceratocystis moniliformis; Heterologous expression; Saccharomyces cerevisiae; Short chain fructooligosaccharides

Indexed keywords

CARBOHYDRATE SOURCES; CERATOCYSTIS MONILIFORMIS; FRUCTO-OLIGOSACCHARIDES; GLYCOSIDE HYDROLASES; HETEROLOGOUS EXPRESSION; PHYLOGENETIC ANALYSIS; TEMPERATURE CONDITIONS; VOLUMETRIC PRODUCTION;

AMINO ACIDS; CHAINS; ENZYMES; PROTEINS; SUGAR (SUCROSE); YEAST;

GENE EXPRESSION;

BETA FRUCTOFURANOSIDASE; FRUCTOSE OLIGOSACCHARIDE; FUNGAL PROTEIN; LITHIUM CHLORIDE; RECOMBINANT PROTEIN; SUCROSE;

AMINO ACID SEQUENCE; ARTICLE; CERATOCYSTIS MONILIFORMIS; CLADISTICS; CONTROLLED STUDY; DELETION MUTANT; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SYNTHESIS; FUNGAL GENE; FUNGAL GENOME; FUNGUS; FUNGUS GROWTH; GENE DELETION; GENE EXPRESSION; GENE IDENTIFICATION; GENE SEQUENCE; HYDROLYSIS; MOLECULAR CLONING; MOLECULAR PHYLOGENY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN EXPRESSION; PROTEIN MOTIF; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

CERATOCYSTIS MONILIFORMIS; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; ASCOMYCOTA; BETA-FRUCTOFURANOSIDASE; BIOTRANSFORMATION; CLONING, MOLECULAR; DNA, FUNGAL; GENE DELETION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PHYLOGENY; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SUCROSE;

EID: 84887370443     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-100     Document Type: Article

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